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RGA & GAI: On the characterization of two DELLA plant growth repressor proteins

2018Conference object Open access
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dc.contributor.authorVaz, Daniela C.
dc.contributor.authorSousa, Raquel
dc.contributor.authorSimões, Marco
dc.contributor.authorRodrigues, Américo
dc.date.accessioned2019-04-16T08:54:38Z
dc.date.available2019-04-16T08:54:38Z
dc.date.issued2018
dc.description.abstractDELLA proteins are a family of nuclear proteins responsible for plant growth modulation. They act as growth repressor proteins in response to gibberellin signaling pathways. Five DELLA protein homologs were found in Arabidopsis thaliana, namely, RGA (Repressor of Gibberellic Acid), GAI (Gibberellic Acid Insensitive) and three RGA-like proteins (RGL-1, RGL-2 and RGL-3). The RGA- DELLA and GAI-DELLA protein homologs have been classified as Intrinsically Unstructured Proteins (IUPs) that undergo a disorder-to-order transition upon receptor binding. This structural change has found to be physiologically relevant for biological signaling and molecular recognition. Thus, in order to better characterize the structural features and molecular changes that govern these conformational variations of the polypeptide chain, we have produced recombinant RGADELLA and GAI-DELLA proteins in three length-versions, i.e. full-length, N-terminal and C-terminal versions. Full-length and terminal versions present different sequence motifs, attributed to different biological functions. All proteins were analyzed spectro-photometrically, via Light Scattering (LS), Circular Dichroism (CD) and intrinsic and extrinsic fluorescence (ANS binding), in order to compare spectral profiles, secondary structure propensities, levels of solvent exposure and structural compactness. Full-length and terminal variants exhibit different behaviors, spectral profiles and levels of compactness that can be related to different protein domains and ultimately to different functional implications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.4172/0974-276X-C3-119pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.8/3918
dc.language.isoengpt_PT
dc.peerreviewednopt_PT
dc.titleRGA & GAI: On the characterization of two DELLA plant growth repressor proteinspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.startPage37pt_PT
oaire.citation.titleJournal of Proteomics and Bioinformaticspt_PT
oaire.citation.volume11pt_PT
person.familyNameBarroso de Moura Cipreste Vaz
person.familyNameDias Simões
person.familyNameRodrigues
person.givenNameDaniela
person.givenNameMarco Aurélio
person.givenNameAmérico
person.identifier.ciencia-id801A-7761-328C
person.identifier.ciencia-id1D1C-C217-F68A
person.identifier.orcid0000-0001-7562-4676
person.identifier.orcid0000-0001-7995-7304
person.identifier.orcid0000-0002-5293-5583
person.identifier.ridR-5243-2017
person.identifier.ridF-9573-2010
person.identifier.scopus-author-id6602838931
person.identifier.scopus-author-id15745701600
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication518f12af-3297-4334-b00b-c06e17b2cf27
relation.isAuthorOfPublicatione2a25105-22d0-4e1d-9e51-2befd64f1baf
relation.isAuthorOfPublication718ad6e8-1d3a-4ce3-9870-e4ed6732c30b
relation.isAuthorOfPublication.latestForDiscovery718ad6e8-1d3a-4ce3-9870-e4ed6732c30b

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