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C2-Domain Abscisic Acid-Related Proteins Mediate the Interaction of PYR/PYL/RCAR Abscisic Acid Receptors with the Plasma Membrane and Regulate Abscisic Acid Sensitivity in Arabidopsis

2014-12-02Artigo científico Acesso aberto
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datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.sdg15:Proteger a Vida Terrestre
dc.contributor.authorRodriguez, Lesia
dc.contributor.authorGonzalez-Guzman, Miguel
dc.contributor.authorDiaz, Maira
dc.contributor.authorRodrigues, Américo
dc.contributor.authorIzquierdo-Garcia, Ana C.
dc.contributor.authorPeirats-Llobet, Marta
dc.contributor.authorFernandez, Maria A.
dc.contributor.authorAntoni, Regina
dc.contributor.authorFernandez, Daniel
dc.contributor.authorMarquez, Jose A.
dc.contributor.authorMulet, Jose M.
dc.contributor.authorAlbert, Armando
dc.contributor.authorRodriguez, Pedro L.
dc.date.accessioned2026-04-28T15:03:41Z
dc.date.available2026-04-28T15:03:41Z
dc.date.issued2014-12-02
dc.description.abstractMembrane-delimited abscisic acid (ABA) signal transduction plays a critical role in early ABA signaling, but the molecular mechanisms linking core signaling components to the plasma membrane are unclear. We show that transient calcium-dependent interactions of PYR/PYL ABA receptors with membranes are mediated through a 10-member family of C2-domain ABA-related (CAR) proteins in Arabidopsis thaliana. Specifically, we found that PYL4 interacted in an ABA independent manner with CAR1 in both the plasma membrane and nucleus of plant cells. CAR1 belongs to a plant specific gene family encoding CAR1 to CAR10 proteins, and bimolecular fluorescence complementation and coimmunoprecipitation assays showed that PYL4-CAR1 as well as other PYR/PYL-CAR pairs interacted in plant cells. The crystal structure of CAR4 was solved, which revealed that, in addition to a classical calcium-dependent lipid binding C2 domain, a specific CAR signature is likely responsible for the interaction with PYR/PYL receptors and their recruitment to phospholipid vesicles. This interaction is relevant for PYR/PYL function and ABA signaling, since different car triple mutants affected in CAR1, CAR4, CAR5, andCAR9 genes showed reduced sensitivity to ABA in seedling establishment and root growth assays. In summary, we identified PYR/PYL-interacting partners that mediate a transient Ca2+-dependent interaction with phospholipid vesicles, which affects PYR/PYL subcellular localization and positively regulates ABA signaling.eng
dc.description.sponsorshipThis work was supported by the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional, and Consejo Superior de Investigaciones Cientificas (Grants BIO2011-23446 to P.L.R and BFU2011-25384 to A.A.; fellowships to L.R., R.A., and A.C.I.-G.; BES 2009-016569; JAE-DOC contract to M.G.-G.) as well as the Senacyt Ifarhu (Panama) (fellowship to M.D.).
dc.description.sponsorshipSenacyt Ifarhu (Panamá) para a bolsa de Maira Diaz.
dc.identifier.citationRodriguez, L., Gonzalez-Guzman, M., Diaz, M., Rodrigues, A., Izquierdo-Garcia, A. C., Peirats-Llobet, M., Fernandez, M. A., Antoni, R., Fernandez, D., Marquez, J. A., Mulet, J. M., Albert, A., & Rodriguez, P. L. (2014). C2-Domain Abscisic Acid-Related Proteins Mediate the Interaction of PYR/PYL/RCAR Abscisic Acid Receptors with the Plasma Membrane and Regulate Abscisic Acid Sensitivity in Arabidopsis. The Plant Cell, 26(12), 4802–4820. https://doi.org/10.1105/tpc.114.129973
dc.identifier.doi10.1105/tpc.114.129973
dc.identifier.eissn1532-298X
dc.identifier.issn1040-4651
dc.identifier.urihttp://hdl.handle.net/10400.8/16212
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAmerican Society of Plant Biologists
dc.relation"BFU2011-25384"
dc.relation.hasversionhttps://academic.oup.com/plcell/article/26/12/4802/6102427
dc.rights.uriN/A
dc.subjectAbscisic acid
dc.subjectSignal transduction
dc.subjectProtein interaction
dc.subjectArabidopsis
dc.subjectPlasma membrane
dc.titleC2-Domain Abscisic Acid-Related Proteins Mediate the Interaction of PYR/PYL/RCAR Abscisic Acid Receptors with the Plasma Membrane and Regulate Abscisic Acid Sensitivity in Arabidopsiseng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage4820
oaire.citation.issue12
oaire.citation.startPage4802
oaire.citation.titleThe Plant Cell
oaire.citation.volume26
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameRodrigues
person.givenNameAmérico
person.identifier.orcid0000-0002-5293-5583
person.identifier.ridF-9573-2010
person.identifier.scopus-author-id15745701600
relation.isAuthorOfPublication718ad6e8-1d3a-4ce3-9870-e4ed6732c30b
relation.isAuthorOfPublication.latestForDiscovery718ad6e8-1d3a-4ce3-9870-e4ed6732c30b

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