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High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain
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Orientador(es)
Resumo(s)
Cytochrome b5 reductase (Cb5R) and cytochrome b5 (Cb5) form an enzymatic redox system that plays many roles in mammalian cells. In the last 15 years,
it has been proposed that this system is involved in the recycling of ascorbate, a vital antioxidant molecule in the brain and that its deregulation can lead to the production of reactive oxygen species that play a major role in oxidativeinduced neuronal death. In this work, we have performed a regional and cellular distribution study of the expression of this redox system in adult rat brain by anti-Cb5R isoform 3 and anti-Cb5 antibodies. We found high expression levels
in cerebellar cortex, labeling heavily granule neurons and Purkinje cells, and in structures such as the fastigial, interposed and dentate cerebellar nuclei. A large part of Cb5R isoform 3 in the cerebellum cortex was regionalized in close proximity to the lipid raft-like nanodomains, labeled with cholera toxin B, as we have shown by fluorescence resonance energy transfer imaging. In addition, vestibular, reticular and motor nuclei located at the brain stem level and pyramidal neurons of somatomotor areas of the brain cortex and of the hippocampus have been also found to display high expression levels of these proteins.
All these results point out the enrichment of Cb5R isoform 3/Cb5 system in neuronal cells and structures of the cerebellum and brain stem whose functional impairment can account for neurological deficits reported in type II congenital methemoglobinemia, as well as in brain areas highly prone to undergo oxidative stress-induced neurodegeneration.
Descrição
Palavras-chave
Cytochrome b5 Cytochrome b5 reductase isoform 3 Rat brain Cerebellum Brain stem Purkinje neurons Cerebellar granule neurons Cerebellar and vestibular nuclei Hippocampus Neocortex
Contexto Educativo
Citação
Samhan-Arias, A.K., López-Sánchez, C., Marques-da-Silva, D. et al. High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain. Brain Struct Funct 221, 2147–2162 (2016). https://doi.org/10.1007/s00429-015-1036-5
Editora
Springer Science and Business Media LLC