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Choroidal and retinal structural, cellular and vascular changes in a rat model of Type 2 diabetes

2020-10-15Journal article Open access
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dc.contributor.authorCampos, António
dc.contributor.authorMartins, João
dc.contributor.authorCampos, Elisa J.
dc.contributor.authorSilva, Rufino
dc.contributor.authorAmbrósio, António Francisco
dc.date.accessioned2021-04-23T10:39:16Z
dc.date.available2021-04-23T10:39:16Z
dc.date.issued2020-10-15
dc.description.abstractIncreasing evidence points to inflammation as a key factor in the pathogenesis of diabetic retinopathy (DR). Choroidal changes in diabetes have been reported and several attempts were made to validate in vivo choroidal thickness (CT) as a marker of retinopathy. We aimed to study choroidal and retinal changes associated with retinopathy in an animal model of spontaneous Type 2 diabetes, Goto-Kakizaki (GK) rats. Sclerochoroidal whole mounts and cryosections were prepared from 52-week-old GK and age-matched control Wistar Han rats. CT was measured by optical coherence tomography. Microglia reactivity, pericyte and endothelial cells distribution, and immunoreactivity of vascular endothelial growth factor (VEGF) and VEGF receptor 2 (VEGFR2) were evaluated by immunofluorescence. Choroidal vessels were visualized by direct perfusion with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (Dil). Choroidal vascular density was evaluated by fluorescence microscopy. GK rats had increased CT (58.40 ± 1.15 μm versus 50.90 ± 1.58 μm, p < 0.001), reduced vascular density of the choriocapillaris (CC) (p = 0.045), increased Iba1+ cells density in the outer retina (p = 0.003) and increased VEGFR2 immunoreactivity in most retinal layers (p = 0.021 to 0.037). Choroidal microglial cells and pericytes showed polarity in their distribution, sparing the innermost choroid. This cell-free gap in the inner choroid was more pronounced in GK rats. In summary, GK rats have increased CT with decreased vascular density in the innermost choroid, increased VEGFR2 immunoreactivity in the retina and increased Iba1+ cells density in the outer retina.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.biopha.2020.110811pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.8/5729
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectType 2 diabetespt_PT
dc.subjectChoroidpt_PT
dc.subjectRetinapt_PT
dc.subjectChoroidal thicknesspt_PT
dc.subjectMicrogliapt_PT
dc.subjectVEGFR2pt_PT
dc.titleChoroidal and retinal structural, cellular and vascular changes in a rat model of Type 2 diabetespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage110811pt_PT
oaire.citation.titleBiomedicine & Pharmacotherapypt_PT
oaire.citation.volume132pt_PT
person.familyNameCampos de Figueiredo
person.givenNameAntónio
person.identifier.ciencia-id4F1C-D822-7CFF
person.identifier.orcid0000-0003-0490-5889
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication175bba2d-e9a5-46df-9f0e-da7cb16f04f1
relation.isAuthorOfPublication.latestForDiscovery175bba2d-e9a5-46df-9f0e-da7cb16f04f1

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