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Cold atmospheric plasma activation of human gingival fibroblasts for improved wound healing

2024-10-18Journal article Restricted access
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datacite.subject.fosCiências Médicas::Ciências da Saúde
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorSilva, Neusa
dc.contributor.authorMarques, Joana
dc.contributor.authorBrito da Cruz, Mariana
dc.contributor.authorLuis, Henrique
dc.contributor.authorMata, António
dc.contributor.authorSério, Susana
dc.date.accessioned2025-09-04T15:22:10Z
dc.date.available2025-09-04T15:22:10Z
dc.date.issued2024-10-18
dc.descriptionSpecial Issue on Application of Cold Atmospheric Plasma in Human and Veterinary Disease.
dc.descriptionArticle number - ad83de
dc.description.abstractSoft tissue regeneration plays a crucial role after oral surgery, as the successful healing of the soft tissue is a primary indicator of an efficacious intervention. Cold atmospheric plasma (CAP) has recently emerged as a promising therapeutic modality, exhibiting notable effects on cell migration and proliferation. Despite its potential, the dental application of CAP remains underexplored. This in vitro study aims to elucidate the impact of CAP activated medium on human gingival fibroblast responses, for future wound healing strategies. The study was divided into four parts: initial characterization of the plasma Jet, assessment of cell concentration, exploration of treatment distance effects, and treatment time dynamics. Human gingival fibroblasts were exposed to complete DMEM medium (without sodium pyruvate) activated with CAP at treatment distances of 2, 5, 7, and 9 mm, and treatment times of 15, 60, 120, 180, and 300 s for 1, 2 and 3 d of culture. The cell viability was evaluated using resazurin-based method, while wound healing dynamics was assessed via the scratch assay technique using phase-contrast microscopy. The cell morphology was characterised through fluorescence microscopy using propidium iodide and phalloidin staining, complemented by scanning electron microscopy. The results revealed that treatment distances and exposure times can influence the cell behaviour depending on the cell concentration. For the selected concentration of 1 × 104 cells ml−1, a treatment distance of 9 mm appeared to enhance human gingival fibroblast viability compared to a treatment distance of 2 mm and the control group. The images revealed adherent cells with a pattern typical of fibroblasts. However, no differences were observed for exposure times of 15 s and 180 s. The observed results further evidence that the exposure of the medium to the CAP device promoted an increase in cell viability, proliferation, and attachment in human gingival fibroblasts.eng
dc.description.sponsorshipAcknowledgment Neusa Silva sincerely acknowledge Fundação para a Ciência e a Tecnologia (FCT) for the PhD Scholarship—Cold Atmospheric Plasma for periodontal and peri-implant disease treatment: antibacterial effects and cellular response, 2022. 13655.BD. The authors also acknowledge the financial support from FEDER, through Programa Operacional Factores de Competitividade − COMPETE and FCT, for the project UIDB/04559/2020 (DOI 10.54499/UIDB/04559/2020).
dc.identifier.citationSilva, Neusa & Marques, Joana & da Cruz, Mariana & Luis, Henrique & Mata, António & Sério, S.. (2024). Cold atmospheric plasma activation of human gingival fibroblasts for improved wound healing. Journal of Physics D: Applied Physics. 58. 10.1088/1361-6463/ad83de.
dc.identifier.doi10.1088/1361-6463/ad83de
dc.identifier.eissn1361-6463
dc.identifier.issn0022-3727
dc.identifier.urihttp://hdl.handle.net/10400.8/13985
dc.language.isoeng
dc.peerreviewedyes
dc.publisherIOP Publishing
dc.relationLaboratory for Instrumentation, Biomedical Engineering and Radiation Physics
dc.relation.hasversionhttps://iopscience.iop.org/article/10.1088/1361-6463/ad83de#back-to-top-target
dc.relation.ispartofJournal of Physics D: Applied Physics
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBiomedical applications
dc.subjectCold atmospheric plasma
dc.subjectGingival regeneration
dc.subjectDentistry
dc.subjectHuman gingival fibroblasts
dc.subjectPlasma medicine
dc.titleCold atmospheric plasma activation of human gingival fibroblasts for improved wound healingeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleLaboratory for Instrumentation, Biomedical Engineering and Radiation Physics
oaire.awardURIhttp://hdl.handle.net/10400.8/13984
oaire.citation.issue2
oaire.citation.titleJournal of Physics D: Applied Physics
oaire.citation.volume58
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Base
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLuis
person.givenNameHenrique
person.identifier.ciencia-id9618-0E44-341F
person.identifier.orcid0000-0002-1092-7825
person.identifier.ridI-7551-2018
person.identifier.scopus-author-id6602751823
relation.isAuthorOfPublicationad05c605-e71c-49fa-8a79-61bd1f3518f8
relation.isAuthorOfPublication.latestForDiscoveryad05c605-e71c-49fa-8a79-61bd1f3518f8
relation.isProjectOfPublication5615741f-ecdd-48ec-b08c-35dd8185c843
relation.isProjectOfPublication.latestForDiscovery5615741f-ecdd-48ec-b08c-35dd8185c843

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