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Chemical Characterization and Bio-Screening of Neuroprotective Potential of Brazilian Brown Seaweed Canistrocarpus cervicornis in 6-OHDA-Induced Neurotoxicity Model

2025-11-25Journal article Open access
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datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg13:Ação Climática
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authordos Santos, Thalisia Cunha
dc.contributor.authorObando, Johana Marcela Concha
dc.contributor.authorSilva, Joana
dc.contributor.authorSantos, Ana Luíza Vidal Pimentel
dc.contributor.authorMartins, Roberto Carlos Campos
dc.contributor.authorCavalcanti, Diana Negrão
dc.contributor.authorPedrosa, Rui
dc.contributor.authorAlves, Celso
dc.date.accessioned2025-12-09T13:07:58Z
dc.date.available2025-12-09T13:07:58Z
dc.date.issued2025-11-25
dc.description.abstractBrazilian native seaweed Canistrocarpus cervicornis (Ochrophyta, Dictyotaceae) is recognized for its chemodiversity, particularly cyclic diterpenes and polysaccharides, yet its relevance to neurological disorders remains unexplored. This study evaluated the neuroprotective potential of a hydroethanolic extract (ECCH), its polar fraction (CCFPol), a dichloromethane extract (ECCD), and eight derived fractions (CCF1–2, CCF3, CCF4, CCF5–6, CCF7, CCF8–10, and CCF11–15). Cytotoxicity was evaluated in SH-SY5Y neuroblastoma cells, and neuroprotection was examined against 6-OHDA–induced toxicity. The mitochondrial membrane potential, ROS and H2O2 generation assays were conducted to explore the mechanisms underlying the observed effects. Among the key findings, the CCF3 fraction exhibited a high content (75.04%) of dolastane-type diterpenoids. Both CCFPol (100 µg/mL) and CCF3 (1 µg/mL) increased cell viability to 68.43 ± 4.60% and 60.61 ± 0.80%, respectively, compared with 6-OHDA–treated cells (50.70 ± 2.71%). Additionally, CCF3 and CCFPol reduced H2O2 levels (200.0 ± 18.19% and 195.5 ± 16.13%, respectively, vs. 6-OHDA-treated cells: 302.2 ± 17.07%) and lowered intracellular ROS (122.6 ± 22.7% and 129.6 ± 19.4%, respectively, vs. 6-OHDA-treated cells: 153.0 ± 32.7%). This is the first study to demonstrate the neuroprotective potential of the C. cervicornis in a 6-OHDA-induced neurotoxicity cellular model, contributing to the understanding of marine bioactive resources and their relevance for neurological research. Additional studies aimed at isolating the active constituents and clarifying their mechanisms of action will further strengthen and expand the biological relevance of this specie as source of neuroprotective agents.eng
dc.description.sponsorshipWe would like to thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for PhD and sandwich doctorate scholarship (TCS), the INCT Nanotechnology for Sustainable Agriculture, the Coordination for the Improvement of Higher Education Personnel–Brazil (MEC-CAPES INCTNanoAgro #888887.986628/2024-00), and the São Paulo Research Foundation (FAPESP) for the technical fellowship TT IV-A [Process 2025/19.448-9] (J.M.C.O).
dc.identifier.citationdos Santos, T.C.; Obando, J.M.C.; Silva, J.; Santos, A.L.V.P.; Martins, R.C.C.; Cavalcanti, D.N.; Pedrosa, R.; Alves, C. Chemical Characterization and Bio-Screening of Neuroprotective Potential of Brazilian Brown Seaweed Canistrocarpus cervicornis in 6-OHDA-Induced Neurotoxicity Model. Antioxidants 2025, 14, 1403. https://doi.org/ 10.3390/antiox14121403
dc.identifier.doi10.3390/antiox14121403
dc.identifier.issn2076-3921
dc.identifier.urihttp://hdl.handle.net/10400.8/14954
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relation.hasversionhttps://www.mdpi.com/2076-3921/14/12/1403
dc.relation.ispartofAntioxidants
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBioproducts
dc.subjectDictyotaceae
dc.subjectNeuroprotection
dc.subjectSH-SY5Y
dc.subjectNeurological diseases
dc.subjectMarine natural products
dc.subjectOxidative stress
dc.subjectMitochondrial disfunction
dc.subjectApoptosis
dc.titleChemical Characterization and Bio-Screening of Neuroprotective Potential of Brazilian Brown Seaweed Canistrocarpus cervicornis in 6-OHDA-Induced Neurotoxicity Modeleng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue12
oaire.citation.titleAntioxidants
oaire.citation.volume14
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamePedrosa
person.familyNameAlves
person.givenNameRui
person.givenNameCelso
person.identifier349272
person.identifier159091
person.identifier.ciencia-id3817-33DE-919E
person.identifier.ciencia-id501C-F268-2E60
person.identifier.orcid0000-0003-0970-0575
person.identifier.orcid0000-0003-1581-2127
person.identifier.ridB-4815-2015
person.identifier.scopus-author-id7005010300
person.identifier.scopus-author-id55654969100
relation.isAuthorOfPublication94496a40-57cf-472f-94e1-5db9d5da78b6
relation.isAuthorOfPublicationba5ab7c2-7474-4ea9-9bf0-ca15fe42809a
relation.isAuthorOfPublication.latestForDiscovery94496a40-57cf-472f-94e1-5db9d5da78b6

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