Repository logo
 
Publication

Uncovering the bioactivity of Aurantiochytrium sp.: a comparison of extraction methodologies

2021Journal article Restricted access
Simple item page
dc.contributor.authorReboleira, João
dc.contributor.authorFélix, Rafael
dc.contributor.authorVicente, Tânia F. L.
dc.contributor.authorJanuário, Adriana P.
dc.contributor.authorFélix, Carina
dc.contributor.authorMelo, Marcelo M.R. de
dc.contributor.authorSilva, Carlos M.
dc.contributor.authorRibeiro, Ana C.
dc.contributor.authorSaraiva, Jorge A.
dc.contributor.authorBandarra, Narcisa M.
dc.contributor.authorSapatinha, Maria
dc.contributor.authorPaulo, Maria C.
dc.contributor.authorCoutinho, Joana
dc.contributor.authorLemos, Marco F.L.
dc.date.accessioned2022-08-22T12:51:40Z
dc.date.available2022-08-22T12:51:40Z
dc.date.issued2021
dc.descriptionFunding This study had the support of Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2020 to MARE, UIDB/50011/2020 & UIDP/50011/2020 to CICECO, and the University of Aveiro and FCT/MCT for the fnancial support for LAQV-REQUIMTE research Unit (FCT UIDB/50006/2020) through national founds, and, where applicable, co-fnanced by the FEDER, within the PT2020 Partnership Agreement. This work was also funded by the project “Valorização dos subprodutos do processo biotecnológico de produção de esqualeno e DHA pela microalga Aurantiochytrium sp.” (AlgaValue) (POCI-01-0247-FEDER-017680) supported by COMPETE 2020, and Integrated Programme of SR&TD “SmartBioR” (reference Centro-01–0145-FEDER-000018) cofunded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund. The authors also thank the funding from Project AgroForWealth (CENTRO-01–0145-FEDER-000001), funded by Centro2020, through FEDER and PT2020 and University of Aveiro.pt_PT
dc.description.abstractAurantiochytrium sp. is an emerging alternative source of polyunsaturated fatty acids (PUFAs), docosahexaenoic acid (DHA), and squalene, playing an important role in the phasing out of traditional fish sources for these compounds. Novel lipid extraction techniques with a focus on sustainability and low environmental footprint are being developed for this organism, but the exploration of other added-value compounds within it is still very limited. In this work, a combination of novel green extraction techniques (high hydrostatic pressure extraction (HPE) and supercritical fluid extraction (SFE)) and traditional techniques (organic solvent Soxhlet extraction and hydrodistillation (HD)) was used to obtain lipophilic extracts of Aurantiochytrium sp., which were then screened for antioxidant (DPPH radical reduction capacity and ferric-reducing antioxidant potential (FRAP) assays), lipid oxidation protection, antimicrobial, anti-aging enzyme inhibition (collagenase, elastase and hyaluronidase), and anti-inflammatory (inhibition of NO production) activities. The screening revealed promising extracts in nearly all categories of biological activity tested, with only the enzymatic inhibition being low in all extracts. Powerful lipid oxidation protection and anti-inflammatory activity were observed in most SFE samples. Ethanolic HPEs inhibited both lipid oxidation reactions and microbial growth. The HD extract demonstrated high antioxidant, antimicrobial, and antiinflammatory activities making, it a major contender for further studies aiming at the valorization of Aurantiochytrium sp. Taken together, this study presents compelling evidence of the bioactive potential of Aurantiochytrium sp. and encourages further exploration of its composition and application.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationReboleira, J., Félix, R., Vicente, T.F.L. et al. Uncovering the Bioactivity of Aurantiochytrium sp.: a Comparison of Extraction Methodologies. Mar Biotechnol 24, 40–54 (2022). https://doi.org/10.1007/s10126-021-10085-wpt_PT
dc.identifier.doi10.1007/s10126-021-10085-wpt_PT
dc.identifier.issn1436-2228
dc.identifier.issn1436-2236
dc.identifier.urihttp://hdl.handle.net/10400.8/7571
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationUID/MAR/04292/2020pt_PT
dc.relationCICECO-Aveiro Institute of Materials
dc.relationCICECO-Aveiro Institute of Materials
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s10126-021-10085-w#citeaspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHigh hydrostatic pressurept_PT
dc.subjectSupercritical CO2pt_PT
dc.subjectHydrodistillationpt_PT
dc.subjectAntioxidantpt_PT
dc.subjectAntimicrobialpt_PT
dc.subjectAnti-agingpt_PT
dc.subjectAnti-inflammatorypt_PT
dc.titleUncovering the bioactivity of Aurantiochytrium sp.: a comparison of extraction methodologiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCICECO-Aveiro Institute of Materials
oaire.awardTitleCICECO-Aveiro Institute of Materials
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50011%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50011%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.citation.endPage54pt_PT
oaire.citation.startPage40pt_PT
oaire.citation.titleMarine Biotechnologypt_PT
oaire.citation.volume24pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMendes Reboleira
person.familyNameFaria da Costa Félix
person.familyNameVicente
person.familyNameJanuário
person.familyNameFaria Da Costa Félix
person.familyNameLemos
person.givenNameJoão
person.givenNameRafael Alexandre
person.givenNameTânia F. L.
person.givenNameAdriana P.
person.givenNameCarina Rafaela
person.givenNameMarco
person.identifierKWTr8tQAAAAJ
person.identifier996337
person.identifier.ciencia-idA014-8343-2B4E
person.identifier.ciencia-idA315-5CB0-097C
person.identifier.ciencia-idC61D-61EF-4328
person.identifier.ciencia-id241B-FEA3-3BED
person.identifier.ciencia-id2015-2A43-2055
person.identifier.orcid0000-0003-3261-6857
person.identifier.orcid0000-0003-4766-3736
person.identifier.orcid0000-0003-1805-3023
person.identifier.orcid0000-0002-3228-922X
person.identifier.orcid0000-0002-9813-9400
person.identifier.orcid0000-0001-9887-1864
person.identifier.ridF-7951-2011
person.identifier.scopus-author-id57200377641
person.identifier.scopus-author-id57211164252
person.identifier.scopus-author-id7006042884
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbe4114d5-4394-45ce-87bb-7724cfefb23f
relation.isAuthorOfPublicationd4d70589-8897-4538-b004-bfe2ce623b69
relation.isAuthorOfPublication9bf49c5d-35a8-44ff-b38f-1f85120d9fc6
relation.isAuthorOfPublication576c1b04-ad90-4771-80f0-e42f080e3b2b
relation.isAuthorOfPublicationbe3b35be-8af5-4b3b-aaf3-12d6cf143985
relation.isAuthorOfPublicationf21e5540-df76-43e9-ad64-93edd70da1f1
relation.isAuthorOfPublication.latestForDiscoverybe3b35be-8af5-4b3b-aaf3-12d6cf143985
relation.isProjectOfPublicationd3efc322-9e6c-4893-b063-dce718ca7023
relation.isProjectOfPublicatione912f48e-b4b0-4a0f-b060-de6cd99f40cc
relation.isProjectOfPublication49eb7a0c-4c73-4544-8b3d-cb4cf75e403c
relation.isProjectOfPublication.latestForDiscovery49eb7a0c-4c73-4544-8b3d-cb4cf75e403c

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Reboleira et al_2021_Marine Biotechnology.pdf
Size:
1.37 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.32 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

MARE - Artigos com peer review
ESTM - Artigos em revistas internacionais