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Cell-instructive pectin hydrogels crosslinked via thiol-norbornene photo-click chemistry for skin tissue engineering

2017-11-08Journal article Open access
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dc.contributor.authorPereira, Rúben
dc.contributor.authorBarrias, Cristina C.
dc.contributor.authorBártolo, Paulo J.
dc.contributor.authorGranja, Pedro L.
dc.date.accessioned2023-05-26T14:49:13Z
dc.date.available2023-05-26T14:49:13Z
dc.date.issued2017-11-08
dc.description.abstractCell-instructive hydrogels are attractive for skin repair and regeneration, serving as interactive matrices to promote cell adhesion, cell-driven remodeling and de novo deposition of extracellular matrix compo nents. This paper describes the synthesis and photocrosslinking of cell-instructive pectin hydrogels using cell-degradable peptide crosslinkers and integrin-specific adhesive ligands. Protease-degradable hydro gels obtained by photoinitiated thiol-norbornene click chemistry are rapidly formed in the presence of dermal fibroblasts, exhibit tunable properties and are capable of modulating the behavior of embedded cells, including the cell spreading, hydrogel contraction and secretion of matrix metalloproteases. Keratinocytes seeded on top of fibroblast-loaded hydrogels are able to adhere and form a compact and dense layer of epidermis, mimicking the architecture of the native skin. Thiol-ene photocrosslinkable pec tin hydrogels support the in vitro formation of full-thickness skin and are thus a highly promising plat form for skin tissue engineering applications, including wound healing and in vitro testing modpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationúben F. Pereira, Cristina C. Barrias, Paulo J. Bártolo, Pedro L. Granja, Cell-instructive pectin hydrogels crosslinked via thiol-norbornene photo-click chemistry for skin tissue engineering, Acta Biomaterialia, Volume 66, 2018, Pages 282-293, ISSN 1742-7061, https://doi.org/10.1016/j.actbio.2017.11.01pt_PT
dc.identifier.doi10.1016/j.actbio.2017.11.01pt_PT
dc.identifier.issn1878-7568
dc.identifier.urihttp://hdl.handle.net/10400.8/8525
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1742706117307006pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectThiol-norbornenept_PT
dc.subjectPhotocrosslinkingpt_PT
dc.subjectPectinpt_PT
dc.subjectHydrogelpt_PT
dc.subjectDermal fibroblastspt_PT
dc.subjectSkinpt_PT
dc.titleCell-instructive pectin hydrogels crosslinked via thiol-norbornene photo-click chemistry for skin tissue engineeringpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage293pt_PT
oaire.citation.startPage282pt_PT
oaire.citation.titleActa Biomaterialiapt_PT
oaire.citation.volume66pt_PT
person.familyNameBrás Pereira
person.givenNameRúben Filipe
person.identifier1337969
person.identifier.ciencia-id9417-153E-4473
person.identifier.orcid0000-0002-7255-3400
person.identifier.ridP-8503-2018
person.identifier.scopus-author-id55421048100
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationeda35774-59b3-4801-aa88-9c20f4c31a78
relation.isAuthorOfPublication.latestForDiscoveryeda35774-59b3-4801-aa88-9c20f4c31a78

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