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- PCL Scaffolds with Collagen Bioactivator for Applications in Tissue EngineeringPublication . Sousa, Inês; Mendes, Ausenda; Bártolo, Paulo J.The need for human tissues is fundament in certain genetic diseases or accidents of everyday life, they are a primary source for public health. In recent years, to alleviate these effects there was an improvement in the development of tissue engineering, scaffolds or matrices, to support an adequate mechanical and biological environment necessary for the regeneration of damaged tissues. These scaffolds are structures allowing adhesion, differentiation, cell proliferation and the supply of nutrients for growing cells. This paper aims to obtain PCL scaffolds bioactivators, to use as support matrices for the development of human tissues, as PCL is a biomaterial widely used in the biomedical field, due to its biocompatibility, mechanical strength and biodegradability. However, this biomaterial is hydrophobic, which means that there are difficulties in cell adhesion. To solve this problem, a coating of PCL scaffolds with collagen was used, since collagen is a hydrophilic protein existing in the formation of various tissues, so improves cell adhesion.
- Collagen surface modified poly(ε-caprolactone) scaffolds with improved hydrophilicity and cell adhesion propertiesPublication . Sousa, Inês; Mendes, Ausenda; Pereira, Rúben F.; Bártolo, Paulo J.Poly(ε-caprolactone) (PCL)/collagen scaffolds were produced using a four-step procedure comprising (i) the fabrication of PCL scaffolds through an additive bioextrusion system, (ii) the PCL surface modification using a plasma treatment, (iii) the acrylic acid (AAc) grafting by ultraviolet (UV) polymerization, and (iv) the immobilization of collagen into the scaffold surface through the carbodiimide chemistry. This procedure allowed the quick fabrication of highly porous scaffolds with interconnected pores, well-defined internal architecture and improved cell adhesion properties. The immobilization of collagen into the PCL surface led to a significant enhancement in the hydrophilicity, while the biological tests showed a further improvement in the adhesion of fibroblast cells. These results indicate that the collagen surface modification is an effective strategy to render the hydrophobic nature of PCL and overcome the limited cell interaction of 3D PCL scaffolds.