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A single-component hydrogel bioink for bioprinting of bioengineered 3D constructs for dermal tissue engineering

2018Journal article Restricted access
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dc.contributor.authorPereira, Rúben
dc.contributor.authorSousa, Aureliana
dc.contributor.authorBarrias, Cristina C.
dc.contributor.authorBártolo, Paulo J.
dc.contributor.authorGranja, Pedro L.
dc.date.accessioned2023-05-31T09:26:53Z
dc.date.available2023-05-31T09:26:53Z
dc.date.issued2018
dc.descriptionThis work was supported by the project Norte-01-0145-FEDER- 000012 – Structured program on bioengineered therapies for infectious diseases and tissue regeneration, supported by the Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and supported by the project P2020-PTDC/BBB ECT/2145/2014 funded by POCI via FEDER and by Foundation for Science and Technology (FCT) via OE. R. P., A. S. and C. B. thank FCT for the doctoral grant SFRH/ BD/91151/2012, the post-doctoral grant SFRH/BPD/90047/2012 and the FCT Investigator research position IF/00296/2015 (FCT and POPH/ESF), respectively. The authors thank D. S. and M. A. from CEMUP (Centro de Materiais da Universidade do Porto) for the CryoSEM and 1H NMR analyses, respectively.pt_PT
dc.description.abstractBioprinting is attractive to create cellularized constructs for skin repair. However, the vast majority of bioinks present limitations in the printing of chemically defined 3D constructs with controllable biophysical and biochemical properties. To address this challenge, a single-component hydrogel bioink with a controlled density of cell-adhesive ligands, tuneable mechanical properties and adjustable rheological behaviour is developed for extrusion bioprinting and applied for the biofabrication of 3D dermal constructs. A methacrylate modified pectin bioink is designed to allow the tethering of integrin-binding motifs and the formation of hydrogels by UV photopolymerization and ionic gelation. The rheological behaviour of a low polymer concentration (1.5 wt%) solution is adjusted by ionic crosslinking, yielding a printable bioink that holds the predesigned shape upon deposition for postprinting photocrosslinking. Printed constructs provide a suitablemicroenvironment that supports the deposition of endogenous extracellular matrix, rich in collagen and fibronectin, by entrapped dermal fibroblasts. This approach enables the design of chemically defined and cell-responsive bioinks for tissue engineering applications and particularly for the generation of biomimetic skin constructs.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPereira, R. F., Sousa, A., Barrias, C. C., Bártolo, P. J., & Granja, P. L. (2018). A single-component hydrogel bioink for bioprinting of bioengineered 3D constructs for dermal tissue engineering. Materials Horizons, 5, 1100–1111. https://doi.org/10.1039/c8mh00525gpt_PT
dc.identifier.doi10.1039/c8mh00525gpt_PT
dc.identifier.issn2051-6355
dc.identifier.urihttp://hdl.handle.net/10400.8/8540
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRoyal Society of Chemistrypt_PT
dc.relationBIOPRINTED CELULLARIZED HYDROGELS FOR IN SITU SKIN REGENERATION
dc.relationAdvanced cell therapies through bioengineered morphogenesis: High-throughput development of vascularized microtissues for regenerative medicine
dc.titleA single-component hydrogel bioink for bioprinting of bioengineered 3D constructs for dermal tissue engineeringpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBIOPRINTED CELULLARIZED HYDROGELS FOR IN SITU SKIN REGENERATION
oaire.awardTitleAdvanced cell therapies through bioengineered morphogenesis: High-throughput development of vascularized microtissues for regenerative medicine
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F90047%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00296%2F2015%2FCP1285%2FCT0002/PT
oaire.citation.endPage1111pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage1100pt_PT
oaire.citation.titleMaterials Horizonspt_PT
oaire.fundingStreamInvestigador FCT
person.familyNameBrás Pereira
person.givenNameRúben Filipe
person.identifier1337969
person.identifier.ciencia-id9417-153E-4473
person.identifier.orcid0000-0002-7255-3400
person.identifier.ridP-8503-2018
person.identifier.scopus-author-id55421048100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationeda35774-59b3-4801-aa88-9c20f4c31a78
relation.isAuthorOfPublication.latestForDiscoveryeda35774-59b3-4801-aa88-9c20f4c31a78
relation.isProjectOfPublication61565c15-16d6-46b2-95c9-d77177b9cbac
relation.isProjectOfPublication306d82a9-c0d9-4e11-8ee5-b6821c3a45b7
relation.isProjectOfPublication.latestForDiscovery306d82a9-c0d9-4e11-8ee5-b6821c3a45b7

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