Repository logo
 
Publication

Camelina oil as a source of polyunsaturated fatty acids for the production of human milk fat substitutes catalyzed by a heterologous Rhizopus oryzae lipase

2015-07-07Journal article Restricted access
Simple item page
datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg13:Ação Climática
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authorFaustino, Ana Rita
dc.contributor.authorOsório, Natália M.
dc.contributor.authorTecelão, Carla
dc.contributor.authorCanet, Albert
dc.contributor.authorValero, Francisco
dc.contributor.authorFerreira‐Dias, Suzana
dc.date.accessioned2025-06-12T16:42:13Z
dc.date.available2025-06-12T16:42:13Z
dc.date.issued2015-07-07
dc.description.abstractThis study aims to produce human milk fat substitutes (HMFS) rich in polyunsaturated fatty acids (PUFA),mainly the essential omega-3 linolenic acid, by acidolysis reaction between tripalmitin (PPP) and free fattyacids (FFA) from camelina oil, in stirred batch reactor and solvent-free media. The non-commercialheterologous Rhizopus oryzae lipase (rROL), immobilized on Lewatit VP OC 1600 or on Relizyme OD403/S, and the immobilized commercial lipase from Rhizomucor miehei (Lipozyme RM IM) were tested asbiocatalysts. Both lipases are sn-1,3 selective. After 24 h reaction at 60°C, using biocatalyst loads of 5 wt%(in relation to tripalmitin), 48.9, 43.6, and 18.3 mol% of fatty acid incorporation in triacylglycerols (TAG)were obtained with Lipozyme RM IM and rROL immobilized on Lewatit or on Relizyme, respectively.rROL immobilized on Lewatit was selected as biocatalyst for the acidolysis, as alternative to the commercialimmobilized lipases. With this biocatalyst, the molar incorporation increased with its initial water activity(0.55–0.95). Modeling acidolysis catalyzed by rROL immobilized on Lewatit was performed by responsesurface methodology, as a function of temperature (58–72°C) and molar ratio FFA:PPP (1.2:1–6.8:1). Thehighest PPP consumption was achieved at 60°C with a molar ratio of 2:1. The yield of HMFS (new TAG)increased from 42.6 wt% (5% biocatalyst load) to 52% with 8% load, after 24 h acidolysis.Practical applications: Camelina oil showed to be a good source of PUFA, mainly essential fatty acids,to incorporate in HMFS. After 24 h acidolysis under optimized conditions, catalyzed by the non-commercial sn-1,3 regioselective rROL immobilized on Lewatit VP OC 1600, the TAG fraction contains67.7 mol% of palmitic acid at position 2. These structured lipids rich in PUFA can be used in blends with1,3-dioleoyl-2-palmitoyl-glycerol (OPO) in order to mimic the human milk fat. The performance of thisbiocatalyst was comparable to that observed with Lipozyme RM IM. The replacement of high-costcommercial immobilized lipases by rROL immobilized on Lewatit may reduce the biocatalyst cost. Inaddition, since the best molar ratio FFA:PPP for rROL is 2:1, i.e., the stoichiometric value for theacidolysis catalyzed by sn-1,3 regioselective lipases, the use of this biocatalyst will reduce downstreamcosts related with unconverted FFA recovery.por
dc.description.sponsorshipThis study was supported by: (i) the Strategic Project PEst-OE/AGR/UI0245/2014 of CEER, Biosystems Engineering, a research unit supported by the national funding of Fundação para a Ciência e a Tecnologia, Portugal; (ii) the Integrated Action Portugal-Spain E-29/11; and (iii) the project CTQ2013-42391-R of the Spanish Ministry of Economy and Competitivity. The Spanish research group is member of the Reference Network in Biotechnology (XRB) (Generalitat de Catalunya) and the 2014-SGR-452.
dc.identifier.citationFaustino, A.R., Osório, N.M., Tecelão, C., Canet, A., Valero, F. and Ferreira-Dias, S. (2016), Camelina oil as a source of polyunsaturated fatty acids for the production of human milk fat substitutes catalyzed by a heterologous Rhizopus oryzae lipase. Eur. J. Lipid Sci. Technol., 118: 532-544. https://doi.org/10.1002/ejlt.201500003
dc.identifier.doi10.1002/ejlt.201500003
dc.identifier.issn1438-7697
dc.identifier.issn1438-9312
dc.identifier.urihttp://hdl.handle.net/10400.8/13219
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relation.hasversionhttps://onlinelibrary.wiley.com/doi/10.1002/ejlt.201500003
dc.relation.ispartofEuropean Journal of Lipid Science and Technology
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAcidolysis
dc.subjectCamelina oil
dc.subjectHuman milk fat substitutes
dc.subjectPolyunsaturated fatty acids
dc.subjectRhizopus oryzaelipase
dc.subjectStructured lipids
dc.titleCamelina oil as a source of polyunsaturated fatty acids for the production of human milk fat substitutes catalyzed by a heterologous Rhizopus oryzae lipaseeng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage544
oaire.citation.issue4
oaire.citation.startPage532
oaire.citation.titleEuropean Journal of Lipid Science and Technology
oaire.citation.volume118
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameTecelão
person.givenNameCarla
person.identifier.ciencia-id9B1D-4AC0-1B03
person.identifier.orcid0000-0003-2423-0495
person.identifier.scopus-author-id7801371939
relation.isAuthorOfPublication0d890808-4e69-4e6c-a631-802f1a7dce1f
relation.isAuthorOfPublication.latestForDiscovery0d890808-4e69-4e6c-a631-802f1a7dce1f

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Euro J Lipid Sci Tech - 2015 - Faustino - Camelina oil as a source of polyunsaturated fatty acids for the production of.pdf
Size:
929.59 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.32 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

MARE - Artigos com peer review
ESTM - Artigos em revistas internacionais