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Protein differential expression induced by endocrine disrupting compounds in a terrestrial isopod

2010-04Journal article Restricted access
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datacite.subject.fosCiências Médicas::Outras Ciências Médicas
datacite.subject.fosCiências Naturais::Ciências da Terra e do Ambiente
datacite.subject.fosCiências Naturais::Ciências Químicas
datacite.subject.sdg02:Erradicar a Fome
datacite.subject.sdg08:Trabalho Digno e Crescimento Económico
datacite.subject.sdg15:Proteger a Vida Terrestre
dc.contributor.authorLemos, Marco F. L.
dc.contributor.authorEsteves, Ana Cristina
dc.contributor.authorSamyn, Bart
dc.contributor.authorTimperman, Isaak
dc.contributor.authorvan Beeumen, Jozef
dc.contributor.authorCorreia, António
dc.contributor.authorvan Gestel, Cornelis A. M.
dc.contributor.authorSoares, Amadeu M. V. M.
dc.date.accessioned2025-12-18T12:32:53Z
dc.date.available2025-12-18T12:32:53Z
dc.date.issued2010-04
dc.description.abstractEndocrine disrupting compounds (EDCs) have been studied due to their impact on human health and increasing awareness of their impact on wildlife species. Studies concerning the organ-specific molecular effects of EDC in invertebrates are important to understand the mechanisms of action of this class of toxicants but are scarce in the literature. We have used a dose/response approach to unravel the protein expression in different organs of isopods exposed to bisphenol A (BPA) and vinclozolin (Vz) and assess their potential use as surrogate species. Male isopods were exposed to a range of Vz or of BPA concentrations. After animal dissection, proteins were extracted from gut, hepatopancreas and testes. Protein profiles were analysed by electrophoresis and differentially expressed proteins were identified by MALDI mass spectrometry. EDCs affected proteins involved in the energy metabolism (arginine kinase), proteins of the heat shock protein family (Hsp70 and GRP78) and most likely microtubule dynamics (tubulin). Different proteins expressed at different concentrations in different organs are indicative of the organ-specific effects of BPA and Vz. Additionally, several proteins were up-regulated at lower but not higher BPA or Vz concentrations, bringing new data to the non-monotonic response curve controversy. Furthermore, our findings suggest that some common responses to EDCs in both vertebrates and invertebrates may exist.eng
dc.description.sponsorshipThis work was supported by fellowships provided to MFL Lemos (BD/13868/2003) and AC Esteves (BPD-38008/2007) by the Fundação Portuguesa para a Ciência e Tecnologia. B. Samyn is a postdoctoral fellow of the Fund for Scientific Research-Flanders (F.W.O.-Vlaanderen, Belgium). Parts of this work were supported by FCT Project Grant reference PTDC/BIA-BDE/75690/2006.
dc.identifier.citationMarco F.L. Lemos, Ana Cristina Esteves, Bart Samyn, Isaak Timperman, Jozef van Beeumen, António Correia, Cornelis A.M. van Gestel, Amadeu M.V.M. Soares, Protein differential expression induced by endocrine disrupting compounds in a terrestrial isopod, Chemosphere, Volume 79, Issue 5, 2010, Pages 570-576, ISSN 0045-6535, https://doi.org/10.1016/j.chemosphere.2010.01.055.
dc.identifier.doi10.1016/j.chemosphere.2010.01.055
dc.identifier.eissn1879-1298
dc.identifier.issn0045-6535
dc.identifier.urihttp://hdl.handle.net/10400.8/15150
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationTHE USE OF BIOCHEMICAL AND MOLECULAR BIOMARKERS TO ASSESS THE EFFECTS OF ENDOCRINE DISRUPTOR COMPOUNDS IN SOIL ORGANISMS. LINKING LABORATORY TO FIELD EXPOSURE
dc.relationDisrupTox - Edaphic Endocrine Disruption Assessment Tool Box
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S0045653510001190?via%3Dihub
dc.relation.ispartofChemosphere
dc.rights.uriN/A
dc.subjectEcotoxicoproteomics
dc.subjectSoil invertebrates
dc.subjectBisphenol A
dc.subjectVinclozolin
dc.subjectEcotoxicology
dc.titleProtein differential expression induced by endocrine disrupting compounds in a terrestrial isopodeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTHE USE OF BIOCHEMICAL AND MOLECULAR BIOMARKERS TO ASSESS THE EFFECTS OF ENDOCRINE DISRUPTOR COMPOUNDS IN SOIL ORGANISMS. LINKING LABORATORY TO FIELD EXPOSURE
oaire.awardTitleDisrupTox - Edaphic Endocrine Disruption Assessment Tool Box
oaire.awardURIhttp://hdl.handle.net/10400.8/15148
oaire.awardURIhttp://hdl.handle.net/10400.8/15073
oaire.citation.endPage576
oaire.citation.issue5
oaire.citation.startPage570
oaire.citation.titleChemosphere
oaire.citation.volume79
oaire.fundingStreamConcurso para Projectos de I&D em todos os Domínios Científicos - 2006
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLemos
person.givenNameMarco
person.identifier996337
person.identifier.ciencia-id971F-ACCA-C0D1
person.identifier.orcid0000-0001-9887-1864
person.identifier.ridF-7951-2011
person.identifier.scopus-author-id7006042884
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relation.isAuthorOfPublication.latestForDiscoveryf21e5540-df76-43e9-ad64-93edd70da1f1
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Endocrine disrupting compounds (EDCs) have been studied due to their impact on human health and increasing awareness of their impact on wildlife species. Studies concerning the organ-specific molecular effects of EDC in invertebrates are important to understand the mechanisms of action of this class of toxicants but are scarce in the literature. We have used a dose/response approach to unravel the protein expression in different organs of isopods exposed to bisphenol A (BPA) and vinclozolin (Vz) and assess their potential use as surrogate species. Male isopods were exposed to a range of Vz or of BPA concentrations. After animal dissection, proteins were extracted from gut, hepatopancreas and testes. Protein profiles were analysed by electrophoresis and differentially expressed proteins were identified by MALDI mass spectrometry. EDCs affected proteins involved in the energy metabolism (arginine kinase), proteins of the heat shock protein family (Hsp70 and GRP78) and most likely microtubule dynamics (tubulin). Different proteins expressed at different concentrations in different organs are indicative of the organ-specific effects of BPA and Vz. Additionally, several proteins were up-regulated at lower but not higher BPA or Vz concentrations, bringing new data to the non-monotonic response curve controversy. Furthermore, our findings suggest that some common responses to EDCs in both vertebrates and invertebrates may exist.
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