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Continuous production of highly tuned silk/calcium-based composites: exploring new pathways for skin regeneration

2022Journal article Open access
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dc.contributor.authorVeiga, Anabela
dc.contributor.authorMagalhaes, Rui
dc.contributor.authorDuarte, Marta M.
dc.contributor.authorDias, Juliana R.
dc.contributor.authorAlves, Nuno M.
dc.contributor.authorCosta-Pinto, Ana Rita
dc.contributor.authorCastro, Filipa
dc.contributor.authorRocha, Fernando
dc.contributor.authorOliveira, Ana L.
dc.date.accessioned2023-02-08T11:35:15Z
dc.date.available2023-02-08T11:35:15Z
dc.date.issued2022
dc.date.updated2022-11-23T15:35:00Z
dc.description.abstractCalcium plays an important role in barrier function repair and skin homeostasis. In particular, calcium phosphates (CaPs) are well established materials for biomedical engineering due to their biocompatibility. To generate biomaterials with a more complete set of biological properties, previously discarded silk sericin (SS) has been recovered and used as a template to grow CaPs. Crucial characteristics for skin applications, such as antibacterial activity, can be further enhanced by doping CaPs with cerium (Ce) ions. The effectiveness of cell attachment and growth on the materials highly depends on their morphology, particle size distribution, and chemical composition. These characteristics can be tailored through the application of oscillatory flow technology, which provides precise mixing control of the reaction medium. Thus, in the present work, CaP/SS and CaP/SS/Ce particles were fabricated for the first time using a modular oscillatory flow plate reactor (MOFPR) in a continuous mode. Furthermore, the biological behavior of both these composites and of previously produced pure CaPs was assessed using human dermal fibroblasts (HDFs). It was demonstrated that both CaP based with plate-shaped nanoparticles and CaP-SS-based composites significantly improved cell viability and proliferation over time. The results obtained represent a first step towards the reinvention of CaPs for skin engineering.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVeiga, A., Magalhães, R., Duarte, M. M., Dias, J. R., Alves, N. M., Costa-Pinto, A. R., Castro, F., Rocha, F., & Oliveira, A. L. (2022). Continuous production of highly tuned silk/calcium-based composites: exploring new pathways for skin regeneration. Molecules, 27(7), 2249. https://doi.org/10.3390/molecules27072249pt_PT
dc.identifier.doihttps://doi.org/10.3390/molecules27072249pt_PT
dc.identifier.slugcv-prod-2728343
dc.identifier.urihttp://hdl.handle.net/10400.8/8108
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationLA/P/0045/2020 (ALiCE)pt_PT
dc.relationCentre of Biotechnology and Fine Chemistry
dc.relationUIDP/00511/2020 (LEPABE)pt_PT
dc.relationLaboratory for Process Engineering, Environment, Biotechnology and Energy
dc.relationCentre for Rapid and Sustainable Product Development
dc.relationCentre for Rapid and Sustainable Product Development
dc.relationPOCI-01-0145-FEDER-31146pt_PT
dc.relationNovo: Designing Multilevel Solutions for Skin Tissue Engineering: from Particles to 3D Functional Constructs. Inicial: Biofabrication of a 3D silk-sericin construct as a new strategy for Engineering a Skin Tissue Model
dc.relation.publisherversionhttps://www.mdpi.com/1420-3049/27/7/2249pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCalcium phosphate-based materials (CaP)pt_PT
dc.subjectCerium (Ce)pt_PT
dc.subjectHuman dermal fibroblasts (HDFs)pt_PT
dc.subjectModular oscillatory flow plate reactor (MOPR)pt_PT
dc.subjectSilk sericin (SS)pt_PT
dc.subjectSkin regenerationpt_PT
dc.titleContinuous production of highly tuned silk/calcium-based composites: exploring new pathways for skin regenerationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biotechnology and Fine Chemistry
oaire.awardTitleLaboratory for Process Engineering, Environment, Biotechnology and Energy
oaire.awardTitleCentre for Rapid and Sustainable Product Development
oaire.awardTitleCentre for Rapid and Sustainable Product Development
oaire.awardTitleNovo: Designing Multilevel Solutions for Skin Tissue Engineering: from Particles to 3D Functional Constructs. Inicial: Biofabrication of a 3D silk-sericin construct as a new strategy for Engineering a Skin Tissue Model
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50016%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00511%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04044%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04044%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/2020.08683.BD/PT
oaire.citation.endPage22pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume27pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_NORTE
person.familyNameDias
person.familyNameAlves
person.givenNameJuliana
person.givenNameNuno
person.identifier633669
person.identifier452149
person.identifier.ciencia-id0612-D971-F00D
person.identifier.ciencia-id311E-1559-8F6C
person.identifier.orcid0000-0002-7079-1069
person.identifier.orcid0000-0002-5016-0868
person.identifier.ridA-6086-2014
person.identifier.ridN-4073-2013
person.identifier.scopus-author-id55749822200
person.identifier.scopus-author-id7006403383
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid0612-D971-F00D | Juliana Rosa Dias
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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