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Identification of Asparagopsis armata‐associated bacteria and characterization of their bioactive potential

2019Journal article Open access
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dc.contributor.authorAlves, Celso
dc.contributor.authorPinteus, Susete
dc.contributor.authorLopes, Cláudia
dc.contributor.authorFino, Nádia
dc.contributor.authorSilva, Joana
dc.contributor.authorRibeiro, Joana
dc.contributor.authorRodrigues, Daniel
dc.contributor.authorFrancisco, João
dc.contributor.authorRodrigues, Américo
dc.contributor.authorPedrosa, Rui
dc.contributor.authorHorta, André
dc.date.accessioned2020-07-17T13:59:20Z
dc.date.available2020-07-17T13:59:20Z
dc.date.issued2019
dc.descriptionFP7 Food, Agriculture and Fisheries, Biotechnology, Grant/Award Number: 265896; Fundação para a Ciência e a Tecnologia, Grant/Award Number: PTDC/MAR‐BIO/6149/2014, SAICTPAC/0019/2015 ‐ LISBOA‐01‐0145‐ FEDER‐016405, SFRH/BD/103255/2014, SFRH/BD/120250/2016, SFRH/ BD/96203/2013, SFRH/BD/97764/2013 and UID/MAR/04292/2019; Centro 2020 program, Portugal 2020, European Union, European Regional Development Fund, Grant/Award Number: Centro‐01‐0145‐ FEDER‐000018pt_PT
dc.description.abstractMacroalgae‐associated bacteria have already proved to be an interesting source of compounds with therapeutic potential. Accordingly, the main aim of this study was to characterize Asparagopsis armata‐associated bacteria community and evaluate their capacity to produce substances with antitumor and antimicrobial potential. Bacteria were selected according to their phenotype and isolated by the streak plate technique. The identification was carried out by the RNA ribosomal 16s gene amplification through PCR techniques. The antimicrobial activities were evaluated against seven microorganisms (Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Salmonella enteritidis, Staphylococcus aureus, Saccharomyces cerevisiae, Candida albicans) by following their growth through spectrophotometric readings. Antitumor activities were evaluated in vitro on human cell lines derived from hepatocellular (HepG‐2) and breast carcinoma (MCF‐7) using the MTT method. The present work identified a total of 21 bacteria belonging to the genus Vibrio, Staphylococcus, Shewanella, Alteromonadaceae, Bacillus, Cobetia, and Photobacterium, with Vibrio being the most abundant (42.86%). The extract of Shewanella sp. ASP 26 bacterial strain induced the highest antimicrobial activity, namely against Bacillus subtilis and Staphylococcus aureus with an IC50 of 151.1 and 346.8 μg/mL, respectively. These bacteria (Shewanella sp.) were also the ones with highest antitumor potential, demonstrating antiproliferative activity on HepG‐2 cells. Asparagopsis armata‐associated bacteria revealed to be a potential source of compounds with antitumor and antibacterial activity.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHorta, A., Alves, C., Pinteus, S., Lopes, C., Fino, N., Silva, J., Ribeiro, J., Rodrigues, D., Francisco, J., Rodrigues, A., & Pedrosa, R. (2019). Identification of Asparagopsis armata-associated bacteria and characterization of their bioactive potential. MicrobiologyOpen, 8(11), e00824. https://doi.org/10.1002/mbo3.824pt_PT
dc.identifier.doidoi.org/10.1002/mbo3.824pt_PT
dc.identifier.issn2045-8827
dc.identifier.urihttp://hdl.handle.net/10400.8/5023
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationPTDC/MAR‐BIO/6149/2014pt_PT
dc.relationPRECISION ONCOLOGY BY INNOVATIVE THERAPIES AND TECHNOLOGIES
dc.relationNeuroprotective effects of molecules isolated from Bifurcaria bifurcata and Codium tomentosum in an in vitro and in vivo Parkinson Disease model.
dc.relationEvaluation of the benefit/risk associated to the consumption of seaweed based on the bioaccessibility/bioavailability of omega 3 fatty acids, essential elements, antioxidant compounds and contaminants
dc.relationADD VALUE TO THE MARINE INVADERS ASPARAGOPSIS ARMATA AND SARGASSUM MUTICUM
dc.relationMARE - Marine and Environmental Sciences Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAlgaept_PT
dc.subjectAntimicrobialpt_PT
dc.subjectAntitumorpt_PT
dc.subjectEpiphytic bacteria,pt_PT
dc.subjectMarine compoundspt_PT
dc.subjectMarine symbiosispt_PT
dc.titleIdentification of Asparagopsis armata‐associated bacteria and characterization of their bioactive potentialpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePRECISION ONCOLOGY BY INNOVATIVE THERAPIES AND TECHNOLOGIES
oaire.awardTitleNeuroprotective effects of molecules isolated from Bifurcaria bifurcata and Codium tomentosum in an in vitro and in vivo Parkinson Disease model.
oaire.awardTitleEvaluation of the benefit/risk associated to the consumption of seaweed based on the bioaccessibility/bioavailability of omega 3 fatty acids, essential elements, antioxidant compounds and contaminants
oaire.awardTitleADD VALUE TO THE MARINE INVADERS ASPARAGOPSIS ARMATA AND SARGASSUM MUTICUM
oaire.awardTitleMARE - Marine and Environmental Sciences Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/SAICTPAC%2F0019%2F2015/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMAR%2F04292%2F2019/PT
oaire.citation.titleMicrobiology Openpt_PT
oaire.citation.volume8pt_PT
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