Publication
Immobilized heterologous Rhizopus oryzae lipase: A feasible biocatalyst for the production of human milk fat substitutes
| datacite.subject.sdg | 02:Erradicar a Fome | |
| datacite.subject.sdg | 08:Trabalho Digno e Crescimento Económico | |
| datacite.subject.sdg | 15:Proteger a Vida Terrestre | |
| dc.contributor.author | Tecelão, Carla | |
| dc.contributor.author | Guillén, Marina | |
| dc.contributor.author | Valero, Francisco | |
| dc.contributor.author | Ferreira-Dias, Suzana | |
| dc.date.accessioned | 2025-11-26T10:45:23Z | |
| dc.date.available | 2025-11-26T10:45:23Z | |
| dc.date.issued | 2012-08 | |
| dc.description.abstract | This work aims at evaluating the potentialities of a heterologous Rhizopus oryzae lipase (rROL) as a feasible biocatalyst, to be used as an alternative to commercial lipases, for the synthesis of Human Milk Fat Substitutes (HMFS). This enzyme was immobilized in three different supports (Accurel® MP 1000, Eupergit® C and Lewatit® VP OC 1600) and tested as catalysts for the acidolysis between tripalmitin and oleic acid, batchwise, at 60 ◦C, in solvent-free medium. The highest molar incorporations of oleic acid in the triacylglycerols were achieved with rROL in Lewatit® VP OC 1600 and Accurel® MP 1000, although the highest immobilization yields were observed with Eupergit® C. Quasi-equilibrium was attained after 6 h or 12 h reaction, with about 30 mol.% and 22 mol.% of oleic acid incorporation, when rROL immobilized in Lewatit and Accurel were used, respectively. Also, 23 h batch operational stability tests were performed: half-life times (t1/2) of 34.5 h and 64.0 h were achieved for rROL immobilized in Accurel® MP 1000 and in Lewatit® VP OC 1600, respectively. An important increase in the operational stability was observed for rROL immobilized in Lewatit after rehydration at the end of each batch (t1/2 = 202 h). Thus, the loss of activity may be explained by a progressive dehydration occurring along the reaction. | eng |
| dc.description.sponsorship | The authors are grateful to the Fundação para a Ciência e a Tecnologia (FCT), Portugal, for a PhD fellowship for Mrs. Carla Tecelão (SFRH/BD/45773/2008). This study was supported by the: (i) Program CYTED, under the scope of the “Ibero-American Network for the Extraction and Enzymatic Transformation of Functional Ingredients and Nutraceuticals from Regional Plants and Agro-Residues (ENZNUT)”, (ii) by the project CTQ2010-15131 of the Spanish Ministry of Science and Innovation, (iii) by 2009-SGR-281 and Reference Network in Biotechnology (XRB) (Generalitat de Catalunya) and by (iv) the Integrated Action Portugal-Spain E-29/11. | |
| dc.identifier.citation | Carla Tecelão, Marina Guillén, Francisco Valero, Suzana Ferreira-Dias, Immobilized heterologous Rhizopus oryzae lipase: A feasible biocatalyst for the production of human milk fat substitutes, Biochemical Engineering Journal, Volume 67, 2012, Pages 104-110, ISSN 1369-703X, https://doi.org/10.1016/j.bej.2012.06.001. | |
| dc.identifier.doi | 10.1016/j.bej.2012.06.001 | |
| dc.identifier.issn | 1369-703X | |
| dc.identifier.uri | http://hdl.handle.net/10400.8/14729 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Elsevier | |
| dc.relation | SFRH/BD/45773/2008 | |
| dc.relation.hasversion | https://www.sciencedirect.com/science/article/pii/S1369703X12001714 | |
| dc.relation.ispartof | Biochemical Engineering Journal | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Acidolysis | |
| dc.subject | Human milk fat substitutes | |
| dc.subject | Immobilization | |
| dc.subject | Recombinant lipase | |
| dc.subject | Rhizopus oryzae | |
| dc.subject | Operational stability | |
| dc.title | Immobilized heterologous Rhizopus oryzae lipase: A feasible biocatalyst for the production of human milk fat substitutes | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 110 | |
| oaire.citation.startPage | 104 | |
| oaire.citation.title | Biochemical Engineering Journal | |
| oaire.citation.volume | 67 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Tecelão | |
| person.givenName | Carla | |
| person.identifier.ciencia-id | 9B1D-4AC0-1B03 | |
| person.identifier.orcid | 0000-0003-2423-0495 | |
| person.identifier.scopus-author-id | 7801371939 | |
| relation.isAuthorOfPublication | 0d890808-4e69-4e6c-a631-802f1a7dce1f | |
| relation.isAuthorOfPublication.latestForDiscovery | 0d890808-4e69-4e6c-a631-802f1a7dce1f |