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MSCs Conditioned Media and Umbilical Cord Blood Plasma Metabolomics and Composition

2014-11-25Artigo científico Acesso aberto
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datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorPereira, Tiago
dc.contributor.authorIvanova, Galya
dc.contributor.authorCaseiro, Ana Rita
dc.contributor.authorBarbosa, Paula
dc.contributor.authorBártolo, Paulo Jorge
dc.contributor.authorSantos, José Domingos
dc.contributor.authorLuís, Ana Lúcia
dc.contributor.authorMaurício, Ana Colette
dc.contributor.editorIvanovic, Zoran
dc.date.accessioned2026-07-08T15:47:13Z
dc.date.available2026-07-08T15:47:13Z
dc.date.issued2014-11-25
dc.description.abstractHuman mesenchymal stem cells (hMSCs) from umbilical cord (UC) blood (UCB) and matrix are tested clinically for a variety of pathologies but in vitro expansion using culture media containing fetal bovine serum (FBS) is essential to achieve appropriate cell numbers for clinical use. Human UCB plasma (hUCBP) can be used as a supplement for hMSCs culture, since UCB is rich in soluble growth factors and due to worldwide increased number of cryopreserved UCB units in public and private banks, without the disadvantages listed for FBS. On the other hand, the culture media enriched in growth factors produced by these hMSCs in expansion (Conditioned medium - CM) can be an alternative to hMSCs application. The CM of the hMSCs from the UC might be a better therapeutic option compared to cell transplantation, as it can benefit from the local tissue response to the secreted molecules without the difficulties and complications associated to the engraftment of the allo- or xeno-transplanted cells. These facts drove us to know the detailed composition of the hUCBP and CM, by 1H-NMR and Multiplexing LASER Bead Technology. hUCBP is an adequate alternative for the FBS and the CM and hUCBP are important sources of growth factors, which can be used in MSCs-based therapies. Some of the major proliferative, chemotactic and immunomodulatory soluble factors (TGF-β, G-CSF, GM-CSF, MCP-1, IL-6, IL-8) were detected in high concentrations in CM and even higher in hUCBP. The results from 1H-NMR spectroscopic analysis of CM endorsed a better understanding of hMSCs metabolism during in vitro culture, and the relative composition of several metabolites present in CM and hUCBP was obtained. The data reinforces the potential use of hUCBP and CM in tissue regeneration and focus the possible use of hUCBP as a substitute for the FBS used in hMSCs in vitro culture.eng
dc.description.sponsorshipFunding: This research was supported by QREN I&DT Cluster in Development of Products for Regenerative Medicine and Cell Therapies - Projects Biomat & Cell QREN 2008/1372, cofinanced by the European Community FEDER fund through ON2 - O Novo Norte - North Portugal Regional Operational Program 2007–2013, by QREN n˚ 38853/2013 - " Soluções avançadas de regeneração óssea com base em hidrogéis de dextrin - DEXGELERATION", by project "Hybrid Nanostructured Hydrogels: Bone regeneration using Multifunctional injectable Hydrogels - Rebone" - ENMED/0002/2010, and by the program COMPETE - Programa Operacional Factores de Competitividade, Project Pest-OE/AGR/UI0211/2011, and from FCT project PTDC/CVT/103081/2008. The co-author A Almeida is supported by a Post-Doc grant from FCT under the reference SFRH/BPD/79539/2011. The Bruker Avance III HD 600 MHz NMR spectrometer was purchased under the framework of QREN, through project NORTE-07-0162-FEDER-000048, and is part of the Portuguese National NMR Network. The authors would like to state that the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Acknowledgments: The authors would like to gratefully acknowledge the valuable support by of José Manuel Correia Costa, from Laboratório de Parasitologia, Instituto Nacional de Saúde Dr. Ricardo Jorge (INSRJ), Porto, Portugal. ETHICAL APPROVAL: This work is original in that it has not been published before or submitted for publication elsewhere, and will not be submitted elsewhere before a decision has been taken as to its acceptability in this Journal where you are Editor. Each author meets the criteria for authorship and assumes the corresponding responsibility. Umbilical cord blood donations were obtained with written informed consents according to Directive 2004/23/EC which sets the standards of quality and safety for the donation, procurement, testing, processing, preservation, storage and distribution of human tissues and cells.
