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Antioxidant and antimicrobial potential of the Bifurcaria bifurcata epiphytic bacteria

2014Journal article Open access
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dc.contributor.authorHorta, André
dc.contributor.authorPinteus, Susete
dc.contributor.authorAlves, Celso
dc.contributor.authorFino, Nádia
dc.contributor.authorSilva, Joana
dc.contributor.authorFernandez, Sara
dc.contributor.authorRodrigues, Américo
dc.contributor.authorPedrosa, Rui
dc.date.accessioned2021-08-12T12:25:44Z
dc.date.available2021-08-12T12:25:44Z
dc.date.issued2014
dc.description.abstractSurface-associated marine bacteria are an interesting source of new secondary metabolites. The aim of this study was the isolation and identification of epiphytic bacteria from the marine brown alga, Bifurcaria bifurcata, and the evaluation of the antioxidant and antimicrobial activity of bacteria extracts. The identification of epiphytic bacteria was determined by 16S rRNA gene sequencing. Bacteria extracts were obtained with methanol and dichloromethane (1:1) extraction. The antioxidant activity of extracts was performed by quantification of total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and oxygen radical absorbance capacity (ORAC). Antimicrobial activities were evaluated against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Salmonella enteritidis, Staphylococcus aureus, Saccharomyces cerevisiae and Candida albicans. A total of 39 Bifurcaria bifurcata-associated bacteria were isolated and 33 were identified as Vibrio sp. (48.72%), Alteromonas sp. (12.82%), Shewanella sp. (12.26%), Serratia sp. (2.56%), Citricoccus sp. (2.56%), Cellulophaga sp. (2.56%), Ruegeria sp. (2.56%) and Staphylococcus sp. (2.56%). Six (15.38%) of the 39 bacteria Bifurcaria bifurcata-associated bacteria presented less than a 90% Basic Local Alignment Search Tool (BLAST) match, and some of those could be new. The highest antioxidant activity and antimicrobial activity (against B. subtilis) was exhibited by strain 16 (Shewanella sp.). Several strains also presented high antimicrobial activity against S. aureus, mainly belonging to Alteromonas sp. and Vibrio sp. There were no positive results against fungi and Gram-negative bacteria. Bifurcaria bifurcata epiphytic bacteria were revealed to be excellent sources of natural antioxidant and antimicrobial compounds.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHorta A, Pinteus S, Alves C, Fino N, Silva J, Fernandez S, Rodrigues A, Pedrosa R. Antioxidant and antimicrobial potential of the Bifurcaria bifurcata epiphytic bacteria. Mar Drugs. 2014 Mar 24;12(3):1676-89. doi: 10.3390/md12031676. PMID: 24663118; PMCID: PMC3967231.pt_PT
dc.identifier.doidoi:10.3390/md12031676pt_PT
dc.identifier.issn1660-3397
dc.identifier.urihttp://hdl.handle.net/10400.8/6085
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationSustainable production of biologically active molecules of marine based origin
dc.subjectMarine microorganismspt_PT
dc.subjectNatural compoundspt_PT
dc.subjectHuman pathogenicpt_PT
dc.subjectAlteromonas sppt_PT
dc.subjectShewanella sppt_PT
dc.subjectAntibacterialpt_PT
dc.subjectSurface-associated microorganismspt_PT
dc.subjectMarine symbiosispt_PT
dc.subjectMacro-algaept_PT
dc.subjectSeaweedspt_PT
dc.titleAntioxidant and antimicrobial potential of the Bifurcaria bifurcata epiphytic bacteriapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSustainable production of biologically active molecules of marine based origin
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/265896/EU
oaire.citation.endPage1689pt_PT
oaire.citation.startPage1676pt_PT
oaire.citation.titleMarine drugspt_PT
oaire.citation.volume12pt_PT
oaire.fundingStreamFP7
person.familyNameHorta
person.familyNamePinteus
person.familyNameAlves
person.familyNameSilva
person.familyNameRodrigues
person.familyNamePedrosa
person.givenNameAndré
person.givenNameSusete
person.givenNameCelso
person.givenNameJoana
person.givenNameAmérico
person.givenNameRui
person.identifier159292
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person.identifier.orcid0000-0003-1581-2127
person.identifier.orcid0000-0003-1224-1699
person.identifier.orcid0000-0002-5293-5583
person.identifier.orcid0000-0003-0970-0575
person.identifier.ridB-2980-2015
person.identifier.ridB-3366-2017
person.identifier.ridF-9573-2010
person.identifier.ridB-4815-2015
person.identifier.scopus-author-id55655320600
person.identifier.scopus-author-id55655328300
person.identifier.scopus-author-id55654969100
person.identifier.scopus-author-id56370819800
person.identifier.scopus-author-id15745701600
person.identifier.scopus-author-id7005010300
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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