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Neuroprotective potential of Myrciaria plinioides D. Legrand extract in an in vitro human neuroblastoma model

2019Journal article Restricted access
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dc.contributor.authorMarmitt, Diorge Jonatas
dc.contributor.authorAlves, Celso
dc.contributor.authorSilva, Joana
dc.contributor.authorPinteus, Susete
dc.contributor.authorSchneider, Taiane
dc.contributor.authorSantos, Roberto Christ Vianna
dc.contributor.authorFreitas, Elisete Maria de
dc.contributor.authorPedrosa, Rui
dc.contributor.authorLaufer, Stefan
dc.contributor.authorGoettert, Márcia Inês
dc.date.accessioned2020-07-17T14:00:17Z
dc.date.available2020-07-17T14:00:17Z
dc.date.issued2019
dc.description.abstractNeurodegenerative diseases are multifactorial debilitating disorders of the nervous system affecting approximately 30 million individuals worldwide. Mitochondrial dysfunction and oxidative stress have also been implicated in causing neurodegeneration. As life expectancy is increasing, neurodegenerative disorders are becoming a major social issue. None of the drugs currently available for treatment are capable of healing the patient. This means that new molecules should be explored. Plants have been used for treatment of countless medical conditions and extensive research is being carried out on species of the Myrtaceae family, widely used in traditional medicine. To date, Myrciaria plinioides D. Legrand has not been studied for its therapeutic use. To evaluate the neuroprotective effect of aqueous and ethanol extracts of this plant, we investigated the protective effects in human neuroblastoma cells (SH-SY5Y). High-performance liquid chromatography fingerprinting of extracts revealed the presence of phenolic compounds and flavonoids. Extracts showed antioxidant activity in the ORAC, DPPH, FRAP and GAE methods. Ethanol extract presented a strong inhibitory activity toward p38 and JNK3 MAPKs and AChE activity and also toward TNF-α release in human whole blood. None of the extracts significantly affected cell viability; the ethanol extract, however, reversed 6-OHDA-induced toxicity. Particularly the ethanol extract suggests neuroprotective effects by preventing membrane depolarization and by significantly decreasing H2O2 production and caspase-3 activity. The present results indicate that the ethanol extract protects SH-SY5Y cells against oxidative damage and apoptosis, as shown by the antioxidative activity of the extract as well as by the inhibition of important proteins such as caspase-3, p38 and JNK3 and the cytokine TNF-α.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMarmitt, D.J., Alves, C., Silva, J., Pinteus, S., Schneider, T., Santos, R.C.V., Freitas, E.M., Pedrosa, R., Laufer, S., & Goettert, M.I. (2019). Neuroprotective potential of Myrciaria plinioides D. Legrand extract in an in vitro human neuroblastoma model. Inflammopharmacology, 28. 10.1007/s10787-019-00652-y.pt_PT
dc.identifier.doidoi.org/10.1007/s10787-019-00652-ypt_PT
dc.identifier.issn0925-4692
dc.identifier.urihttp://hdl.handle.net/10400.8/5025
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Verlagpt_PT
dc.subjectMedicinal plantspt_PT
dc.subjectNeurodegenerative diseasespt_PT
dc.subjectHuman neuroblastoma cellspt_PT
dc.subject6-Hydroxydopaminept_PT
dc.subjectMAPKpt_PT
dc.titleNeuroprotective potential of Myrciaria plinioides D. Legrand extract in an in vitro human neuroblastoma modelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleInflammopharmacologypt_PT
oaire.citation.volume28pt_PT
person.familyNameAlves
person.familyNameSilva
person.familyNamePinteus
person.familyNamePedrosa
person.givenNameCelso
person.givenNameJoana
person.givenNameSusete
person.givenNameRui
person.identifier159091
person.identifier98885
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person.identifier.ciencia-id3817-33DE-919E
person.identifier.orcid0000-0003-1581-2127
person.identifier.orcid0000-0003-1224-1699
person.identifier.orcid0000-0002-3290-3111
person.identifier.orcid0000-0003-0970-0575
person.identifier.ridB-3366-2017
person.identifier.ridB-4815-2015
person.identifier.scopus-author-id55654969100
person.identifier.scopus-author-id56370819800
person.identifier.scopus-author-id55655328300
person.identifier.scopus-author-id7005010300
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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