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Antifungal and Antioxidant Properties of Chitosan Polymers Obtained from Nontraditional Polybius henslowii Sources
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Advisor(s)
Abstract(s)
Chitin was extracted from Polybius henslowii, a swimming crab, captured in large
quantities throughout the Portuguese coast by purse seine vessels as bycatch. After standard chitin
extraction procedures, water-soluble chitosan products were obtained via two di erent methods:
(1) N-acetylation with the addition of acetic anhydride and (2) a reaction with hydrogen peroxide.
The chemical structure and molecular weight of chitosan derivatives, water-soluble chitosan (WSC)
and chitooligosaccharides (COS), were confirmed by Fourier Transform Infrared Spectroscopy (FT-IR)
and gel permeation chromatography (GPC).Antioxidant and metal chelation activities were evaluated,
and the growth inhibition capacity was tested on four phytopatogens. The chitooligosaccharides
from pereopods (pCOS) and shell body parts (sCOS) inhibited all fungal species tested, particularly
Cryphonectria parasitica with 84.7% and 85.5%, respectively. Both radical scavenging and antifungal
activities proved to be dose-dependent. Chitooligosaccharides with a low molecular weight (2.7,
7.4, and 10.4 Kg mol1) showed the highest activity among all properties tested. These results
suggested that chitosan derivatives from P. henslowii raw material could potentially be used against
phytopathogens or as ingredient in cosmetics and other products related to oxidative stress.
Description
This research was funded by GAC Oeste (PROMAR—European Fisheries Fund) through the
project Pilado add value, under the FP7, grant agreement no. 278612 and by Fundação para a Ciência
e Tecnologia (FCT), through the strategic project UID/MAR/04292/2019 granted to MARE, the Integrated
Programme of SR&TD “Smart Valorization of Endogenous Marine Biological Resources Under a Changing
Climate” (Centro-01-0145-FEDER-000018), co-funded by Centro 2020 programme, Portugal 2020, European Union
through the European Regional Development Fund and the grant awarded to SCM (SFRH/BPD/110400/2015).
Keywords
Polybius henslowii Marine resources Chitosan Chitooligosaccharides Antifungal activity Antioxidant activity
Pedagogical Context
Citation
Avelelas, F., Horta, A., Pinto, L., Cotrim Marques, S., Marques Nunes, P., Pedrosa, R., & Leandro, S. M. (2019). Antifungal and Antioxidant Properties of Chitosan Polymers Obtained from Nontraditional Polybius henslowii Sources. Marine drugs, 17(4), 239. https://doi.org/10.3390/md17040239
Publisher
MDPI