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Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levels

2022Journal article Restricted access
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dc.contributor.authorMorão, Inês F.C.
dc.contributor.authorLemos, Marco F.L.
dc.contributor.authorFélix, Rafael
dc.contributor.authorVieira, Sara
dc.contributor.authorBarata, Carlos
dc.contributor.authorNovais, Sara C.
dc.date.accessioned2022-08-17T14:07:16Z
dc.date.available2022-08-17T14:07:16Z
dc.date.issued2022
dc.descriptionAcknowledgements : This study had the support of Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2020 and the grants awarded to Inês Morão (PD/BD/150562/2019) and Rafael Félix (SFRH/BD/139763/2018). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) cofunded by Centro (2020) program, Portugal 2020, European Union, through the European Regional Development Fund. Sara Novais is funded by national funds (OE), through FCT – Fundação para a Ciência e a Tecnologia, I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. The authors which to acknowledge the support of the Clear Reef Social Fund to grant awarded to Inês Morão. We are thankful to all the members of NGO Programa Tatô and research assistants (biologists Maria Branco and Yedda Oliveira) working at Jalé Beach and to Aurélio Evaristo and João Evaristo that helped us in the field under challenging conditions. We are also thankful to Marta Carmona for the help given in the treatment of the samples for cytology and micronucleus analysis.pt_PT
dc.description.abstractMetals are persistent worldwide being harmful for diverse organisms and having complex and combined effects with other contaminants in the environment. Sea turtles accumulate these contaminants being considered good bioindicator species for marine pollution. However, very little is known on how this is affecting these charismatic animals. Sâo Tomé and Príncipe archipelago harbours important green sea turtle (Chelonia mydas) nesting and feeding grounds. The main goal of this study was to determine metal and metalloid accumulation in the blood of females C. mydas nesting in São Tomé Island, and evaluate the possible impacts of this contamination by addressing molecular stress responses. Gene expression analysis was performed in blood targeting genes involved in detoxification/sequestration and metal transport (mt, mtf and fer), and in antioxidant and oxidative stress responses (cat, sod, gr, tdx, txrd, selp and gclc). Micronuclei analysis in blood was also addressed as a biomarker of genotoxicity. Present results showed significant correlations between different gene expressions with the metals evaluated. The best GLM models and significant relationships were found for mt expression, for which 78% of the variability was attributed to metal levels (Al, Cu, Fe, Hg, Pb and Zn), followed by micronuclei count (65% - Cr, Cu, Fe, Hg, Mn and Zn), tdx expression (52% - Cd, Fe, Mn, Pb and Se), and cat expression (52% - As, Fe, Se and Cd x Hg). Overall, this study demonstrates that these green sea turtles are trying to adapt to the oxidative stress and damage produced by metals through the increased expression of antioxidants and other protectors, which raises concerns about the impacts on these endangered organisms’ fitness. Furthermore, promising biomarker candidates associated to metal stress were identified in this species that may be used in future biomonitoring studies using C. mydas’ blood, allowing for a temporal follow-up of the organisms.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInês F.C. Morão, Marco F.L. Lemos, Rafael Félix, Sara Vieira, Carlos Barata, Sara C. Novais, Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levels, Environmental Pollution, Volume 293, 2022, 118490, ISSN 0269-7491, https://doi.org/10.1016/j.envpol.2021.118490pt_PT
dc.identifier.doi10.1016/j.envpol.2021.118490pt_PT
dc.identifier.issn0269-7491
dc.identifier.urihttp://hdl.handle.net/10400.8/7555
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationUID/MAR/04292/2020pt_PT
dc.relationDesigning a biorefinery for the valorization of invasive seaweed: the case of Sargassum muticum and Asparagopsis armata
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0269749121020728pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBiomarkerspt_PT
dc.subjectGene expressionpt_PT
dc.subjectMarine pollutionpt_PT
dc.subjectMarine turtlespt_PT
dc.subjectOxidative stresspt_PT
dc.titleStress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levelspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDesigning a biorefinery for the valorization of invasive seaweed: the case of Sargassum muticum and Asparagopsis armata
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F150562%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F139763%2F2018/PT
oaire.citation.endPage12pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleEnvironmental Pollutionpt_PT
oaire.citation.volume293pt_PT
oaire.fundingStreamOE
oaire.fundingStreamFARH
person.familyNameMorão
person.familyNameLemos
person.familyNameFaria da Costa Félix
person.familyNameNovais
person.givenNameInês
person.givenNameMarco
person.givenNameRafael Alexandre
person.givenNameSara
person.identifier996337
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person.identifier.ciencia-idBC11-4D98-D13A
person.identifier.ciencia-idA315-5CB0-097C
person.identifier.ciencia-id2216-A2E0-A118
person.identifier.orcid0000-0001-7329-2196
person.identifier.orcid0000-0001-9887-1864
person.identifier.orcid0000-0003-4766-3736
person.identifier.orcid0000-0003-1306-3396
person.identifier.ridF-7951-2011
person.identifier.ridA-9032-2012
person.identifier.scopus-author-id7006042884
person.identifier.scopus-author-id57200377641
person.identifier.scopus-author-id23025463300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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