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The therapeutic potential of naturally occurring peptides in counteracting SH-SY5Y cells injury

2022Journal article Open access
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dc.contributor.authorPerlikowska, Renata
dc.contributor.authorSilva, Joana
dc.contributor.authorAlves, Celso
dc.contributor.authorSusano, Patrícia
dc.contributor.authorPedrosa, Rui
dc.date.accessioned2023-09-04T11:54:56Z
dc.date.available2023-09-04T11:54:56Z
dc.date.issued2022
dc.descriptionThis research was funded by the National Science Center (NCN, Miniatura, No DEC-2021/05/X/ST5/00294), PolishNationalAgency forAcademic Exchange (BPN/BPT/2021/1/00053), the Portuguese Foundation for Science and Technology (FCT) through the projects granted to MARE—Marine and Environmental Sciences Centre (UIDP/04292/2020 and UIDB/04292/2020), Associate Laboratory ARNET (LA/P/0069/2020) and CROSS-ATLANTIC (PTDC/BIA-OUT/29250/2017) project, co-financed by European Regional Development Fund (FEDER), through the Operational-Programme for Competitiveness and Internationalization (COMPETE 2020). This study was also supported by Programme for Cooperation in Science between Portugal and Poland through project NEURONS—Natural products as key drivers in the development of nEUropROtective therapeutic ageNtS (FCT/DRI/PNAAE 2021.09608.CBM).pt_PT
dc.description.abstractPeptides have revealed a large range of biological activities with high selectivity and efficiency for the development of new drugs, including neuroprotective agents. Therefore, this work investigates the neuroprotective properties of naturally occurring peptides, endomorphin-1 (EM-1), endomorphin-2 (EM-2), rubiscolin-5 (R-5), and rubiscolin-6 (R-6). We aimed at answering the question of whether wellknown opioid peptides can counteract cell injury in a common in vitromodel of Parkinson’s disease (PD). Antioxidant activity of these four peptides was evaluated by the 2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activity, oxygen radical absorbance capacity (ORAC), and ferric-reducing antioxidant power (FRAP) assays, while neuroprotective effects were assessed in a neurotoxic model induced by 6-hydroxydopamine (6-OHDA) in a human neuroblastoma cell line (SH-SY5Y). The mechanisms associated with neuroprotection were investigated by the determination of mitochondrial membrane potential (MMP), reactive oxygen species (ROS) production, and Caspase-3 activity. Among the tested peptides, endomorphins significantly prevented neuronal death induced by 6-OHDA treatment, decreasing MMP (EM-1) or Caspase-3 activity (EM-2). Meanwhile, R-6 showed antioxidant potential by FRAP assay and exhibited the highest capacity to recover the neurotoxicity induced by 6-OHDA via attenuation of ROS levels and mitochondrial dysfunction. Generally, we hypothesize that peptides’ ability to suppress the toxic effect induced by 6-OHDA may be mediated by different cellular mechanisms. The protective effect caused by endomorphins results in an antiapoptotic effect (mitochondrial protection and decrease in Caspase-3 activity), while R-6 potency to increase a cell’s viability seems to be mediated by reducing oxidative stress. Our results may provide new insight into neurodegeneration and support the short peptides as a potent drug candidate to treat PD. However, further studies should be conducted on the detailed mechanisms of how tested peptides could suppress neuronal injuries.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPerlikowska, R.; Silva, J.; Alves, C.; Susano, P.; Pedrosa, R. The Therapeutic Potential of Naturally Occurring Peptides in Counteracting SH-SY5Y Cells Injury. Int. J. Mol. Sci. 2022, 23, 11778. https://doi.org/ 10.3390/ijms231911778pt_PT
dc.identifier.doi10.3390/ijms231911778pt_PT
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.8/8753
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMarine and Environmental Sciences Centre
dc.relationMarine and Environmental Sciences Centre
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/19/11778pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectOpioid peptidespt_PT
dc.subject6-hydroxydopaminept_PT
dc.subjectNeuronal survivalpt_PT
dc.subjectNeuroprotectionpt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectApoptosispt_PT
dc.titleThe therapeutic potential of naturally occurring peptides in counteracting SH-SY5Y cells injurypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT
oaire.citation.issue19pt_PT
oaire.citation.titleInternational Journal o f Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePerlikowska
person.familyNameSilva
person.familyNameAlves
person.familyNameFrederico Susano
person.familyNamePedrosa
person.givenNameRenata
person.givenNameJoana
person.givenNameCelso
person.givenNamePatrícia
person.givenNameRui
person.identifier98885
person.identifier159091
person.identifier349272
person.identifier.ciencia-id5B14-594A-86D2
person.identifier.ciencia-id6317-6F15-BA35
person.identifier.ciencia-id501C-F268-2E60
person.identifier.ciencia-id581E-146A-9945
person.identifier.ciencia-id3817-33DE-919E
person.identifier.orcid0000-0001-5430-3953
person.identifier.orcid0000-0003-1224-1699
person.identifier.orcid0000-0003-1581-2127
person.identifier.orcid0000-0003-1426-2071
person.identifier.orcid0000-0003-0970-0575
person.identifier.ridB-3366-2017
person.identifier.ridB-4815-2015
person.identifier.scopus-author-id56370819800
person.identifier.scopus-author-id55654969100
person.identifier.scopus-author-id7005010300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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