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Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regeneration

2019Journal article Open access
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dc.contributor.authorSerôdio, Ricardo
dc.contributor.authorSchickert, Sónia L.
dc.contributor.authorCosta-Pinto, Ana R.
dc.contributor.authorDias, Juliana R.
dc.contributor.authorGranja, Pedro L.
dc.contributor.authorYang, Fang
dc.contributor.authorOliveira, Ana L.
dc.date.accessioned2023-06-05T13:46:45Z
dc.date.available2023-06-05T13:46:45Z
dc.date.issued2019
dc.descriptionThe authors acknowledge Portuguese National Funds from FCT - Fundação para a Ciência e a Tecnologia through project UID/Multi/50016/2013; Program FCT Investigator to A.L. Oliveira (IF/00411/2013), project SERICAMED (IF/00411/2013/CP1167), and through “Biotherapies-Bioengineered Therapies for Infectious Diseases and Tissue Regeneration”, funded by Norte2020. Project “IBEROS” (0245_IBEROS_1_E), funded by Fundo Europeu de Desenvolvimento Regional in the frame of Programa Interreg V A Espanha - Portugal (POCTEP) 2014–2020.pt_PT
dc.description.abstractIn this study, silk fibroin (SF)/poly(ethylene oxide) (PEO) membranes were designed and fabricated by combining ultrasound sonication prior to electrospinning (0 to 20 min) as a strategy to physically control the rheological properties of solutions (10 to 30% w/v PEO) and to improve the spinnability of the system. PEO has proved to be essential as a co-spinning agent to assure good membrane reproducibility and enough flexibility for clinical manipulation. The rheological tests indicated that sonication greatly increased the viscosity of SF/PEO solutions and further enhanced the quality of the produced electrospun fibers with consequent improved mechanical properties in dry and wet conditions. By tuning the viscosity of the solutions using a simple sonication step prior to electrospinning, it was possible to induce water stability in the as-electrospun matrix, as demonstrated by infra-red spectroscopy. This reduced complexity in the process since it was not necessary to concentrate silk prior to electrospinning while avoiding the use of toxic solvents to perform a post-processing stabilization treatment which usually causes dimensional changes to the SF materials. Sonication pre-treatment allowed for minimizing the amount of synthetic polymer used to achieve the desirable mechanical properties (with the modulus ranging between 90 and 170 MPa), while avoiding a further water stabilization treatment. It also had a positive impact in the in vitro cell behavior of human primary periodontal ligament cells (hPDLs), resulting in a marked increase in cell proliferation. The present developed work constitutes a step forward towards simplicity and a better fabrication control of viable electrospun SFbased membranes for periodontal regeneration.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRicardo Serôdio, Sónia L. Schickert, Ana R. Costa-Pinto, Juliana R. Dias, Pedro L. Granja, Fang Yang, Ana L. Oliveira, Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regeneration, Materials Science and Engineering: C, Volume 98, 2019, Pages 969-981, ISSN 0928-4931, https://doi.org/10.1016/j.msec.2019.01.055.pt_PT
dc.identifier.doihttps://doi.org/10.1016/j.msec.2019.01.055pt_PT
dc.identifier.issn0928-4931
dc.identifier.pmid30813104
dc.identifier.urihttp://hdl.handle.net/10400.8/8563
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier - ScienceDirectpt_PT
dc.relationCentre for Biotechnology and Fine Chemistry
dc.relationSERICAMED - Designing Functional Silk-Based Biomaterials for In Situ Periodontal Regeneration.
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0928493118313675?casa_token=6kUoz3JHCc8AAAAA:HU4Bl6IFVbbefspU-_JfDDzHOIw9iZC3hOuHsihcyEkT0o2ojUqE7zhjT2QxxtTAnb64HL95pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSilk fibroinpt_PT
dc.subjectPoly(ethylene oxide) membranept_PT
dc.subjectPeriodontal regenerationpt_PT
dc.subjectElectrospinningpt_PT
dc.subjectUltrasound sonicationpt_PT
dc.titleUltrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regenerationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Biotechnology and Fine Chemistry
oaire.awardTitleSERICAMED - Designing Functional Silk-Based Biomaterials for In Situ Periodontal Regeneration.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00411%2F2013%2FCP1167%2FCT0001/PT
oaire.citation.endPage981pt_PT
oaire.citation.startPage969pt_PT
oaire.citation.titleMaterials Science and Engineering: Cpt_PT
oaire.citation.volume98pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamInvestigador FCT
person.familyNameDias
person.familyNameGranja
person.givenNameJuliana
person.givenNamePedro
person.identifier633669
person.identifier1017757
person.identifier.ciencia-id0612-D971-F00D
person.identifier.ciencia-idDB1F-B9B7-E4FD
person.identifier.orcid0000-0002-7079-1069
person.identifier.orcid0000-0003-2761-4929
person.identifier.ridA-6086-2014
person.identifier.ridB-1685-2008
person.identifier.scopus-author-id55749822200
person.identifier.scopus-author-id6602391346
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication4210655d-5d58-4736-8bfb-a7bf19849aae
relation.isAuthorOfPublication.latestForDiscovery9e70a657-1b71-4366-ab59-b9f988a19d5a
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