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The potential of cholinesterases as tools for biomonitoring studies with sharks: Biochemical characterization in brain and muscle tissues of Prionace glauca

2015Journal article Restricted access
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dc.contributor.authorAlves, Luís M.
dc.contributor.authorLemos, Marco F.L.
dc.contributor.authorCorreia, João P. S.
dc.contributor.authorCosta, Nuno A.R. da
dc.contributor.authorNovais, Sara C.
dc.date.accessioned2022-09-12T13:03:00Z
dc.date.available2022-09-12T13:03:00Z
dc.date.issued2015
dc.description.abstractCholinesterases (ChE) are a family of enzymes that play an essential role in neuronal and motor functions. Because of the susceptibility of these enzymes to anticholinergic agents and to other contaminants, their activity is frequently used as biomarker in pollution monitoring studies. The three known types of ChE in fish are acetilcholinesterase (AChE), butyrylcholinesterase (BChE) and propionylcholinesterase (PChE). The presence of these enzymes in each tissue differs between species, and thus their usage as biomarkers requires previous enzyme characterization. Sharks, mostly acting as apex predators, help maintain the balance of fish populations performing a key role in the ecosystem. Blue sharks (Prionace glauca) are one of the most abundant and heavily fished sharks in the world, thus being good candidate organisms for ecotoxicology and biomonitoring studies. The present study aimed to characterize the ChE present in the brain and muscle of the blue shark using different substrates and selective inhibitors, and to assess the in vitro sensitivity of these sharks' ChE to chlorpyrifos-oxon, a metabolite of a commonly used organophosphorous pesticide, recognized as amodel anticholinesterase contaminant. The results suggest that the brain of P. glauca seems to contain atypical ChEs, displayingmixed properties of AChE and BChE, and that the muscle tissue seems to contain mainly AChE. In vitro exposures to chloropyrifosoxon inhibited blue shark's ChE in both tissues, the brain being the most sensitive tissue and therefore the most suitable for detection of exposure to low concentrations of anticholinergic compounds in the environment. This study indicates that ChE activity in blue sharks has the potential to be used as a sensitive and reliable biomarker in marine biomonitoring programs.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLuís M. Alves, Marco F.L. Lemos, João P.S. Correia, Nuno A.R. da Costa, Sara C. Novais, The potential of cholinesterases as tools for biomonitoring studies with sharks: Biochemical characterization in brain and muscle tissues of Prionace glauca, Journal of Experimental Marine Biology and Ecology, Volume 465, 2015, Pages 49-55, ISSN 0022-0981, https://doi.org/10.1016/j.jembe.2015.01.006pt_PT
dc.identifier.doi10.1016/j.jembe.2015.01.006pt_PT
dc.identifier.issn0022-0981
dc.identifier.issn1879-1697
dc.identifier.urihttp://hdl.handle.net/10400.8/7645
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationENVIRONOME - INTEGRATING OMICS IN ECOTOXICOLOGY: TOOLS FOR ENVIRONMENTAL RISK ASSESSMENT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0022098115000076pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBiomarkerpt_PT
dc.subjectBlue sharkpt_PT
dc.subjectChlorpyrifos-oxonpt_PT
dc.subjectPollutionpt_PT
dc.titleThe potential of cholinesterases as tools for biomonitoring studies with sharks: Biochemical characterization in brain and muscle tissues of Prionace glaucapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleENVIRONOME - INTEGRATING OMICS IN ECOTOXICOLOGY: TOOLS FOR ENVIRONMENTAL RISK ASSESSMENT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F94500%2F2013/PT
oaire.citation.endPage55pt_PT
oaire.citation.startPage49pt_PT
oaire.citation.titleJournal of Experimental Marine Biology and Ecologypt_PT
oaire.citation.volume465pt_PT
oaire.fundingStreamFARH
person.familyNameAlves
person.familyNameLemos
person.familyNameCorreia
person.familyNameNovais
person.givenNameLuís
person.givenNameMarco
person.givenNameJoão
person.givenNameSara
person.identifier996337
person.identifier414226
person.identifier.ciencia-idB615-BAFB-B8ED
person.identifier.ciencia-id971F-ACCA-C0D1
person.identifier.ciencia-id281D-9F46-4EAE
person.identifier.ciencia-id2216-A2E0-A118
person.identifier.orcid0000-0001-5106-7430
person.identifier.orcid0000-0001-9887-1864
person.identifier.orcid0000-0002-3613-5420
person.identifier.orcid0000-0003-1306-3396
person.identifier.ridF-7951-2011
person.identifier.ridA-9032-2012
person.identifier.scopus-author-id7006042884
person.identifier.scopus-author-id7202364150
person.identifier.scopus-author-id23025463300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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