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Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using nonā€lethal sampling methods

2019Journal article Restricted access
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dc.contributor.authorFerreira, InĆŖs Almeida
dc.contributor.authorCosta, Janina Z.
dc.contributor.authorMacchia, Valeria
dc.contributor.authorThompson, Kimberly Dawn
dc.contributor.authorBaptista, Teresa
dc.date.accessioned2020-07-17T10:45:42Z
dc.date.available2020-07-17T10:45:42Z
dc.date.issued2019
dc.description.abstractOne of the major disease threats affecting the Mediterranean aquaculture industry is viral encephalopathy and retinopathy (VER). The target organs for Betanodavirus detection are the brain and eyes, obtained through lethal sampling. This study aimed to evaluate the efficacy and suitability of nonā€lethal samples for detecting Betanodavirus in European seabass (Dicentrarchus labrax). European seabass juveniles were infected with Betanodavirus, by either an intramuscular injection or immersion (107 TCID50/ml and 106 TCID50/ml, respectively), and samples collected 7, 15 and 30 days postā€infection (dpi). The brain was collected as a lethal sample, and gills, caudal fin and blood as nonā€lethal tissues for detecting Betanodavirus by quantitative reverse transcription PCR (RTā€qPCR). The presence of virus in nonā€lethal tissues was inconsistent, with lower viral loads than in the brain. For blood, higher viral loads were detected in intramuscularā€infected fish at 15 dpi until the end of the challenge. Serum antibodies against Betanodavirus were assessed using an enzymeā€linked immunosorbent assay (ELISA). Antibodies were detected as early as 7 dpi, with higher mean antibody titres at 15 and 30 dpi. The presence of Betanodavirusā€specific antibodies indicates that this is a suitable evaluation method for detecting early stages of the infection.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFerreira, I. A., Costa, J. Z., Macchia, V., Thompson, K.D., & Baptista, T. (2019). Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non-lethal sampling methods. Journal of fish diseases, 42(8), 1097ā€“1105. https://doi.org/10.1111/jfd.13015pt_PT
dc.identifier.doi10.1111/jfd.13015pt_PT
dc.identifier.issn0140-7775
dc.identifier.urihttp://hdl.handle.net/10400.8/5019
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationMARE - Marine and Environmental Sciences Centre
dc.titleDetection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using nonā€lethal sampling methodspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMARE - Marine and Environmental Sciences Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMAR%2F04292%2F2019/PT
oaire.citation.endPage1105pt_PT
oaire.citation.startPage1097pt_PT
oaire.citation.titleJournal of Fish Diseasespt_PT
oaire.citation.volume42pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameBaptista
person.givenNameTeresa
person.identifier.ciencia-idA61F-25C1-034A
person.identifier.orcid0000-0003-1168-7461
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundaĆ§Ć£o para a CiĆŖncia e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery3f0c36c9-c1c4-44e5-801c-b307dbe5625a
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