A single-component hydrogel bioink for bioprinting of bioengineered 3D constructs for dermal tissue engineering

Pereira, Rúben; Sousa, Aureliana; Barrias, Cristina C.; Bártolo, Paulo J.; Granja, Pedro L.

http://hdl.handle.net/10400.8/8540

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Abstract(s)

Bioprinting is attractive to create cellularized constructs for skin repair. However, the vast majority of bioinks present limitations in the printing of chemically defined 3D constructs with controllable biophysical and biochemical properties. To address this challenge, a single-component hydrogel bioink with a controlled density of cell-adhesive ligands, tuneable mechanical properties and adjustable rheological behaviour is developed for extrusion bioprinting and applied for the biofabrication of 3D dermal constructs. A methacrylate modified pectin bioink is designed to allow the tethering of integrin-binding motifs and the formation of hydrogels by UV photopolymerization and ionic gelation. The rheological behaviour of a low polymer concentration (1.5 wt%) solution is adjusted by ionic crosslinking, yielding a printable bioink that holds the predesigned shape upon deposition for postprinting photocrosslinking. Printed constructs provide a suitablemicroenvironment that supports the deposition of endogenous extracellular matrix, rich in collagen and fibronectin, by entrapped dermal fibroblasts. This approach enables the design of chemically defined and cell-responsive bioinks for tissue engineering applications and particularly for the generation of biomimetic skin constructs.

Description

This work was supported by the project Norte-01-0145-FEDER- 000012 – Structured program on bioengineered therapies for infectious diseases and tissue regeneration, supported by the Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and supported by the project P2020-PTDC/BBB ECT/2145/2014 funded by POCI via FEDER and by Foundation for Science and Technology (FCT) via OE. R. P., A. S. and C. B. thank FCT for the doctoral grant SFRH/ BD/91151/2012, the post-doctoral grant SFRH/BPD/90047/2012 and the FCT Investigator research position IF/00296/2015 (FCT and POPH/ESF), respectively. The authors thank D. S. and M. A. from CEMUP (Centro de Materiais da Universidade do Porto) for the CryoSEM and 1H NMR analyses, respectively.

URI

http://hdl.handle.net/10400.8/8540

Citation

Pereira, R. F., Sousa, A., Barrias, C. C., Bártolo, P. J., & Granja, P. L. (2018). A single-component hydrogel bioink for bioprinting of bioengineered 3D constructs for dermal tissue engineering. Materials Horizons, 5, 1100–1111. https://doi.org/10.1039/c8mh00525g