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Antioxidant and Cytoprotective Activities of Fucus spiralis Seaweed on a Human Cell in Vitro Model

2017Journal article Open access
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dc.contributor.authorPinteus, Susete
dc.contributor.authorSilva, Joana
dc.contributor.authorAlves, Celso
dc.contributor.authorHorta, André Gustavo Cavadas da
dc.contributor.authorThomas, Olivier P.
dc.contributor.authorPedrosa, Rui
dc.date.accessioned2018-11-12T12:28:59Z
dc.date.available2018-11-12T12:28:59Z
dc.date.issued2017
dc.description.abstractAntioxidants play an important role as Reactive Oxygen Species (ROS) chelating agents and, therefore, the screening for potent antioxidants from natural sources as potential protective agents is of great relevance. The main aim of this study was to obtain antioxidant-enriched fractions from the common seaweed Fucus spiralis and evaluate their activity and efficiency in protecting human cells (MCF-7 cells) on an oxidative stress condition induced by H₂O₂. Five fractions, F1-F5, were obtained by reversed-phase vacuum liquid chromatography. F3, F4 and F5 revealed the highest phlorotannin content, also showing the strongest antioxidant effects. The cell death induced by H₂O₂ was reduced by all fractions following the potency order F4 > F2 > F3 > F5 > F1. Only fraction F4 completely inhibited the H₂O₂ effect. To understand the possible mechanisms of action of these fractions, the cellular production of H₂O₂, the mitochondrial membrane potential and the caspase 9 activity were studied. Fractions F3 and F4 presented the highest reduction on H₂O₂ cell production. All fractions decreased both caspase-9 activity and cell membrane depolarization (except F1). Taken all together, the edible F. spiralis reveal that they provide protection against oxidative stress induced by H₂O₂ on the human MCF-7 cellular model, probably acting as upstream blockers of apoptosis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ijms18020292pt_PT
dc.identifier.other1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.8/3643
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPI AGpt_PT
dc.relationSustainable production of biologically active molecules of marine based origin
dc.relationNeuroprotective effects of molecules isolated from Bifurcaria bifurcata and Codium tomentosum in an in vitro and in vivo Parkinson Disease model.
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/18/2/292pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntioxidantspt_PT
dc.subjectBiological Productspt_PT
dc.subjectCaspase 9pt_PT
dc.subjectFucuspt_PT
dc.subjectHydrogen Peroxidept_PT
dc.subjectMCF-7 Cellspt_PT
dc.subjectMembrane Potential, Mitochondrialpt_PT
dc.subjectOxidative Stresspt_PT
dc.subjectReactive Oxygen Speciespt_PT
dc.subjectSeaweedpt_PT
dc.subjectCytoprotectionpt_PT
dc.titleAntioxidant and Cytoprotective Activities of Fucus spiralis Seaweed on a Human Cell in Vitro Modelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSustainable production of biologically active molecules of marine based origin
oaire.awardTitleNeuroprotective effects of molecules isolated from Bifurcaria bifurcata and Codium tomentosum in an in vitro and in vivo Parkinson Disease model.
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/265896/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F04292%2F2013/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F97764%2F2013/PT
oaire.citation.conferencePlaceBasel, Switzerlandpt_PT
oaire.citation.endPage14pt_PT
oaire.citation.issue2, 292pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume18pt_PT
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oaire.fundingStream9471 - RIDTI
oaire.fundingStreamSFRH
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oaire.fundingStreamSFRH
person.familyNamePinteus
person.familyNameSilva
person.familyNameAlves
person.familyNameHorta
person.familyNamePedrosa
person.givenNameSusete
person.givenNameJoana
person.givenNameCelso
person.givenNameAndré
person.givenNameRui
person.identifier159292
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person.identifier.orcid0000-0003-0970-0575
person.identifier.ridB-3366-2017
person.identifier.ridB-2980-2015
person.identifier.ridB-4815-2015
person.identifier.scopus-author-id55655328300
person.identifier.scopus-author-id56370819800
person.identifier.scopus-author-id55654969100
person.identifier.scopus-author-id55655320600
person.identifier.scopus-author-id7005010300
project.funder.identifierhttp://doi.org/10.13039/501100008530
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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rcaap.typearticlept_PT
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