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Biomimetic composite coating on rapid prototyped scaffolds for bone tissue engineering

2011-02Journal article Open access
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dc.contributor.authorArafat, M. Tarik
dc.contributor.authorLam, Christopher X.F.
dc.contributor.authorEkaputra, Andrew K.
dc.contributor.authorWong, Siew Yee
dc.contributor.authorLi, Xu
dc.contributor.authorGibson, Ian
dc.date.accessioned2026-01-12T18:49:31Z
dc.date.available2026-01-12T18:49:31Z
dc.date.issued2011-02
dc.description.abstractThe objective of this present study was to improve the functional performance of rapid prototyped scaffolds for bone tissue engineering through biomimetic composite coating. Rapid prototyped poly(ε-caprolactone)/tri-calcium phosphate (PCL/TCP) scaffolds were fabricated using the screw extrusion system (SES). The fabricated PCL/TCP scaffolds were coated with a carbonated hydroxyapatite (CHA)-gelatin composite via biomimetic co-precipitation. The structure of the prepared CHA-gelatin composite coating was studied by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. Compressive mechanical testing revealed that the coating process did not have any detrimental effect on the mechanical properties of the scaffolds. The cell-scaffold interaction was studied by culturing porcine bone marrow stromal cells (BMSCs) on the scaffolds and assessing the proliferation and bone-related gene and protein expression capabilities of the cells. Confocal laser microscopy and SEM images of the cell-scaffold constructs showed a uniformly distributed cell sheet and accumulation of extracellular matrix in the interior of CHA-gelatin composite-coated PCL/TCP scaffolds. The proliferation rate of BMSCs on CHA-gelatin composite-coated PCL/TCP scaffolds was about 2.3 and 1.7 times higher than that on PCL/TCP scaffolds and CHA-coated PCL/TCP scaffolds, respectively, by day 10. Furthermore, reverse transcription polymerase chain reaction and Western blot analysis revealed that CHA-gelatin composite-coated PCL/TCP scaffolds stimulate osteogenic differentiation of BMSCs the most, compared with PCL/TCP scaffolds and CHA-coated PCL/TCP scaffolds. These results demonstrate that CHA-gelatin composite-coated rapid prototyped PCL/TCP scaffolds are promising for bone tissue engineering.eng
dc.description.sponsorshipFinancial support for this research from the A*STAR Program under Grant No. R 397 000 038 305 is acknowledged.
dc.identifier.doi10.1016/j.actbio.2010.09.010
dc.identifier.issn1742-7061
dc.identifier.urihttp://hdl.handle.net/10400.8/15309
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier BV
dc.relation.hasversionhttps://pubmed.ncbi.nlm.nih.gov/20849985/
dc.relation.ispartofActa Biomaterialia
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleBiomimetic composite coating on rapid prototyped scaffolds for bone tissue engineeringeng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage820
oaire.citation.issue2
oaire.citation.startPage809
oaire.citation.titleActa Biomaterialia
oaire.citation.volume7
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameGibson
person.givenNameIan
person.identifier.orcid0000-0002-4149-9122
relation.isAuthorOfPublication86fbb8a7-7598-4d8c-b94f-ce5456e840e3
relation.isAuthorOfPublication.latestForDiscovery86fbb8a7-7598-4d8c-b94f-ce5456e840e3

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