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The strengths and weaknesses of live fluorescently labelled algae (LFLA) to estimate herbivory in protozooplankton and mixoplankton

2022Journal article Restricted access
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dc.contributor.authorFerreira, Guilherme Duarte
dc.contributor.authorFigueira, Joana
dc.contributor.authorMarques, Sónia Cotrim
dc.contributor.authorHansen, Per Juel
dc.contributor.authorCalbet, Albert
dc.date.accessioned2022-08-11T13:29:48Z
dc.date.available2022-08-11T13:29:48Z
dc.date.issued2022
dc.descriptionAcknowledgments : This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 766327. This document reflects only the author’s view; the REA and the European Commission are not responsible for any use that may be made of the information it contains. This project is a contribution of the Marine Zooplankton Ecology Group from the Generalitat de Catalunya (2017 SGR 87) with the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S). Thanks for financial support are also due to MARE (UIDB/04292/2020 + UIDP/04292/2020), to FCT/MEC through national funds, and the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020. The authors declare no competing interests.pt_PT
dc.description.abstractThe Live Fluorescently Labelled Algae (LFLA) technique has been used numerous times to estimate microzooplankton herbivory. Yet, it is unknown how mixoplankton (i.e., single-cell organisms that can combine phototrophy and phagotrophy) affect the outcome of this technique. Hence, we conducted a broad-spectrum assessment of the strengths and weaknesses of the LFLA technique, using several mixoplanktonic and protozooplanktonic grazers. Species from different taxonomic groups and different feeding mechanisms were tested in short-term experiments (ca. 5 h) in the laboratory, at different prey concentrations and during light and dark periods of the day. Overall, our findings suggest that the LFLA technique, due to its short-term nature, is an effective tracker of diel ingestion and digestion rates, and can detect new mixoplanktonic predators. We recommend that, irrespective of the prey concentration, incubations to measure grazing rates with this technique should generally be concluded within 1 h (adaptable to the environmental temperature). Nevertheless, our results also call for caution whenever using LFLA in the field: feeding mechanisms other than direct engulfment (like peduncle feeding) may provide severely biased ingestion rates. Furthermore, size and species selectivity are very hard to circumvent. To reduce the effects of selectivity, we propose the combined use of two distinctly coloured fluorochromes (i.e., distinct emission spectra). With this modification, one could either label different size ranges of prey or account for species-specific interactions in the food web.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGuilherme Duarte Ferreira, Joana Figueira, Sónia Cotrim Marques, Per Juel Hansen, Albert Calbet, The strengths and weaknesses of Live Fluorescently Labelled Algae (LFLA) to estimate herbivory in protozooplankton and mixoplankton, Marine Environmental Research, Volume 174, 2022, 105558, ISSN 0141-1136, https://doi.org/10.1016/j.marenvres.2022.105558pt_PT
dc.identifier.doi10.1016/j.marenvres.2022.105558pt_PT
dc.identifier.issn0141-1136
dc.identifier.urihttp://hdl.handle.net/10400.8/7534
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationMarine and Environmental Sciences Centre
dc.relationMarine and Environmental Sciences Centre
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0141113622000034pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectLive FLApt_PT
dc.subjectProtistpt_PT
dc.subjectDiel feeding rhythmspt_PT
dc.subjectGrazingpt_PT
dc.subjectDigestionpt_PT
dc.subjectMixotrophypt_PT
dc.subjectMixoplanktonpt_PT
dc.titleThe strengths and weaknesses of live fluorescently labelled algae (LFLA) to estimate herbivory in protozooplankton and mixoplanktonpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04292%2F2020/PT
oaire.citation.endPage12pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMarine Environmental Researchpt_PT
oaire.citation.volume174pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameDuarte Ferreira
person.familyNameFigueira
person.familyNameCotrim Marques
person.familyNameHansen
person.familyNameCalbet
person.givenNameGuilherme
person.givenNameJoana
person.givenNameSónia
person.givenNamePer Juel
person.givenNameAlbert
person.identifierC-2471-2018
person.identifier312329
person.identifier138047
person.identifier.ciencia-idD017-9023-9519
person.identifier.ciencia-id9612-8F45-A674
person.identifier.ciencia-idE115-8500-DDFA
person.identifier.orcid0000-0003-0010-4558
person.identifier.orcid0000-0001-5896-3860
person.identifier.orcid0000-0002-9467-3825
person.identifier.orcid0000-0003-0228-9621
person.identifier.orcid0000-0003-1069-212X
person.identifier.ridC-2471-2018
person.identifier.ridE-9969-2011
person.identifier.ridA-7779-2008
person.identifier.scopus-author-id57211559216
person.identifier.scopus-author-id9271998100
person.identifier.scopus-author-id57982795900
person.identifier.scopus-author-id7003400774
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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