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Abieta-7,13-Diene in Nematode-Infected Pinewood Pinus pinaster Branch Extracts: Isolation and the Elucidation and Characterization of Its Structure

2025-01-01Journal article Open access
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datacite.subject.fosEngenharia e Tecnologia::Engenharia Química
datacite.subject.fosCiências Naturais::Ciências Químicas
datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg15:Proteger a Vida Terrestre
dc.contributor.authorGaspar, Marisa C.
dc.contributor.authorCruz, Pedro F.
dc.contributor.authorBrito, Rui M. M.
dc.contributor.authorSousa, Hermínio C. de
dc.contributor.authorBraga, Mara E. M.
dc.date.accessioned2025-09-08T15:25:58Z
dc.date.available2025-09-08T15:25:58Z
dc.date.issued2025-01-01
dc.descriptionThis article belongs to the Special Issue Advance in Pine Wilt Disease Acknowledgments The authors are grateful to Fátima Isabel Antunes Nunes from the Laboratory of Mass Spectrometry (LEM) integrated in the National Mass Spectrometry Network (RNEM) of Portugal for performing the LC-MS analyses and to forest engineer José Bernardino Dias from National Forest Services (Instituto de Conservação da Natureza e Florestas, ICNF) for the identification of the PWD-symptomatic trees and the collection of branch samples for this study. The authors are also grateful to designer José Gomes for his work in the preparation of Figure 1.
dc.descriptionArticle number - 61
dc.description.abstractThe oleoresin and volatile fraction produced by conifers, such as Pinus pinaster, play a crucial role in plant defence, acting as precursors to resin acids and adapting in response to environmental stress or pathogen attacks. Abietadiene (abieta-7,13-diene), the biosynthetic precursor to abietic acid, has been identified as the most abundant compound in extracts from pinewood nematode (PWN)-infected P. pinaster trees. As abietadiene is not commercially available, this study aimed to achieve, for the first time, its isolation, structure elucidation, and detailed characterization from readily available forestry residues. Abietadiene was successfully isolated using thin-layer chromatography (TLC), and its purity and identity were evaluated using multiple analytical techniques: gas chromatography (GC), liquid chromatography (LC), nuclear magnetic resonance (NMR), and Fourier-transform infrared spectroscopy (FTIR). GC analysis indicated a purity of over 70% for the isolated compound, while LC provided the higher purity value of 98%. The identity of abietadiene was unequivocally confirmed through LC, FTIR, and NMR analysis. This work represents the first isolation and comprehensive characterization of abietadiene from a natural source, making detailed chemical data on this compound available to the scientific community. These findings may be used for future studies on the biological interactions and ecological roles of abietadiene, particularly in the context of plant defence and pathogen resistance.eng
dc.description.sponsorshipThis research was funded by COMPETE 2020, Fundação para a Ciência e Tecnologia (FCT, Portugal), through the Project ECOVECTOR—POCI-01-0145-FEDER-016820, CERES (PEst-C/EQB/UI0102/2019, UIDB/00102/2020, and UIDP/00102/2020), and ciTechCare (UIDB/05704/2020 and UIDP/05704/2020). M. C. Gaspar thanks FCT for financial support (CEECINST/00060/2021). NMR data were collected at the UC-NMR facility, which is supported in part by FEDER—European Regional Development Fund through COMPETE; FCT (RECI/QEQ-QFI/0168/2012, CENTRO-07-CT62-FEDER-002012); Rede Nacional de Ressonância Magnética Nuclear (RNRMN); and Coimbra Chemistry Centre (UID/QUI/00313/2019).
dc.identifier.citationGaspar, M.C.; Cruz, P.F.; Brito, R.M.M.; De Sousa, H.C.; Braga, M.E.M. Abieta-7,13-Diene in Nematode-Infected Pinewood Pinus pinaster Branch Extracts: Isolation and the Elucidation and Characterization of Its Structure. Forests 2025, 16, 61. https://doi.org/10.3390/f16010061
dc.identifier.doi10.3390/f16010061
dc.identifier.issn1999-4907
dc.identifier.urihttp://hdl.handle.net/10400.8/14011
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationChemical Process Engineering and Forest Products Research Centre
dc.relationCenter for Innovative Care and Health Technology
dc.relationUC-NMR: a voyage from chemical space to biological function
dc.relationCoimbra Chemistry Center
dc.relation.hasversionhttps://www.mdpi.com/1999-4907/16/1/61
dc.relation.ispartofForests
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectabietadiene
dc.subjectPinus pinaster
dc.subjectisolation
dc.subjectbranch extract
dc.subjectstructure elucidation
dc.subjectplant defence
dc.subjectpine wilt disease
dc.subjectabieta-7
dc.subject13-diene
dc.titleAbieta-7,13-Diene in Nematode-Infected Pinewood Pinus pinaster Branch Extracts: Isolation and the Elucidation and Characterization of Its Structureeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleChemical Process Engineering and Forest Products Research Centre
oaire.awardTitleCenter for Innovative Care and Health Technology
oaire.awardTitleUC-NMR: a voyage from chemical space to biological function
oaire.awardTitleCoimbra Chemistry Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00102%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05704%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/RECI%2FQEQ-QFI%2F0168%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F00313%2F2019/PT
oaire.citation.issue1
oaire.citation.titleForests
oaire.citation.volume16
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream5876-PPCDTI
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameda Costa Gaspar
person.givenNameMarisa
person.identifier.ciencia-idA71E-6EFA-80FE
person.identifier.orcid0000-0002-5584-6324
person.identifier.scopus-author-id55969188500
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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