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Cholinesterase activity in the caddisfly Sericostoma vittatum: Biochemical enzyme characterization and in vitro effects of insecticides and psychiatric drugs

2014-04-15Artigo científico Acesso restrito
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datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.fosCiências Naturais::Ciências da Terra e do Ambiente
datacite.subject.sdg14:Proteger a Vida Marinha
datacite.subject.sdg06:Água Potável e Saneamento
dc.contributor.authorPestana, João L. T.
dc.contributor.authorNovais, Sara C.
dc.contributor.authorLemos, Marco
dc.contributor.authorSoares, Amadeu M. V. M.
dc.date.accessioned2026-05-18T10:39:29Z
dc.date.available2026-05-18T10:39:29Z
dc.date.issued2014-04-15
dc.description.abstractSericostoma vittatum is a caddisfly species, endemic to the Iberian Peninsula, proposed as a biomonitor species for lotic ecosystems. Since inhibition of cholinesterases[U+05F3] (ChE) activity has been used to evaluate the exposure of macroinvertebrates to organophosphates and carbamate pesticides, this work intended to characterize the ChE present in this species so their activity can be used as a potential biomarker of exposure. Biochemical and pharmacological properties of ChE were characterized in this caddisfly species using different substrates (acetylthiocholine iodide, propionylthiocholine iodide, and butyrylthiocholine iodide) and selective inhibitors (eserine sulfate, BW284c51, and iso-OMPA). Also, the in vitro effects of two insecticides (carbaryl and chlorantraniliprole) and two psychiatric drugs (fluoxetine and carbamazepine) on ChE activity were investigated. The results suggest that S. vittatum possess mainly AChE able to hydrolyze both substrates acetylthiocholine and propionylthiocholine since: (1) it hydrolyzes the substrate acetylthiocholine and propionylcholine at similar rates and butyrylthiocholine at a much lower rate; (2) it is highly sensitive to eserine sulfate and BW284c51, but not to iso-OMPA; and (3) its activity is inhibited by excess of substrate, a characteristic of typical AChE. in vitro inhibitions were observed only for carbaryl exposure while exposure to chlorantraniliprole and to relevant environmental concentrations of psychiatric drugs did not cause any significant effect on AChE activity. This study suggests that AChE activity in caddisflies can indeed be used to discriminate the effects of specific insecticides in monitoring programs. The use of non-target species such as caddisflies in ecotoxicological research in lotic ecosystems is also discussed.eng
dc.description.sponsorshipFinancial support for this work was provided by “IDEAL – Insecticides, DEtritivores and ALiens: Combined effects of invasive species and insecticides along detritus based stream food webs”, with the reference code PTDC/AAC-AMB/119433/2010 supported by European Funds through COMPETE and by National Funds through the Portuguese Science Foundation (FCT) within project PEst-C/MAR/LA0017/2013. FCT also financed a post-doctoral research grant to João Pestana (SFRH/BPD/45342/2008).
dc.identifier.citationPestana, J. L. T., Novais, S. C., Lemos, M. F. L., & Soares, A. M. V. M. (2014). Cholinesterase activity in the caddisfly Sericostoma vittatum: Biochemical enzyme characterization and in vitro effects of insecticides and psychiatric drugs. Ecotoxicology and Environmental Safety, 104, 263-268. https://doi.org/10.1016/j.ecoenv.2014.03.018
dc.identifier.doi10.1016/j.ecoenv.2014.03.012
dc.identifier.issn0147-6513
dc.identifier.urihttp://hdl.handle.net/10400.8/16290
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationIDEAL - Insecticides, DEtritivores and ALiens: Combined effects of invasive species and insecticides along detritus based stream food webs
dc.relationStrategic Project - LA 17 - 2013-2014
dc.relationDIRECT AND INDIRECT EFFECTS OF CONTAMINANTS ON DETRITUS PROCESSING WITHIN FRESHWATER FOOD WEBS
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S0147651314001171
dc.relation.ispartofEcotoxicology and Environmental Safety
dc.rights.uriN/A
dc.subjectCarbaryl
dc.subjectChlorantraniliprole
dc.subjectCholinesterase characterization
dc.subjectPsychiatric pharmaceuticals
dc.subjectSericostoma vittatum
dc.titleCholinesterase activity in the caddisfly Sericostoma vittatum: Biochemical enzyme characterization and in vitro effects of insecticides and psychiatric drugseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPTDC/AAC-AMB/119433/2010
oaire.awardNumberPEst-C/MAR/LA0017/2013
oaire.awardNumberSFRH/BPD/45342/2008
oaire.awardTitleIDEAL - Insecticides, DEtritivores and ALiens: Combined effects of invasive species and insecticides along detritus based stream food webs
oaire.awardTitleStrategic Project - LA 17 - 2013-2014
oaire.awardTitleDIRECT AND INDIRECT EFFECTS OF CONTAMINANTS ON DETRITUS PROCESSING WITHIN FRESHWATER FOOD WEBS
oaire.awardURIhttp://hdl.handle.net/10400.8/16263
oaire.awardURIhttp://hdl.handle.net/10400.8/16264
oaire.awardURIhttp://hdl.handle.net/10400.8/15576
oaire.citation.endPage268
oaire.citation.issue1
oaire.citation.startPage263
oaire.citation.titleEcotoxicology and Environmental Safety
oaire.citation.volume104
oaire.fundingStreamConcurso de Projectos de Investigação Científica e Desenvolvimento Tecnológico em todos os Domínios Científicos - 2010
oaire.fundingStreamConcurso para Financiamento de Projectos de IC&DT Estratégicos e de Interesse Público promovidos por Laboratórios Associados e Unidades de I&D - 2013 - COMPETE
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameNovais
person.familyNameLemos
person.givenNameSara
person.givenNameMarco
person.identifier996337
person.identifier.ciencia-id2216-A2E0-A118
person.identifier.ciencia-id971F-ACCA-C0D1
person.identifier.orcid0000-0003-1306-3396
person.identifier.orcid0000-0001-9887-1864
person.identifier.ridA-9032-2012
person.identifier.ridF-7951-2011
person.identifier.scopus-author-id23025463300
person.identifier.scopus-author-id7006042884
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relation.isAuthorOfPublicationf21e5540-df76-43e9-ad64-93edd70da1f1
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