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Lymphocyte genotoxicity and protective effect of Calyptranthes tricona (Myrtaceae) against H 2 O 2-induced cell death in MCF-7 cells

2017Journal article Restricted access
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dc.contributor.authorKich, Débora Mara
dc.contributor.authorBitencourt, Shanna
dc.contributor.authorCaye, Bruna
dc.contributor.authorFaleiro, Dalana
dc.contributor.authorAlves, Celso
dc.contributor.authorSilva, Joana
dc.contributor.authorPinteus, Susete
dc.contributor.authorMergener, Michelle
dc.contributor.authorMajolo, Fernanda
dc.contributor.authorBoligon, Aline Augusti
dc.contributor.authorSantos, Roberto Christ Vianna
dc.contributor.authorPedrosa, Rui
dc.contributor.authorSouza, Claucia Fernanda Volken de
dc.contributor.authorGoettert, Márcia Inês
dc.date.accessioned2021-08-24T15:11:12Z
dc.date.available2021-08-24T15:11:12Z
dc.date.issued2017
dc.description.abstractCalyptranthes tricona is a species (Myrtaceae) native to South Brazil. Plants belonging to this family are folkloric used for analgesia, inflammation, and infectious diseases. However, little is known about the toxic potential of C. tricona. The present study aimed to evaluate the antioxidant activity of C. tricona ethanol and hexane leaf extracts, as well as verify their effect on human lymphocytes and MCF-7 cells. The extracts were subjected to preliminary phytochemical screening, antioxidant activity using DPPH and ORAC methods. Genotoxic and mutagenic effects in cultured human lymphocytes were assessed using the comet assay and the micronucleus assay, respectively. In addition, cell viability by MTT assay and fluorometric analysis of mitochondrial potential and caspases-9 activity were performed in order to verify the possible effects of both extracts on H2O2-induced cell death of MCF-7 cells. Our findings revealed that the phenol content and the antioxidant activity were only present in the ethanol extract. Also, the phytochemical screening presented steroids, triterpenoids, condensed tannins, and flavones as the main compounds. However, both extracts were capable of inducing concentration-dependent DNA damage in human lymphocytes. When treating MCF-7 cells with the extracts, both of them inhibited MCF-7 cell death in response to oxidative stress through a decrease of mitochondrial depolarization and caspases-9 activity. Thus, our results need to be considered in future in vitro and in vivo studies of C. tricona effects. In the meanwhile, we recommend caution in the acute/chronic use of this homemade preparation for medicinal purpose.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationKich DM, Bitencourt S, Caye B, Faleiro D, Alves C, Silva J, Pinteus S, Mergener M, Majolo F, Boligon AA, Santos RC, Pedrosa R, de Souza CF, Goettert MI. Lymphocyte genotoxicity and protective effect of Calyptranthes tricona (Myrtaceae) against H2O2-induced cell death in MCF-7 cells. Mol Cell Biochem. 2017 Jan;424(1-2):35-43. https://doi.org/10.1007/s11010-016-2840-9. Epub 2016 Oct 4. PMID: 27704465.pt_PT
dc.identifier.doi10.1007/s11010-016-2840-9pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.8/6147
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007%2Fs11010-016-2840-9pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCalyptranthes triconapt_PT
dc.subjectCytoprotectionpt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectHuman lymphocytespt_PT
dc.subjectMCF-7pt_PT
dc.titleLymphocyte genotoxicity and protective effect of Calyptranthes tricona (Myrtaceae) against H 2 O 2-induced cell death in MCF-7 cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage43pt_PT
oaire.citation.issue1-2pt_PT
oaire.citation.startPage35pt_PT
oaire.citation.titleMolecular and Cellular Biochemistrypt_PT
oaire.citation.volume424pt_PT
person.familyNameAlves
person.familyNameSilva
person.familyNamePinteus
person.familyNamePedrosa
person.givenNameCelso
person.givenNameJoana
person.givenNameSusete
person.givenNameRui
person.identifier159091
person.identifier98885
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person.identifier.orcid0000-0003-1581-2127
person.identifier.orcid0000-0003-1224-1699
person.identifier.orcid0000-0002-3290-3111
person.identifier.orcid0000-0003-0970-0575
person.identifier.ridB-3366-2017
person.identifier.ridB-4815-2015
person.identifier.scopus-author-id55654969100
person.identifier.scopus-author-id56370819800
person.identifier.scopus-author-id55655328300
person.identifier.scopus-author-id7005010300
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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