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Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos

2019-11Journal article Restricted access
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dc.contributor.authorSilva, Carla O.
dc.contributor.authorNovais, Sara C.
dc.contributor.authorAlves, Luís M. F.
dc.contributor.authorSoares, Amadeu M. V. M.
dc.contributor.authorBarata, Carlos
dc.contributor.authorLemos, Marco F. L.
dc.date.accessioned2019-10-16T10:36:47Z
dc.date.available2019-10-16T10:36:47Z
dc.date.issued2019-11
dc.date.updated2019-10-07T10:37:11Z
dc.descriptionThis study was funded by Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2013, project MARINE INVADERS – The impact and mechanisms of success of the invasive seaweed Asparagopsis armata on coastal environments (POCI01-0145-FEDER-031144), and the grants awarded to Carla Silva (SFRH/BD/87030/2012), Luís Alves (SFRH/BD/122082/2016) and Sara Novais (SFRH/BPD/94500/2013). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference
dc.description.abstractInhibition of acetylcholinesterase (AChE) activity has been widely used to assess the exposure and effects of anticholinergic environmental contaminants in several species. The aim of this study was to investigate if sublethal concentrations of the organophosphorous pesticide chlorpyrifos (CPF), a well-known AChE inhibitor, would also affect cholinesterases (ChE) in Gibbula umbilicalis and if this inhibition would result in an alteration of its behaviour, in an attempt to link the effects observed at the cellular level with effects at higher levels of ecological relevance. The biochemical properties of ChEs in this species were first characterized through the assessment of different enzymatic forms present in the sea snail, using different substrates and selective inhibitors. The results suggest that G. umbilicalis possess ChEs with characteristics of typical AChE, which should be the main form present. Additionally, in vitro and in vivo effects of CPF on AChE activity were investigated, along with effects on snails' behaviour: the ability of the snails to move/turn after exposure to the contaminant (flipping test). As expected, CPF inhibited AChE activity both in vitro and in vivo conditions. Moreover, the link between AChE activity inhibition and adverse effects on behavioural changes was established: AChE inhibition was positively correlated with the flipping test, indicating a mechanistic relationship between the two endpoints determined in in vivo exposures. This study highlights the importance of linking biochemical endpoints such as AChE activity with higher level endpoints like behavioural alterations, increasing the ecological relevance of the effects observed.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, C. O., Novais, S. C., Alves, L. M. F., Lemos, M. F. L., Soares, A. M. V. M., & Barata, C. (2019). Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos. Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 225. https://doi.org/10.1016/j.cbpc.2019.108570pt_PT
dc.identifier.doi10.1016/j.cbpc.2019.108570
dc.identifier.issn1532-0456
dc.identifier.slugcv-prod-698905
dc.identifier.urihttp://hdl.handle.net/10400.8/4212
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMARINE INVADERS - IMPACT OF THE INVASIVE MACROALGAE ASPARAGOPSIS ARMATA ON MARINE AND COASTAL ENVIRONMENTS.
dc.relationBiomonitoring Atlantic deepwaters through the assessment of biomarkers in sharks
dc.relation.ispartofComparative Biochemistry and Physiology Part C: Toxicology & Pharmacologyen_US
dc.subjectAcetylcholinesterase activitypt_PT
dc.subjectBehaviourpt_PT
dc.subjectCholinesterases characterizationpt_PT
dc.subjectEcotoxicologypt_PT
dc.subjectMarine snailspt_PT
dc.titleLinking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifospt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMARINE INVADERS - IMPACT OF THE INVASIVE MACROALGAE ASPARAGOPSIS ARMATA ON MARINE AND COASTAL ENVIRONMENTS.
oaire.awardTitleBiomonitoring Atlantic deepwaters through the assessment of biomarkers in sharks
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F04292%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F87030%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F122082%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F94500%2F2013/PT
oaire.citation.startPage108570pt_PT
oaire.citation.titleComparative Biochemistry and Physiology - Part C: Toxicology and Pharmacologypt_PT
oaire.citation.volume225pt_PT
oaire.fundingStream5876
oaire.fundingStreamPOR_CENTRO
oaire.fundingStreamSFRH
person.familyNameSilva
person.familyNameNovais
person.familyNameAlves
person.familyNameLemos
person.givenNameCarla
person.givenNameSara
person.givenNameLuís
person.givenNameMarco
person.identifier996337
person.identifier.ciencia-id771A-3CFD-5F3E
person.identifier.ciencia-id2216-A2E0-A118
person.identifier.ciencia-idB615-BAFB-B8ED
person.identifier.orcid0000-0002-4613-1132
person.identifier.orcid0000-0003-1306-3396
person.identifier.orcid0000-0001-5106-7430
person.identifier.orcid0000-0001-9887-1864
person.identifier.ridA-9032-2012
person.identifier.ridF-7951-2011
person.identifier.scopus-author-id57191570330
person.identifier.scopus-author-id23025463300
person.identifier.scopus-author-id7006042884
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid971F-ACCA-C0D1 | Marco Lemos
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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