Publication
Hydrolysis of the phosphoanhydride linkage of cyclic ADP-ribose by the Mn2+-dependent ADP-ribose/CDP-alcohol pyrophosphatase
| dc.contributor.author | Canales, José | |
| dc.contributor.author | Fernández, Ascensión | |
| dc.contributor.author | Rodrigues, Joaquim Rui | |
| dc.contributor.author | Ferreira, Rui | |
| dc.contributor.author | Ribeiro, João Meireles | |
| dc.contributor.author | Cabezas, Alicia | |
| dc.contributor.author | Costas, María Jesús | |
| dc.contributor.author | Cameselle, José Carlos | |
| dc.date.accessioned | 2018-02-12T16:25:15Z | |
| dc.date.available | 2018-02-12T16:25:15Z | |
| dc.date.issued | 2009 | |
| dc.description.abstract | Cyclic ADP-ribose (cADPR) metabolism in mammals is catalyzed by NAD glycohydrolases (NADases) that, besides forming ADP-ribose, form and hydrolyze the N(1)-glycosidic linkage of cADPR. Thus far, no cADPR phosphohydrolase was known. We tested rat ADP-ribose/CDP-alcohol pyrophosphatase (ADPRibase-Mn) and found that cADPR is an ADPRibase-Mn ligand and substrate. ADPRibase-Mn activity on cADPR was 65-fold less efficient than on ADP-ribose, the best substrate. This is similar to the ADP-ribose/cADPR formation ratio by NADases. The product of cADPR phosphohydrolysis by ADPRibase-Mn was N(1)-(5-phosphoribosyl)-AMP, suggesting a novel route for cADPR turnover. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.1016/j.febslet.2009.04.023 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.8/3028 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.subject | Adenosine Diphosphate Ribose | pt_PT |
| dc.subject | Animals | pt_PT |
| dc.subject | Cyclic ADP-Ribose | pt_PT |
| dc.subject | Hydrolysis | pt_PT |
| dc.subject | Manganese | pt_PT |
| dc.subject | Models, Molecular | pt_PT |
| dc.subject | Pyrophosphatases | pt_PT |
| dc.subject | Rats | pt_PT |
| dc.subject | Substrate Specificity | pt_PT |
| dc.title | Hydrolysis of the phosphoanhydride linkage of cyclic ADP-ribose by the Mn2+-dependent ADP-ribose/CDP-alcohol pyrophosphatase | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 1598 | pt_PT |
| oaire.citation.issue | 10 | pt_PT |
| oaire.citation.startPage | 1593 | pt_PT |
| oaire.citation.title | FEBS Letters | pt_PT |
| oaire.citation.volume | 583 | pt_PT |
| person.familyName | Rodrigues | |
| person.givenName | Joaquim Rui | |
| person.identifier.ciencia-id | 9018-0B83-E2C6 | |
| person.identifier.orcid | 0000-0002-9756-1124 | |
| person.identifier.rid | L-4137-2014 | |
| person.identifier.scopus-author-id | 10242931100 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | 52f6ffb2-43e9-4f78-b414-dbac46f80305 | |
| relation.isAuthorOfPublication.latestForDiscovery | 52f6ffb2-43e9-4f78-b414-dbac46f80305 |