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Unveiling the Cultivation of Nostoc sp. under Controlled Laboratory Conditions

2024-04Journal article Open access
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dc.contributor.authorMouga, Teresa
dc.contributor.authorPereira, Jéssica
dc.contributor.authorMoreira, Vitória
dc.contributor.authorAfonso, Clélia
dc.date.accessioned2024-04-29T14:54:49Z
dc.date.available2024-04-29T14:54:49Z
dc.date.issued2024-04
dc.date.updated2024-04-28T16:01:25Z
dc.descriptionThis work was funded by national funds through FCT—Fundação para a Ciência e a Tecnologia for I.P., within the scope of MARE (https://doi.org/10.54499/UIDP/04292/2020; https://doi.org/10.54499/UIDB/04292/2020) and https://doi.org/10.54499/LA/P/0069/2020, granted to the Associate Laboratory ARNET.pt_PT
dc.description.abstractCyanobacteria, photoautotrophic Gram-negative bacteria, play a crucial role in aquatic and terrestrial environments, contributing significantly to fundamental ecological processes and displaying potential for various biotechnological applications. It is, therefore, critical to identify viable strains for aquaculture and establish accurate culture parameters to ensure an extensive biomass supply for biotechnology purposes. This study aims to establish optimal laboratory batch culture conditions for Nostoc 136, sourced from Alga2O, Coimbra, Portugal. Preliminary investigations were conducted to identify the optimal culture parameters and to perform biomass analysis, including protein and pigment content. The highest growth was achieved with an initial inoculum concentration of 1 g.L−1, using modified BG11 supplemented with nitrogen, resulting in a Specific Growth Rate (SGR) of 0.232 ± 0.017 µ.day−1. When exposed to white, red, and blue LED light, the most favourable growth occurred under a combination of white and red LED light exhibiting an SGR of 0.142 ± 0.020 µ.day−1. The protein content was determined to be 10.80 ± 2.09%. Regarding the pigments, phycocyanin reached a concentration of 200.29 ± 30.07 µg.mL−1, phycoerythrin 148.29 ± 26.74 µg.mL−1, and allophycocyanin 10.69 ± 6.07 µg.mL−1. This study underscores the influence of light and nutrient supplementation on the growth of the Nostoc biomass.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citation[MDPI] Mouga, T.; Pereira, J.; Moreira, V.; Afonso, C. Desvendando o Cultivo de Nostoc sp. sob condições controladas de laboratório. Biologia 2024, 13, 306. https://doi.org/10.3390/biology13050306pt_PT
dc.identifier.doihttps://doi.org/10.3390/biology13050306pt_PT
dc.identifier.eissn2079-7737
dc.identifier.slugcv-prod-4073573
dc.identifier.urihttp://hdl.handle.net/10400.8/9631
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMarine and Environmental Sciences Centre
dc.relationMarine and Environmental Sciences Centre
dc.relationAquatic Research Infrastructure Network
dc.relation.publisherversionhttps://www.mdpi.com/2079-7737/13/5/306pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectNostoc sp. 136pt_PT
dc.subjectInitial densitypt_PT
dc.subjectNutrient supplementationpt_PT
dc.subjectIrradiancept_PT
dc.subjectSpecific growth ratept_PT
dc.subjectProductivitypt_PT
dc.titleUnveiling the Cultivation of Nostoc sp. under Controlled Laboratory Conditionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleAquatic Research Infrastructure Network
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0069%2F2020/PT
oaire.citation.issue5pt_PT
oaire.citation.startPage306pt_PT
oaire.citation.titleBiologypt_PT
oaire.citation.volume13pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMouga
person.familyNameAfonso
person.givenNameTeresa
person.givenNameClélia
person.identifier153274
person.identifier.ciencia-idE312-FDCC-1ED8
person.identifier.ciencia-idC512-13C3-50DD
person.identifier.orcid0000-0003-4849-8840
person.identifier.orcid0000-0002-4167-5237
person.identifier.ridB-1211-2016
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaidE312-FDCC-1ED8 | Teresa Mouga
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication901e8c7b-9818-4493-8e96-75d0c5ad718c
relation.isAuthorOfPublication4876cc93-8744-4885-ae53-7b552036195d
relation.isAuthorOfPublication.latestForDiscovery901e8c7b-9818-4493-8e96-75d0c5ad718c
relation.isProjectOfPublicationa66a15eb-ff92-44d8-a34e-0f87f0c01d01
relation.isProjectOfPublication54154db1-7a14-4ce1-b54e-b3a01fcd44f5
relation.isProjectOfPublication2b6d255a-da1a-4dc7-b08f-361bcf19995e
relation.isProjectOfPublication.latestForDiscovery2b6d255a-da1a-4dc7-b08f-361bcf19995e

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