Compounds altering fat storage in Daphnia magna

Jordão, Rita; Garreta, Elba; Campos, Bruno; Lemos, Marco; Soares, Amadeu M.V.M.; Tauler, Romà; Barata, Carlos

http://hdl.handle.net/10400.8/13235

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
1-s2.0-S0048969715312523-main.pdf		942.15 KB	Adobe PDF	Download

Send Feedback

Authors

Soares, Amadeu M.V.M.

Tauler, Romà

Barata, Carlos

Abstract(s)

The analysis of lipid disruptive effects in invertebrates is limited by our poor knowledge of the lipid metabolic pathways. A recent study showed that tributyltin activated the ecdysteroid, juvenile hormone and retinoic X receptor signaling pathways, and disrupted the dynamics of neutral lipids in the crustacean Daphnia magna impairing the transfer of triacylglycerols to eggs and hence promoting their accumulation in post-spawning females. Tributyltin disruptive effects correlated with lower fitness for offspring and adults. The present study aims to addresses effects of existing compounds on storage lipids in post-spawning females and their health effects. D. magna individuals were exposed 12 chemicals that included vertebrate obesogens (tributyltin, triphenyltin, bisphenol A, nonylphenol, di-2-ethylhexyl phthalate), other contaminants known to affect arthropods (pyriproxyfen, fenarimol, methoprene, emamectin benzoate and fluoxetine), as well as the natural hormones methyl farnesoate and 20-hydroxyecdysone. Reproductive effects were also assessed. Quantitative changes in storage lipids accumulated in lipid droplets were studied using Nile red staining, which showed a close relationship with whole organism levels of triacylglycerols. Ten compounds altered storage lipids in a concentration related manner enhancing (tributyltin, bisphenol A, methyl farnesoate, pyriproxyfen and 20-hydroxyecdysone) or decreasing (nonylphenol, fenarimol, emamectin benzoate, methoprene and fluoxetine) their levels in post-spawning females. Eight compounds that altered lipid levels also had detrimental effects on growth and/or reproduction.

Keywords

Obesogen Lipid disruptor Nuclear receptor Arthropod Reproduction Juvenile receptor

URI

http://hdl.handle.net/10400.8/13235

Citation

Rita Jordão, Elba Garreta, Bruno Campos, Marco F.L. Lemos, Amadeu M.V.M. Soares, Romà Tauler, Carlos Barata, Compounds altering fat storage in Daphnia magna, Science of The Total Environment, Volumes 545–546, 2016, Pages 127-136, ISSN 0048-9697, https://doi.org/10.1016/j.scitotenv.2015.12.097