Publication
Induction of multixenobiotic defense mechanisms in resistant Daphnia magna clones as a general cellular response to stress
| datacite.subject.sdg | 12:Produção e Consumo Sustentáveis | |
| datacite.subject.sdg | 13:Ação Climática | |
| datacite.subject.sdg | 14:Proteger a Vida Marinha | |
| dc.contributor.author | Jordão, Rita | |
| dc.contributor.author | Campos, Bruno | |
| dc.contributor.author | Lemos, Marco | |
| dc.contributor.author | Soares, Amadeu M.V.M. | |
| dc.contributor.author | Tauler, Romà | |
| dc.contributor.author | Barata, Carlos | |
| dc.date.accessioned | 2025-06-12T13:39:05Z | |
| dc.date.available | 2025-06-12T13:39:05Z | |
| dc.date.issued | 2016-06 | |
| dc.description.abstract | Multixenobiotic resistance mechanisms (MXR) were recently identified in Daphnia magna. Previous results characterized gene transcripts of genes encoding and efflux activities of four putative ABCB1 and ABCC transporters that were chemically induced but showed low specificity against model transporter substrates and inhibitors, thus preventing us from distinguishing between activities of different efflux transporter types. In this study we report on the specificity of induction of ABC transporters and of the stress protein hsp70 in clones selected to be genetically resistant to ABCB1 chemical substrates. Clones resistant to mitoxantrone, ivermectin and pentachlorophenol showed distinctive transcriptional responses of transporter protein coding genes and of putative transporter dye activities. Expression of hsp70 proteins also varied across resistant clones. Clones resistant to mitoxantrone and pentachlorophenol showed high constitutive levels of hsp70. Transcriptional levels of the abcb1 gene transporter and of putative dye transporter activity were also induced to a greater extent in the pentachlorophenol resistant clone. Observed higher dye transporter activities in individuals from clones resistant to mitoxantrone and ivermectin were unrelated with transcriptional levels of the studied four abcc and abcb1 transporter genes. These findings suggest that Abcb1 induction in D. magna may be a part of a general cellular stress response. | eng |
| dc.description.sponsorship | This work was supported by the Spanish MEC grants CTM2011-30471-C02-01 and CTM2014-51985-R, and by the Advancegrant−EU grants ERC-2012-AdG-320737. The Portuguese Foun-dation for Science and Technology (FCT) supported the doctoralfellowship of Rita Jordão (SFRH/BD/79453/2011), through the Portuguese Ministry of Education and Science and ESF funds (ProgramPOPH-QREN). We thank to anonymous referees for their construc-tive suggestions that improved the study. | |
| dc.identifier.citation | Rita Jordão, Bruno Campos, Marco F.L. Lemos, Amadeu M.V.M. Soares, Romà Tauler, Carlos Barata, Induction of multixenobiotic defense mechanisms in resistant Daphnia magna clones as a general cellular response to stress, Aquatic Toxicology, Volume 175, 2016, Pages 132-143, ISSN 0166-445X, https://doi.org/10.1016/j.aquatox.2016.03.015 | |
| dc.identifier.doi | 10.1016/j.aquatox.2016.03.015 | |
| dc.identifier.issn | 0166-445X | |
| dc.identifier.uri | http://hdl.handle.net/10400.8/13210 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Elsevier BV | |
| dc.relation.ispartof | Aquatic Toxicology | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Hsp70 | |
| dc.subject | Induction | |
| dc.subject | P-glycoprotein | |
| dc.subject | MDR | |
| dc.subject | Daphnia magna | |
| dc.title | Induction of multixenobiotic defense mechanisms in resistant Daphnia magna clones as a general cellular response to stress | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 143 | |
| oaire.citation.startPage | 132 | |
| oaire.citation.title | Aquatic Toxicology | |
| oaire.citation.volume | 175 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Lemos | |
| person.givenName | Marco | |
| person.identifier | 996337 | |
| person.identifier.orcid | 0000-0001-9887-1864 | |
| person.identifier.rid | F-7951-2011 | |
| person.identifier.scopus-author-id | 7006042884 | |
| relation.isAuthorOfPublication | f21e5540-df76-43e9-ad64-93edd70da1f1 | |
| relation.isAuthorOfPublication.latestForDiscovery | f21e5540-df76-43e9-ad64-93edd70da1f1 |