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Effects of umbilical cord tissue mesenchymal stem cells (UCX®) on rat sciatic nerve regeneration after neurotmesis injuries

2014-04-30Artigo de investigação Acesso aberto
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datacite.subject.fosCiências Médicas::Biotecnologia Médica
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorGärtner, A.
dc.contributor.authorPereira, T.
dc.contributor.authorArmada-da-Silva, P. A. S.
dc.contributor.authorAmado, S.
dc.contributor.authorVeloso, A. P.
dc.contributor.authorAmorim, I.
dc.contributor.authorRibeiro, J.
dc.contributor.authorSantos, J. D.
dc.contributor.authorBárcia, R. N.
dc.contributor.authorCruz, P.
dc.contributor.authorCruz, H.
dc.contributor.authorLuís, A. L.
dc.contributor.authorSantos, J. M.
dc.contributor.authorGeuna, S.
dc.contributor.authorMaurício, A. C.
dc.date.accessioned2026-07-16T16:12:24Z
dc.date.available2026-07-16T16:12:24Z
dc.date.issued2014-04-30
dc.descriptionJournal of Stem Cells & Regenerative Medicine, 30 Apr 2014, 10(1):14-26 https://doi.org/10.46582/jsrm.1001004 PMID: 25075157 PMCID: PMC4112274
dc.descriptionThe authors would like to acknowledge the financial support from Project TRIBONE, Nº 11458, co-financed by the European Community FEDER fund through ON2 - O Novo Norte – North Portugal Regional Operational Program 2007-2013 and from the program COMPETE – Programa Operacional Factores de Competitividade, project Pest-OE/AGR/UI0211/2011, from projects QREN I&DT Cluster in Development of Products for Regenerative Medicine and Cell Therapies– Projects Biomat & Cell QREN 2008/1372 and ECBio QREN 2008/1467, complemented with ECBio S.A. own research funds. Andrea Gartner has a Doctoral grant from Fundação para a Ciência e Tecnologia (FCT) do Ministério da Educação e da Ciência SFRH/BD/70211/2010. The authors would like to acknowledge the financial support from project PTDC/SAUTOX/110457/2009 from FCT and from project "Hybrid Nanostructured Hydrogels: Bone regeneration using Multifunctional injectable Hydrogels - Rebone" – ENMED/0002/2010 from Fundação para a Ciência e Tecnologia (FCT), Ministério da Educação e da Ciência and Program Project Euronanomed, Ref: EraNet - EuroNanoMed JTC2010.
dc.description
dc.description.abstractAbstract Peripheral nerves have the intrinsic capacity of self-regeneration after traumatic injury but the extent of the regeneration is often very poor. Increasing evidence demonstrates that mesenchymal stem/stromal cells (MSCs) may play an important role in tissue regeneration through the secretion of soluble trophic factors that enhance and assist in repair by paracrine activation of surrounding cells. In the present study, the therapeutic value of a population of umbilical cord tissue-derived MSCs, obtained by a proprietary method (UCX®), was evaluated on end-to-end rat sciatic nerve repair. Furthermore, in order to promote both, end-to-end nerve fiber contacts and MSC cell-cell interaction, as well as reduce the flush away effect of the cells after administration, a commercially available haemostatic sealant, Floseal®, was used as vehicle. Both, functional and morphologic recoveries were evaluated along the healing period using extensor postural thrust (EPT), withdrawal reflex latency (WRL), ankle kinematics analysis, and either histological analysis or stereology, in the hyper-acute, acute and chronic phases of healing. The histological analysis of the hyper-acute and acute phase studies revealed that in the group treated with UCX® alone the Wallerian degeneration was improved for the subsequent process of regeneration, the fiber organization was higher, and the extent of fibrosis was lower. The chronic phase experimental groups revealed that treatment with UCX® induced an increased number of regenerated fibers and