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In vitro evaluation of the antibacterial and antioxidant activities of extracts of Gracilaria gracilis with a view into its potential use as an additive in fish feed

2021Journal article Open access
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dc.contributor.authorAfonso, Clélia
dc.contributor.authorCorreia, Ana
dc.contributor.authorFreitas, Marta
dc.contributor.authorMouga, Teresa
dc.contributor.authorBaptista, Teresa
dc.date.accessioned2021-07-20T14:30:33Z
dc.date.available2021-07-20T14:30:33Z
dc.date.issued2021
dc.descriptionThis study had the support of Fundação para a Ciência e Tecnologia (FCT) through the strategic project UIDB/04292/2020 granted to MARE and the project Operational Programme MAR2020 through the project 16-02-01-FMP-84–Seaweedfeeds.
dc.description.abstractFish in aquaculture systems are subject to several stressors that inhibit the immune response and potentiate the development of disease and increased mortality. The inclusion of additives in the fish diet, namely seaweeds or their extracts, that are natural sources of bioactive compounds can be an important tool for promoting the health and well-being of these animals. The present study aims at the development of sustainable and effective methodologies for the extraction of bioactive compounds of the red seaweed Gracilaria gracilis, exploring its antibacterial and antioxidant potential and considering its potential use as an additive for functional fish feeds. The yield of the extraction methods was evaluated upon the use of sequential solid–liquid extraction techniques with ethanol and water as solvents, different extraction temperatures (room temperature: 40ºC and 70ºC), and extraction time. The results demonstrated that the adoption extraction times of 30 min. at 40ºC provided higher yields. We also evaluated the antioxidant capacity and the antibacterial properties of the obtained extracts against different strains that cause fish diseases by disk diffusion and broth microdilution methods. The antioxidant activity was determined by the DPPH reducing capacity method and quantification of total polyphenols content (TPC).With these results, we can establish extraction procedures that allow the future use of G. gracilis extracts, with antibacterial and antioxidant effects in a safe and effective way.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAfonso, C.; Correia, A.P.; Freitas, M.V.; Mouga, T.; Baptista, T. In Vitro Evaluation of the Antibacterial and Antioxidant Activities of Extracts of Gracilaria gracilis with a View into Its Potential Use as an Additive in Fish Feed. Appl. Sci. 2021, 11, 6642. https://doi.org/ 10.3390/app11146642pt_PT
dc.identifier.doi10.3390/app11146642pt_PT
dc.identifier.issn2076-3417
dc.identifier.urihttp://hdl.handle.net/10400.8/5906
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMarine and Environmental Sciences Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMarine seaweedpt_PT
dc.subjectRhodophytapt_PT
dc.subjectGracilariodpt_PT
dc.subjectAntimicrobialpt_PT
dc.subjectAntioxidantpt_PT
dc.subjectQuantification of total polyphenolspt_PT
dc.subjectDPPHpt_PT
dc.titleIn vitro evaluation of the antibacterial and antioxidant activities of extracts of Gracilaria gracilis with a view into its potential use as an additive in fish feedpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT
oaire.citation.titleApplied Sciencespt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAfonso
person.familyNameCorreia
person.familyNameFreitas
person.familyNameMouga
person.familyNameBaptista
person.givenNameClélia
person.givenNameAna Patrícia
person.givenNameMarta V.
person.givenNameTeresa
person.givenNameTeresa
person.identifier153274
person.identifier572069
person.identifier.ciencia-idC512-13C3-50DD
person.identifier.ciencia-id5F1C-3FBA-4FE5
person.identifier.ciencia-id001C-62E2-F6AD
person.identifier.ciencia-idE312-FDCC-1ED8
person.identifier.ciencia-idA61F-25C1-034A
person.identifier.orcid0000-0002-4167-5237
person.identifier.orcid0000-0001-6885-6495
person.identifier.orcid0000-0002-1164-224X
person.identifier.orcid0000-0003-4849-8840
person.identifier.orcid0000-0003-1168-7461
person.identifier.ridB-1211-2016
person.identifier.ridY-5518-2018
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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