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Browsing by Author "Haute, Gabriela Viegas"

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  • Adjunctive role of Calyptranthes tricona extract with probiotic Kluyveromyces marxianus on colorectal adenocarcinoma Caco-2 cells
    Publication . Kich, Débora Mara; Bitencourt, Shanna; Faleiro, Dalana; Immich, Sheila Mariele; Marmitt, Diorge Jonatas; Baldasso, Tamara; Haute, Gabriela Viegas; Oliveira, Jarbas Rodrigues de; Laufer, Stefan; Pedrosa, Rui; Souza, Claucia Fernanda Volken de; Goettert, Márcia Inês
    The composition of microbiota may influence the development of colorectal cancer (CRC). In addition, probiotics can decrease the chance of developing cancer or its progress. For that reason, it is encouraging to assess the influence of plant extracts as adjuvants of the health-promoting effects of probiotics. Thus, this study aimed to investigate the in vitro beneficial properties of Calyptranthes tricona leaf ethanol extract in association or not with the lactic yeast Kluyveromyces marxianus on colon adenocarcinoma Caco-2 cells. C. tricona extract inhibited the in vitro p38α MAPK activity and exhibited immunomodulation on isolated human lymphocytes. Further, the extract did not induce cytotoxicity towards K. marxianus or Caco-2 cells; leading to an increased yeast adhesion to cells in a dose-dependent manner. In conclusion, this preliminary study demonstrates that C. tricona extract has effects on enzyme inhibition and immune function. In addition, when associated with K. marxianus, C. tricona may possess beneficial properties for application as enhancer of probiotic’s protective role on CRC cells. However, further studies are necessary in order to elucidate the mechanisms involved.
    2019Journal article Restricted access Show more
  • Antimicrobial and antileukemic effects: in vitro activity of Calyptranthes grandifolia aqueous leaf extract
    Publication . Majolo, Fernanda; Bitencourt, Shanna; Monteiro, Bruna Wissmann; Haute, Gabriela Viegas; Alves, Celso; Silva, Joana; Pinteus, Susete; Santos, Roberto Christ Vianna; Torquato, Heron Fernandes Vieira; Paredes-Gamero, Edgar Julian; Oliveira, Jarbas Rodrigues; Souza, Claucia Fernanda Volken De; Pedrosa, Rui; Laufer, Stefan; Goettert, Márcia Inês
    Natural products are still a promising source of bioactive molecules. Food and Drug Administration data showed that approximately 49% of the approved molecules originate naturally or chemicallyresemble these substances, of which more than 70% are being used in anticancer therapy. It is noteworthy that at present there are no scientific studies to prove the effectiveness and safety of a number of plants used in folk medicine such as in the case of Calyptranthes grandifolia O. Berg (Myrtaceae) originally from South America. The aim of the present study was to determine the biological potential and toxicological effects of the aqueous leaf extract of C. grandifolia. The main detected phytoconstituents were condensed tannins and flavonoids and a high quantity of polyphenols. Regarding the antimicrobial potential, the extract exerted inhibitory activity against Pseudomonas aeruginosa. The results also revealed the extract induced DNA damage in a concentration-dependent manner in RAW 264.7 cells. In addition, C. grandifolia produced cytotoxicity in leukemia cell lines (HL60 and Kasumi-1) without affecting isolated human lymphocytes but significantly inhibited JAK3 and p38α enzyme activity. Taken together, these findings add important information on the biological and toxicological effects of C. grandifolia, indicating that aqueous extract may be a source of natural antimicrobial and antileukemic constituents.
    2020Journal article Restricted access Show more