Development of "in vivo" experimental techniques and modeling methodologies for the evaluation of 4D scaffolds on bone defect in sheep model: an integrative research approach.

Publicações

Effects of Scaffold Electrical Properties on Electric Field Delivery in Bioreactors

Publication . Meneses, João; Fernandes, Sofia R.; Alves, Nuno; Pascoal-Faria, Paula; Miranda, Pedro Cavaleiro

In tissue engineering, cell culture scaffolds have been widely used in combination with electrical stimulation to promote multiple cellular outcomes, like differentiation and proliferation. Nevertheless, the influence of scaffolds on the electric field delivered inside a bioreactor is often ignored and requires a deeper study. By performing numerical analysis in a capacitively coupled setup, this work aimed to predict the effects of the scaffold presence on the electric field, considering multiple combinations of scaffold and culture medium electrical properties. We concluded that the effect of the scaffold on the electric field in the surrounding culture medium was determined by the difference in electrical conductivity of these two materials. The numerical simulations pointed to significant variations in local electric field patterns, which could lead to different cellular outcomes and confound the interpretation of the experimental results.

2021-11Comunicação em conferência

Acesso aberto

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Palavras-chave

Animal model,modelling methodologies,bone defect,4D scaffolds, Agricultural sciences

Entidade financiadora

Fundação para a Ciência e a Tecnologia, I.P.

Programa de financiamento

Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017

Número da atribuição

PTDC/CVT-CVT/31146/2017