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Fluoxetine arrests growth of the model diatom Phaeodactylum tricornutum by increasing oxidative stress and altering energetic and lipid metabolism

2020Journal article Open access
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dc.contributor.authorFeijão, Eduardo
dc.contributor.authorCarvalho, Ricardo Cruz de
dc.contributor.authorDuarte, Irina A.
dc.contributor.authorMatos, Ana Rita
dc.contributor.authorCabrita, Maria Teresa
dc.contributor.authorNovais, Sara C.
dc.contributor.authorLemos, Marco F.L.
dc.contributor.authorCaçador, Isabel
dc.contributor.authorMarques, João Carlos
dc.contributor.authorReis-Santos, Patrick
dc.contributor.authorFonseca, Vanessa F.
dc.contributor.authorDuarte, Bernardo
dc.date.accessioned2021-08-11T12:05:56Z
dc.date.available2021-08-11T12:05:56Z
dc.date.issued2020
dc.descriptionFUNDING : The authors would like to thank Fundação para a Ciência e a Tecnologia (FCT) for funding the research via project grants PTDC/MAR-EST/3048/2014 (BIOPHARMA), PTDC/CTA-AMB/30056/2017 (OPTOX), UIDB/04292/2020, and UID/MULTI/04046/2013. Work was also funded by the Integrated Programme of SR&TD SmartBioR (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 program, Portugal 2020, European Union, through the European Regional Development Fund. BD and VF were supported by investigation contracts (CEECIND/00511/2017 and DL57/2016/CP1479/CT0024). PR-S was supported by FCT through a postdoctoral grant (SFRH/BPD/95784/2013). ID was supported by a Ph.D. grant (SFRH/BD/138376/2018). MC acknowledges the FCT and IGOT (contract under the DL 57/2016 and L57/2017 Program).
dc.description.abstractPharmaceutical residues impose a new and emerging threat to aquatic environments and its biota. One of the most commonly prescribed pharmaceuticals is the antidepressant fluoxetine, a selective serotonin re-uptake inhibitor that has been frequently detected, in concentrations up to 40 ug L-1, in aquatic ecosystems. The present study aims to investigate the ecotoxicity of fluoxetine at environmentally relevant concentrations (0.3, 0.6, 20, 40, and 80 ug L-1) on cell energy and lipid metabolism, as well as oxidative stress biomarkers in the model diatom Phaeodactylum tricornutum. Exposure to higher concentrations of fluoxetine negatively affected cell density and photosynthesis through a decrease in the active PSII reaction centers. Stress response mechanisms, like b-carotene (b-car) production and antioxidant enzymes [superoxide dismutase (SOD) and ascorbate peroxidase (APX)] up-regulation were triggered, likely as a positive feedback mechanism toward formation of fluoxetine-induced reactive oxygen species. Lipid peroxidation products increased greatly at the highest fluoxetine concentration whereas no variation in the relative amounts of long chain polyunsaturated fatty acids (LC-PUFAs) was observed. However, monogalactosyldiacylglycerol-characteristic fatty acids such as C16:2 and C16:3 increased, suggesting an interaction between light harvesting pigments, lipid environment, and photosynthesis stabilization. Using a canonical multivariate analysis, it was possible to evaluate the efficiency of the application of bio-optical and biochemical techniques as potential fluoxetine exposure biomarkers in P. tricornutum. An overall classification efficiency to the different levels of fluoxetine exposure of 61.1 and 88.9% were obtained for bio-optical and fatty acids profiles, respectively, with different resolution degrees highlighting these parameters as potential efficient biomarkers. Additionally, the negative impact of this pharmaceutical molecule on the primary productivity is also evident alongside with an increase in respiratory oxygen consumption. From the ecological point of view, reduction in diatom biomass due to continued exposure to fluoxetine may severely impact estuarine and coastal trophic webs, by both a reduction in oxygen primary productivity and reduced availability of key fatty acids to the dependent heterotrophic upper levels.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFeijão E, Cruz de Carvalho R, Duarte IA, Matos AR, Cabrita MT, Novais SC, Lemos MFL, Caçador I, Marques JC, Reis-Santos P, Fonseca VF and Duarte B (2020) Fluoxetine Arrests Growth of the Model Diatom Phaeodactylum tricornutum by Increasing Oxidative Stress and Altering Energetic and Lipid Metabolism. Front. Microbiol. 11:1803. doi: 10.3389/fmicb.2020.01803pt_PT
dc.identifier.doi10.3389/fmicb.2020.01803pt_PT
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10400.8/6076
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontierspt_PT
dc.relationDevelopment and validation of bio-optical ecotoxicological tests in marine phototrophs.
dc.relationMarine and Environmental Sciences Centre
dc.relationBiosystems & Integrative Sciences Institute
dc.relationNot Available
dc.relationBIOCONNECT - CONECTIVIDADE EM POPULAÇÕES DE PEIXES ESTUARINOS E COSTEIROS: MARCADORES NATURAIS COMO UM INSTRUMENTO PARA GESTÃO E CONSERVAÇÃO
dc.relationPharmaceuticals in the environment: occurrence and exposure effects in non-target fish species
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fmicb.2020.01803/fullpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPharmaceuticalspt_PT
dc.subjectAntidepressantpt_PT
dc.subjectMicroalgaept_PT
dc.subjectEcotoxicitypt_PT
dc.subjectPhotobiologypt_PT
dc.subjectCell energypt_PT
dc.subjectBiomarkerspt_PT
dc.subjectFatty acid profilept_PT
dc.titleFluoxetine arrests growth of the model diatom Phaeodactylum tricornutum by increasing oxidative stress and altering energetic and lipid metabolismpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDevelopment and validation of bio-optical ecotoxicological tests in marine phototrophs.
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleBiosystems & Integrative Sciences Institute
oaire.awardTitleNot Available
oaire.awardTitleBIOCONNECT - CONECTIVIDADE EM POPULAÇÕES DE PEIXES ESTUARINOS E COSTEIROS: MARCADORES NATURAIS COMO UM INSTRUMENTO PARA GESTÃO E CONSERVAÇÃO
oaire.awardTitlePharmaceuticals in the environment: occurrence and exposure effects in non-target fish species
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR-EST%2F3048%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTA-AMB%2F30056%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04046%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F00511%2F2017%2FCP1387%2FCT0039/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBPD%2F95784%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F138376%2F2018/PT
oaire.citation.titleFrontiers in Microbiologypt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 2017
oaire.fundingStreamOE
person.familyNameNovais
person.familyNameLemos
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