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Endocrine and physiological effects of linuron and S-metolachlor in zebrafish developing embryos

2017-05Journal article Restricted access
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datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg13:Ação Climática
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authorQuintaneiro, C.
dc.contributor.authorPatrício, D.
dc.contributor.authorNovais, Sara
dc.contributor.authorSoares, A.M.V.M.
dc.contributor.authorMonteiro, M.S.
dc.date.accessioned2025-09-04T15:33:21Z
dc.date.available2025-09-04T15:33:21Z
dc.date.issued2017-05
dc.description.abstractEvaluation of the effects of linuron and S-metolachlor on apical, biochemical and transcriptional endpoints in zebrafish (Danio rerio) early life stages was the main purpose of this work. Embryos were exposed for 96h to a range of concentrations of each herbicide to determine lethal and sub-lethal effects on apical (e.g. malformations, hatching) and biochemical parameters (cholinesterase, ChE; catalase, CAT; glutathione S-transferase, GST; lipid peroxidation, LPO and lactate dehydrogenase, LDH). To evaluate endocrine disruption effects, embryos were exposed during 96h to 0.88mg/L linuron and 9.66mg/L S-metolachlor, isolated or in binary mixture. Expression of a suite of genes involved in HPT, HPG and HPA-axis was then assessed. Highest concentration of linuron (5.0mg/L) decreased hatching rate to 5% and 70.0mg/L S-metolachlor completely inhibited hatching, about 100%. Both herbicides impaired development by inducing several malformations (100% in 5.0mg/L linuron and 70.0mg/L S-metolachlor). Linuron only affected GST and CAT at concentrations of 0.25 and 0.0025mg/L, respectively. S-metolachlor induced GST (to 256%), inhibited ChE (to 61%) and LDH (to 60%) and reduced LPO levels (to 63%). Linuron isolated treatment seems to have an estrogenic mode of action due to the observed induction of vtg1. Exposure to S-metolachlor seems to interfere with steroidogenesis and with HPT and HPA-axis, since it has inhibited cyp19a2, TSHβ and CRH gene expression. In addition to vtg1 induction and CRH inhibition, herbicide combination also induced sox9b that has a role in regulation of sexual development in zebrafish. This study pointed out adverse effects of linuron and S-metolachlor, namely impairment of neurotransmission and energy production, induction of steroidogenesis, and interference with HPT and HPA-axis. These results contributed to elucidate modes of action of linuron and S-metolachlor in zebrafish embryo model. Furthermore, gene expression patterns obtained are indicative of endocrine disruption action of these herbicides.eng
dc.description.sponsorshipWe acknowledge the financial support of Fundação para a Ciência e Tecnologia (FCT) through the post- doctoral fellowships of C. Quintaneiro (SFRH/BPD/89951/2012), M.S. Monteiro (SFRH/BPD/100448/2014) and S.C. Novais (SFRH/BPD/94500/2013). We acknowledge the financial support to CESAM (UID/AMB/50017) and to MARE (UID/MAR/04292/2013), to FCT/MEC through national funds, and the co-funding by the FEDER (POCI-01-0145-FEDER-00763), within the PT2020 Partnership Agreement and Compete 2020.
dc.identifier.citationQuintaneiro C, Patrício D, Novais SC, Soares AMVM, Monteiro MS. Endocrine and physiological effects of linuron and S-metolachlor in zebrafish developing embryos. Sci Total Environ. 2017 May 15;586:390-400. doi: 10.1016/j.scitotenv.2016.11.153. Epub 2017 Feb 13. PMID: 28209406
dc.identifier.doi10.1016/j.scitotenv.2016.11.153
dc.identifier.issn0048-9697
dc.identifier.urihttp://hdl.handle.net/10400.8/13986
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier BV
dc.relationENVIRONOME - INTEGRATING OMICS IN ECOTOXICOLOGY: TOOLS FOR ENVIRONMENTAL RISK ASSESSMENT
dc.relationCentre for Environmental and Marine Studies
dc.relationSFRH/BPD/94500/2013
dc.relationUID/AMB/50017
dc.relationUID/MAR/04292/2013
dc.relationPOCI-01-0145-FEDER-00763
dc.relation.hasversionhttps://pubmed.ncbi.nlm.nih.gov/28209406/
dc.relation.ispartofScience of The Total Environment
dc.rights.uriN/A
dc.subjectAlternative methods
dc.subjectEndocrine disruption
dc.subjectFish embryo toxicity
dc.subjectNeurotoxicity
dc.subjectOxidative stress
dc.subjectTeratogenic effects
dc.titleEndocrine and physiological effects of linuron and S-metolachlor in zebrafish developing embryoseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleENVIRONOME - INTEGRATING OMICS IN ECOTOXICOLOGY: TOOLS FOR ENVIRONMENTAL RISK ASSESSMENT
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F94500%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAMB%2F50017%2F2013/PT
oaire.citation.endPage400
oaire.citation.startPage390
oaire.citation.titleScience of the Total Environment
oaire.citation.volume586
oaire.fundingStreamFARH
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameNovais
person.givenNameSara
person.identifier.ciencia-id2216-A2E0-A118
person.identifier.orcid0000-0003-1306-3396
person.identifier.ridA-9032-2012
person.identifier.scopus-author-id23025463300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublicationde69a91d-24ba-4155-ad23-76367cca2232
relation.isAuthorOfPublication.latestForDiscoveryde69a91d-24ba-4155-ad23-76367cca2232
relation.isProjectOfPublication4f84bfd4-842e-4f1e-8fe6-55bc16089f83
relation.isProjectOfPublication6e070485-f065-40ca-88be-6bce4e06fdd9
relation.isProjectOfPublication.latestForDiscovery4f84bfd4-842e-4f1e-8fe6-55bc16089f83

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