Publicação
High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain
| dc.contributor.author | Samhan-Arias, A. K. | |
| dc.contributor.author | López-Sánchez, C. | |
| dc.contributor.author | Marques-da-Silva, D. | |
| dc.contributor.author | Lagoa, Ricardo | |
| dc.contributor.author | Garcia-Lopez, V. | |
| dc.contributor.author | García-Martínez, V. | |
| dc.contributor.author | Gutierrez-Merino, C. | |
| dc.date.accessioned | 2025-06-04T10:23:24Z | |
| dc.date.available | 2025-06-04T10:23:24Z | |
| dc.date.issued | 2015-04-08 | |
| dc.description.abstract | Cytochrome b5 reductase (Cb5R) and cytochrome b5 (Cb5) form an enzymatic redox system that plays many roles in mammalian cells. In the last 15 years, it has been proposed that this system is involved in the recycling of ascorbate, a vital antioxidant molecule in the brain and that its deregulation can lead to the production of reactive oxygen species that play a major role in oxidativeinduced neuronal death. In this work, we have performed a regional and cellular distribution study of the expression of this redox system in adult rat brain by anti-Cb5R isoform 3 and anti-Cb5 antibodies. We found high expression levels in cerebellar cortex, labeling heavily granule neurons and Purkinje cells, and in structures such as the fastigial, interposed and dentate cerebellar nuclei. A large part of Cb5R isoform 3 in the cerebellum cortex was regionalized in close proximity to the lipid raft-like nanodomains, labeled with cholera toxin B, as we have shown by fluorescence resonance energy transfer imaging. In addition, vestibular, reticular and motor nuclei located at the brain stem level and pyramidal neurons of somatomotor areas of the brain cortex and of the hippocampus have been also found to display high expression levels of these proteins. All these results point out the enrichment of Cb5R isoform 3/Cb5 system in neuronal cells and structures of the cerebellum and brain stem whose functional impairment can account for neurological deficits reported in type II congenital methemoglobinemia, as well as in brain areas highly prone to undergo oxidative stress-induced neurodegeneration. | eng |
| dc.description.sponsorship | AKSA was recipient of a post-doctoral fellowship of the Junta de Extremadura and DMS was recipient of a predoctoral fellowship of the Spanish Ministerios de Ciencia e Innovación y Economía y Competitividad. The technical help of Dr. José Antonio Tapia with confocal microscopy images acquisition is gratefully acknowledged. This work has been financed with research project BFU2011-30178 of the Spanish Ministerio de Economía y Competitividad and grants to research groups CCV008/BBB008 and CTS005 of the Plan Regional de I + D + I of the Junta/Gobierno de Extremadura (Spain). These fellowships and grants have been cofinanced with the European Fund for Economic and Regional Development (FEDER). | |
| dc.identifier.citation | Samhan-Arias, A.K., López-Sánchez, C., Marques-da-Silva, D. et al. High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain. Brain Struct Funct 221, 2147–2162 (2016). https://doi.org/10.1007/s00429-015-1036-5 | |
| dc.identifier.doi | 10.1007/s00429-015-1036-5 | |
| dc.identifier.issn | 1863-2653 | |
| dc.identifier.issn | 1863-2661 | |
| dc.identifier.uri | http://hdl.handle.net/10400.8/13095 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Springer Science and Business Media LLC | |
| dc.relation.hasversion | https://link.springer.com/article/10.1007/s00429-015-1036-5 | |
| dc.relation.ispartof | Brain Structure and Function | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Cytochrome b5 | |
| dc.subject | Cytochrome b5 reductase isoform 3 | |
| dc.subject | Rat brain | |
| dc.subject | Cerebellum | |
| dc.subject | Brain stem | |
| dc.subject | Purkinje neurons | |
| dc.subject | Cerebellar granule neurons | |
| dc.subject | Cerebellar and vestibular nuclei | |
| dc.subject | Hippocampus | |
| dc.subject | Neocortex | |
| dc.title | High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 2162 | |
| oaire.citation.issue | 4 | |
| oaire.citation.startPage | 2147 | |
| oaire.citation.title | Brain Structure and Function | |
| oaire.citation.volume | 221 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Lagoa | |
| person.givenName | Ricardo | |
| person.identifier | 124357 | |
| person.identifier.ciencia-id | 5C18-A29A-44AB | |
| person.identifier.orcid | 0000-0003-2375-6612 | |
| person.identifier.scopus-author-id | 23051352300 | |
| relation.isAuthorOfPublication | 8a139213-9a89-4bd3-93ee-1e332519f96b | |
| relation.isAuthorOfPublication.latestForDiscovery | 8a139213-9a89-4bd3-93ee-1e332519f96b |