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Neuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma model

2018Journal article Open access
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dc.contributor.authorSilva, Joana
dc.contributor.authorAlves, Celso
dc.contributor.authorPinteus, Susete
dc.contributor.authorMendes, Susana
dc.contributor.authorPedrosa, Rui
dc.date.accessioned2020-07-06T14:27:03Z
dc.date.available2020-07-06T14:27:03Z
dc.date.issued2018
dc.descriptionThis study had the support of the Fundação para a Ciência e a Tecnologia (FCT) Strategic Project UID/MAR/04292/2013 granted to MARE. Joana Silva, Celso Alves and Susete Pinteus were financial supported by a from FCTFundação para a Ciência e Tecnologia grants SFRH/BD/103255/2014, SFRH/ BD/977641/2013 and SFRH/BD/96203/2013, respectively.pt_PT
dc.description.abstractBackground: Parkinson’s disease (PD) is a progressive neurodegenerative disorder of the central nervous system. Although the causes of PD pathogenesis remain incomplete, some evidences has suggested that oxidative stress is an important mediator in its pathogenesis. The aim of this study was to evaluate the protective effects of seaweeds with high antioxidant activity on 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in the human neuroblastoma cell line SH-SY5Y, as well as the associated intracellular signaling pathways. Methods: Cell viability studies were assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium (MTT) bromide assay and the intracellular signaling pathways analyzed were: hydrogen peroxide (H2O2) production, changes in the mitochondrial membrane potential and Caspase-3 activity. Results: Exposure of SH-SY5Y cells to 6-OHDA (10–1000 μM) reduced cell’s viability in a concentration and timedependent manner. The data suggest that the cell death induced by 6-OHDA was mediated by an increase of H2O2 production, the depolarization of mitochondrial membrane potential and the increase of Caspase-3 activity. Extracts from S. polyshides, P. pavonica, S. muticum, C. tomentosum and U. compressa revealed to efficiently protect cell’s viability in the presence of 6-OHDA (100 μM; 24 h). These effects appear to be associated with the reduction of H2O2 cell’s production, the protection of mitochondrial membrane’s potential and the reduction of Caspase-3 activity. Conclusions: These results suggest that seaweeds can be a promising source of new compounds with neuroprotective potential.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, J., Alves, C., Pinteus, S., Mendes, S., & Pedrosa, R. (2018). Neuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma model. BMC complementary and alternative medicine, 18(1), 58. https://doi.org/10.1186/s12906-018-2103-2pt_PT
dc.identifier.doi10.1186/s12906-018-2103-2pt_PT
dc.identifier.issn1472-6882
dc.identifier.urihttp://hdl.handle.net/10400.8/4991
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBMCpt_PT
dc.relationNeuroprotective effects of molecules isolated from Bifurcaria bifurcata and Codium tomentosum in an in vitro and in vivo Parkinson Disease model.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectParkinson’s diseasept_PT
dc.subjectSubstantia nigrapt_PT
dc.subjectMitochondrial membrane potentialpt_PT
dc.subjectCaspase – 3 activitypt_PT
dc.subjectOxidative stresspt_PT
dc.subjectApoptosispt_PT
dc.subjectMarine natural bioactive compoundspt_PT
dc.titleNeuroprotective effects of seaweeds against 6-hydroxidopamine-induced cell death on an in vitro human neuroblastoma modelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNeuroprotective effects of molecules isolated from Bifurcaria bifurcata and Codium tomentosum in an in vitro and in vivo Parkinson Disease model.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F04292%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F103255%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F96203%2F2013/PT
oaire.citation.titleBMC Complementary and Alternative Medicinept_PT
oaire.citation.volume18pt_PT
oaire.fundingStream5876
oaire.fundingStreamPOR_CENTRO
oaire.fundingStreamSFRH
person.familyNameSilva
person.familyNameAlves
person.familyNamePinteus
person.familyNameMENDES
person.familyNamePedrosa
person.givenNameJoana
person.givenNameCelso
person.givenNameSusete
person.givenNameSUSANA
person.givenNameRui
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person.identifier.ciencia-id3817-33DE-919E
person.identifier.orcid0000-0003-1224-1699
person.identifier.orcid0000-0003-1581-2127
person.identifier.orcid0000-0002-3290-3111
person.identifier.orcid0000-0001-9681-3169
person.identifier.orcid0000-0003-0970-0575
person.identifier.ridB-3366-2017
person.identifier.ridB-4815-2015
person.identifier.scopus-author-id56370819800
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person.identifier.scopus-author-id55655328300
person.identifier.scopus-author-id33568138000
person.identifier.scopus-author-id7005010300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
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