dc.identifier.citationPereira T, Ivanova G, Caseiro AR, Barbosa P, Bártolo PJ, et al. (2014) MSCs Conditioned Media and Umbilical Cord Blood Plasma Metabolomics and Composition. PLoS ONE 9(11): e113769. doi: https://doi.org/10.1371/journal.pone. 0113769.
dc.identifier.doi10.1371/journal.pone.0113769
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.8/16568
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPublic Library of Science
dc.relationHybrid Nanostuctured Hydrogels: Bone regeneration using multifunctional injectable hydrogels
dc.relationStrategic Project - UI 211 - 2011-2012
dc.relationNew approaches for inactivation, treatment and immunoprophylaxy of cryptosporidiosis
dc.relation.hasversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0113769
dc.relation.ispartofPLoS ONE
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCulture Media
dc.subjectConditioned
dc.subjectFemale
dc.subjectFetal Blood
dc.subjectHumans
dc.subjectInfant
dc.subjectNewborn
dc.subjectMesenchymal Stromal Cells
dc.subjectMetabolomics
dc.subjectPregnancy
dc.titleMSCs Conditioned Media and Umbilical Cord Blood Plasma Metabolomics and Compositioneng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberENMED/0002/2010
oaire.awardNumberPEst-OE/AGR/UI0211/2011
oaire.awardNumberPTDC/CVT/103081/2008
oaire.awardTitleHybrid Nanostuctured Hydrogels: Bone regeneration using multifunctional injectable hydrogels
oaire.awardTitleStrategic Project - UI 211 - 2011-2012
oaire.awardTitleNew approaches for inactivation, treatment and immunoprophylaxy of cryptosporidiosis
oaire.awardURIhttp://hdl.handle.net/10400.8/16236
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FAGR%2FUI0211%2F2011/PT
oaire.awardURIhttp://hdl.handle.net/10400.8/14408
oaire.citation.endPage31
oaire.citation.issue11
oaire.citation.startPage1
oaire.citation.titlePLoS ONE
oaire.citation.volume9
oaire.fundingStreamEuroNanoMed - Second Joint Call 2010
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso para Projectos de I&D em todos os Domínios Científicos - 2008
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCaseiro Santos
person.familyNameBartolo
person.givenNameAna Rita
person.givenNamePaulo
person.identifier203086
person.identifier.ciencia-id5810-9BF9-4522
person.identifier.orcid0000-0002-1143-2461
person.identifier.orcid0000-0003-3683-726X
person.identifier.ridF-2421-2013
person.identifier.scopus-author-id6603353041
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
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Human mesenchymal stem cells (hMSCs) from umbilical cord (UC) blood (UCB) and matrix are tested clinically for a variety of pathologies but in vitro expansion using culture media containing fetal bovine serum (FBS) is essential to achieve appropriate cell numbers for clinical use. Human UCB plasma (hUCBP) can be used as a supplement for hMSCs culture, since UCB is rich in soluble growth factors and due to worldwide increased number of cryopreserved UCB units in public and private banks, without the disadvantages listed for FBS. On the other hand, the culture media enriched in growth factors produced by these hMSCs in expansion (Conditioned medium - CM) can be an alternative to hMSCs application. The CM of the hMSCs from the UC might be a better therapeutic option compared to cell transplantation, as it can benefit from the local tissue response to the secreted molecules without the difficulties and complications associated to the engraftment of the allo- or xeno-transplanted cells. These facts drove us to know the detailed composition of the hUCBP and CM, by 1H-NMR and Multiplexing LASER Bead Technology. hUCBP is an adequate alternative for the FBS and the CM and hUCBP are important sources of growth factors, which can be used in MSCs-based therapies. Some of the major proliferative, chemotactic and immunomodulatory soluble factors (TGF-β, G-CSF, GM-CSF, MCP-1, IL-6, IL-8) were detected in high concentrations in CM and even higher in hUCBP. The results from 1H-NMR spectroscopic analysis of CM endorsed a better understanding of hMSCs metabolism during in vitro culture, and the relative composition of several metabolites present in CM and hUCBP was obtained. The data reinforces the potential use of hUCBP and CM in tissue regeneration and focus the possible use of hUCBP as a substitute for the FBS used in hMSCs in vitro culture.
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