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A new biocatalyst: Penicillin G acylase immobilized in sol‐gel micro‐particles with magnetic properties

2009-05Journal article Open access
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dc.contributor.authorBernardino, Susana M. S. A.
dc.contributor.authorFernandes, Pedro
dc.contributor.authorP. Fonseca, Luís
dc.contributor.authorBernardino, Susana
dc.date.accessioned2025-05-05T14:31:47Z
dc.date.available2025-05-05T14:31:47Z
dc.date.issued2009-05
dc.description.abstractThe present work focuses on the development and basic characterization of a new magnetic biocatalyst, namely penicillin G acylase (PGA), immobilized in sol-gel matrices with magnetic properties, ultimately aimed for application in cephalexin (CEX) synthesis. A mechanically stable carrier, based on porous xerogels silica matrixes starting from tetramethoxysilane (TMOS), was prepared leading to micro-carriers with medium sized particles of 30 μm, as determined by scanning electron microscopy. An immobilization yield of 95-100% and a recovered activity of 50-65% at 37°C, as determined by penicillin G (PG) hydrolysis (pH STAT method), were observed. These results clearly exceed those reported in a previous work on PGA immobilization in sol-gel, where only 10% of activity was recovered. The values of activity were kept constant for 6 months. Immobilized PGA (682 U/gdry weight) retained high specific activity throughout ten consecutive runs for PG hydrolysis, suggesting adequate biocatalyst stability. The CEX synthesis was performed at 14°C, using the free and immobilized PGA in aqueous medium. Phenylglycine methyl ester was used as acyl donor at 90 mM and 7-aminodeacetoxycephalosporanic acid was the limiting substrate at 30 mM. The CEX stoichiometric yield after 1-h reaction was close to 68% (23 mM CEX/h) and 65% (19 mM CEX/h), respectively.eng
dc.description.sponsorshipS.M.S.A. Bernardino and P. Fernandes acknowledge Fundação para a Ciência e a Tecnologia (Portugal) for financial support in the form of the PhD grant SFRH/BD/30632/2006 and for a contract under Programme Ciência 2007, respectively.
dc.identifier.citationBernardino, S.M.S.A., Fernandes, P. and Fonseca, L.P. (2009), A new biocatalyst: Penicillin G acylase immobilized in sol-gel micro-particles with magnetic properties. Biotechnology Journal, 4: 695-702. https://doi.org/10.1002/biot.200800287.
dc.identifier.doi10.1002/biot.200800287
dc.identifier.eissn1860-7314
dc.identifier.issn1860-6768
dc.identifier.urihttp://hdl.handle.net/10400.8/12848
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley-VCH Verlag
dc.relationSFRH/BD/30632/2006
dc.relation.hasversionhttps://analyticalsciencejournals.onlinelibrary.wiley.com/action/showCitFormats?doi=10.1002%2Fbiot.200800287&mobileUi=0
dc.relation.ispartofBiotechnology Journal
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCephalexin
dc.subjectImmobilization
dc.subjectPenicillin G acylase
dc.subjectSol-gel
dc.subjectBiocatalysis
dc.titleA new biocatalyst: Penicillin G acylase immobilized in sol‐gel micro‐particles with magnetic propertieseng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage702
oaire.citation.issue5
oaire.citation.startPage695
oaire.citation.titleBiotechnology Journal
oaire.citation.volume4
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameBernardino
person.givenNameSusana
person.identifier.ciencia-idE418-2945-9EB6
person.identifier.orcid0000-0001-9454-3281
person.identifier.scopus-author-id14824775000
relation.isAuthorOfPublication9d6614e3-975b-431b-bb0a-00179588eca2
relation.isAuthorOfPublication.latestForDiscovery9d6614e3-975b-431b-bb0a-00179588eca2

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The present work focuses on the development and basic characterization of a new magnetic biocatalyst, namely penicillin G acylase (PGA), immobilized in sol-gel matrices with magnetic properties, ultimately aimed for application in cephalexin (CEX) synthesis. A mechanically stable carrier, based on porous xerogels silica matrixes starting from tetramethoxysilane (TMOS), was prepared leading to micro-carriers with medium sized particles of 30 μm, as determined by scanning electron microscopy. An immobilization yield of 95-100% and a recovered activity of 50-65% at 37°C, as determined by penicillin G (PG) hydrolysis (pH STAT method), were observed. These results clearly exceed those reported in a previous work on PGA immobilization in sol-gel, where only 10% of activity was recovered. The values of activity were kept constant for 6 months. Immobilized PGA (682 U/gdry weight) retained high specific activity throughout ten consecutive runs for PG hydrolysis, suggesting adequate biocatalyst stability. The CEX synthesis was performed at 14°C, using the free and immobilized PGA in aqueous medium. Phenylglycine methyl ester was used as acyl donor at 90 mM and 7-aminodeacetoxycephalosporanic acid was the limiting substrate at 30 mM. The CEX stoichiometric yield after 1-h reaction was close to 68% (23 mM CEX/h) and 65% (19 mM CEX/h), respectively.
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