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Effect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276

2020Journal article Restricted access
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dc.contributor.authorPaulo, M.C.
dc.contributor.authorCoutinho, J.
dc.contributor.authorSaraiva, J.A.
dc.contributor.authorPinto, C.A.
dc.contributor.authorAmaral, R.
dc.contributor.authorMelo, M.M.R.
dc.contributor.authorPinheiro, J.
dc.contributor.authorLemos, M.F.L.
dc.contributor.authorCardoso, C.
dc.contributor.authorSapatinha, M.
dc.contributor.authorBandarra, N.M.
dc.date.accessioned2021-08-20T14:16:58Z
dc.date.available2021-08-20T14:16:58Z
dc.date.issued2020
dc.description.abstractThere has been a growing trend worldwide in the use of squalene and docosahexaenoic acid (DHA), due in part to their recognized health benefits and their range of applications. However, the growth of the industry exploiting these resources has been extremely limited by the scarcity and limitations of raw material, leading to the need to develop more sustainable sources of production. Aurantiochytrium sp. PRA-276, with the ability to produce high concentrations of DHA and squalene, is a promising candidate for the commercial production of these biomolecules. The aim of this study was to determine the cumulative effect of airflow levels and stirring speed of Aurantiochytrium sp. PRA-276 biomass during DHA and squalene production. Different airflow levels (3, 6 and 9 L min-1) and two stirring speeds (150 and 300 rpm) were investigated. The highest biomass production was achieved after 96 hours with 300 rpm stirring speeds and airflow level of 9 L min-1. The maximum total lipids content reached 61.4% of the dried biomass, and the optimal parameters for DHA and squalene production were stirring speed 150 rpm and airflow level 3 L min-1. The maximum yield of DHA production was 4.3 g L-1 at 96 h of fermentation. The highest yield of squalene production was 762.5 mg L-1 at 48 hours of growth. The results show that Aurantiochytrium sp. PRA-276 is a promising resource for microbial DHA and squalene production due to the high yield of DHA and squalene that it generates. Moreover, the capacity for large-scale fermentation of this organism could be useful for the synthesis of biomass related products with high added value.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPaulo, M.C., Coutinho, J., Saraiva, J.A., Pinto, C.A., Amaral, R., Melo, M.M.R., Pinheiro, J., Lemos, M.F.L., Cardoso, C., Sapatinha, M., & Bandarra, N.M. (2020). Effect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276. Journal of Nutritional Biology, 6(1), 420-429. https://doi.org/10.18314/jnb.v6i1.2060pt_PT
dc.identifier.doi10.18314/jnb.v6i1.2060pt_PT
dc.identifier.issn2469-4142
dc.identifier.urihttp://hdl.handle.net/10400.8/6127
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherGratispt_PT
dc.relationTHE FORMATION OF THE DUTCH AND PORTUGUESE ATLANTIC EMPIRES: THE ROLE OF EUROPEANS AND AFRICANS IN THE WEST COAST OF AFRICA, 1600-1800
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAurantiochytrium sppt_PT
dc.subjectSqualenept_PT
dc.subjectDocosahexaenoic acid (DHA)pt_PT
dc.subjectLipidspt_PT
dc.subjectHeterotrophic marine algaept_PT
dc.titleEffect of airflow conditions on growth and simultaneously DHA and squalene production by Aurantiochytrium sp. PRA-276pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTHE FORMATION OF THE DUTCH AND PORTUGUESE ATLANTIC EMPIRES: THE ROLE OF EUROPEANS AND AFRICANS IN THE WEST COAST OF AFRICA, 1600-1800
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F17680%2F2004/PT
oaire.citation.endPage429pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage420pt_PT
oaire.citation.titleJournal of Nutritional Biologypt_PT
oaire.citation.volume6pt_PT
person.familyNamePinheiro
person.familyNameLemos
person.familyNameBandarra
person.givenNameJoaquina
person.givenNameMarco
person.givenNameNarcisa
person.identifier556456
person.identifier996337
person.identifier.ciencia-idB113-2D80-406B
person.identifier.ciencia-id6316-47CA-37ED
person.identifier.orcid0000-0001-9319-2651
person.identifier.orcid0000-0001-9887-1864
person.identifier.orcid0000-0002-7563-9226
person.identifier.ridF-7951-2011
person.identifier.ridC-4780-2013
person.identifier.scopus-author-id25228814000
person.identifier.scopus-author-id7006042884
person.identifier.scopus-author-id6602160562
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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