thickening of the myelin sheet. Kinematics analysis showed that the ankle joint angle determined for untreated animals was significantly different from any of the treated groups at the instant of initial contact (IC). At opposite toe off (OT) and heel rise (HR), differences were found between untreated animals and the groups treated with either UCX® alone or UCX® administered with Floseal®. Overall, the UCX® application presented positive effects in functional and morphologic recovery, in both the acute and chronic phases of the regeneration process. Kinematics analysis has revealed positive synergistic effects brought by Floseal® as vehicle for MSCs.eng
dc.description.sponsorship- Project TRIBONE, Nº 11458 - Cell Therapies- Projects Biomat & Cell QREN 2008/1372 - ECBio QREN 2008/1467 - Program Project Euronanomed, Ref: EraNet - EuroNanoMed JTC2010 (sem correspondência)
dc.identifier.citationGärtner, A., Pereira, T., Armada-da-Silva, P. A. S., Amado, S., Veloso, A. P., Amorim, I., Ribeiro, J., Santos, J. D., Bárcia, R. N., Cruz, P., Cruz, H., Luís, A. L., Santos, J. M., Geuna, S., & Maurício, A. C. (2014). Effects of umbilical cord tissue mesenchymal stem cells (UCX®) on rat sciatic nerve regeneration after neurotmesis injuries. Journal of Stem Cells & Regenerative Medicine, 10(1), 14–26. https://doi.org/10.46582/jsrm.1001004
dc.identifier.doi10.46582/jsrm.1001004
dc.identifier.issn0973-7154
dc.identifier.urihttp://hdl.handle.net/10400.8/16613
dc.language.isoeng
dc.peerreviewedyes
dc.publisherM/S GN Corporation Co. Ltd
dc.relationStrategic Project - UI 211 - 2011-2012
dc.relationPERIPHERIC NERVE SYSTEM REGENERATION THROUGH TISSUE ENGINEERING: ASSOCIATION BETWEEN BIOMATERIALS AND STEM CELLS
dc.relationDevelopment of 3D culture models for human ucmMSC-derived hepatocytes for predictive toxicology and cell therapy
dc.relationHybrid Nanostuctured Hydrogels: Bone regeneration using multifunctional injectable hydrogels
dc.relation.hasversionhttps://europepmc.org/article/med/25075157
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectFunctional assessment
dc.subjectMesenchymal stem cells
dc.subjectMyelinization
dc.subjectNeurotmesis
dc.subjectStereology
dc.subjectWharton's jelly
dc.titleEffects of umbilical cord tissue mesenchymal stem cells (UCX®) on rat sciatic nerve regeneration after neurotmesis injurieseng
dc.typeresearch article
dspace.entity.typePublication
oaire.awardNumberPEst-OE/AGR/UI0211/2011
oaire.awardNumberSFRH/BD/70211/2010
oaire.awardNumberPTDC/SAU-TOX/110457/2009
oaire.awardNumberENMED/0002/2010
oaire.awardTitleStrategic Project - UI 211 - 2011-2012
oaire.awardTitlePERIPHERIC NERVE SYSTEM REGENERATION THROUGH TISSUE ENGINEERING: ASSOCIATION BETWEEN BIOMATERIALS AND STEM CELLS
oaire.awardTitleDevelopment of 3D culture models for human ucmMSC-derived hepatocytes for predictive toxicology and cell therapy
oaire.awardTitleHybrid Nanostuctured Hydrogels: Bone regeneration using multifunctional injectable hydrogels
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FAGR%2FUI0211%2F2011/PT
oaire.awardURIhttp://hdl.handle.net/10400.8/16611
oaire.awardURIhttp://hdl.handle.net/10400.8/16612
oaire.awardURIhttp://hdl.handle.net/10400.8/16236
oaire.citation.endPage26
oaire.citation.issue1
oaire.citation.startPage14
oaire.citation.titleJournal of Stem Cells and Regenerative Medicine
oaire.citation.volume10
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso para Projectos de I&D em todos os Domínios Científicos - 2009
oaire.fundingStreamEuroNanoMed - Second Joint Call 2010
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameAmado
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person.identifier.ridO-6988-2016
person.identifier.scopus-author-id16038616600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
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