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Effect of cellulase adsorption on the surface and interfacial properties of cellulose

1999-12Journal article Restricted access
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dc.contributor.authorDourado, F.
dc.contributor.authorPala, Helena
dc.contributor.authorMota, Manuel
dc.contributor.authorGama, Francisco Miguel
dc.date.accessioned2009-08-12T15:19:10Z
dc.date.available2009-08-12T15:19:10Z
dc.date.issued1999-12
dc.description.abstractThe surface properties of several purified cellulose (Sigmacell 101, Sigmacell 20, Avicel pH 101, andWhatman CF 11) were characterised, before and after cellulase adsorption. The following techniques were used: thin-layer wicking (except for the cellulose Whatman), thermogravimetry, and differential scanning calorimetry (for all of the above celluloses). The results obtained from the calorimetric assays were consistent with those obtained from thinlayer wicking – Sigmacell 101, a more amorphous cellulose, was the least hydrophobic of the analysed celluloses, and had the highest specific heat of dehydration. The other celluloses showed less affinity for water molecules, as assessed by the two independent techniques. The adsorption of protein did not affect the amount of water adsorbed by Sigmacell 101. However, this water was more strongly adsorbed, since it had a higher specific heat of dehydration. The more crystalline celluloses adsorbed a greater amount of water, which was also more strongly bound after the treatment with cellulases. This effect was more significant for Whatman CF-11. Also, the more crystalline celluloses became slightly hydrophilic, following protein adsorption, as assessed by thin-layer wicking. However, this technique is not reliable when used with cellulase treated celluloses.pt
dc.identifier.citationDOURADO, F. e outros - Effect of cellulase adsorption on the surface and interfacial properties of cellulose. Cellulose. Vol. 6, nº 4 (Dez. 1999), p. 265-282.pt
dc.identifier.issn0969-0239
dc.identifier.urihttp://hdl.handle.net/10400.8/76
dc.language.isoengpt
dc.publisherKluwerpt
dc.subjectCellulasept
dc.subjectCellulosept
dc.subjectDrainabilitypt
dc.subjectProtein adsorptionpt
dc.titleEffect of cellulase adsorption on the surface and interfacial properties of cellulosept
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage282pt
oaire.citation.startPage265pt
oaire.citation.titleCellulosept
person.familyNamePala
person.givenNameHelena
person.identifier.ciencia-id7B1B-7C11-411B
person.identifier.orcid0000-0001-7162-9621
person.identifier.scopus-author-id6603182617
rcaap.rightsrestrictedAccess
rcaap.typearticlept
relation.isAuthorOfPublication70de6a08-f481-4558-a268-80471bc5cc83
relation.isAuthorOfPublication.latestForDiscovery70de6a08-f481-4558-a268-80471bc5cc83

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