Repository logo
 
Publication

Assessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responses

2019-11Journal article Restricted access
Simple item page
dc.contributor.authorMonteiro, Hugo R.
dc.contributor.authorPestana, João L. T.
dc.contributor.authorNovais, Sara C.
dc.contributor.authorLeston, Sara
dc.contributor.authorRamos, Fernando
dc.contributor.authorSoares, Amadeu M. V. M.
dc.contributor.authorDevreese, Bart
dc.contributor.authorLemos, Marco F. L.
dc.date.accessioned2019-10-16T10:53:53Z
dc.date.available2019-10-16T10:53:53Z
dc.date.issued2019-11
dc.date.updated2019-10-07T10:35:53Z
dc.descriptionThis study had the support of the Fundação para a Ciência e a Tecnologia (FCT) through MARE: UID/MAR/04292/2019; CESAM: UID/ AMB/50017/2019, FCT/MCTES through national funds, the cofunding by the FEDER within the PT2020 Partnership Agreement and Compete 2020; Project PROTEOME (PTDC/AAG-MAA/1302/2014) cofinanced by COMPETE (POCI-01-0145-FEDER-016773). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) co-funded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund. Hugo R. Monteiro, Sara C. Novais, and João L.T. Pestana wish to acknowledge the financial support given by FCT (SFRH/BD/80988/2011, SFRH/BPD/94500/2013, IF/01420/2015, respectively).
dc.description.abstractFipronil is a phenylpyrazole insecticide that entered the market to replace organochlorides and organophosphates. Fipronil impairs the regular inhibition of nerve impulses that ultimately result in paralysis and death of insects. Because of its use as a pest control, and due to runoff events, fipronil has been detected in freshwater systems near agricultural areas, and therefore might represent a threat to non-target aquatic organisms. In this study, the toxicity of fipronil to the freshwater midge Chironomus riparius was investigated at biochemical, molecular, and whole organism (e.g. growth, emergence, and behavior) levels. At the individual level, chronic (28 days) exposure to fipronil resulted in reduced larval growth and emergence with a lowest observed effect concentration (LOEC) of 0.081 μg L−1. Adult weight, which is directly linked to the flying performance and fecundity of midges, was also affected (LOEC = 0.040 μg L−1). Additionally, behavioral changes such as irregular burrowing behavior of C. riparius larvae (EC50 = 0.084 μg L−1) and impairment of adult flying performance were observed. At a biochemical level, acute (48 h) exposure to fipronil increased cellular oxygen consumption (as indicated by the increase of electron transport system (ETS) activity) and decreased antioxidant and detoxification defenses (as suggested by the decrease in catalase (CAT) and glutathione S-transferase (GST) activities). Exposure to fipronil also caused alterations in the fatty acid profile of C. riparius, since high levels of stearidonic acid (SDA) were observed. A comparison between exposed and nonexposed larvae also revealed alterations in the expression of globins, cytoskeleton and motor proteins, and proteins involved in protein biosynthesis. These alterations may aid in the interpretation of potential mechanisms of action that lead to the effects observed at the organism level. Present results show that environmentally relevant concentrations of fipronil are toxic to chironomid populations which call for monitoring of phenylpyrazole insecticides and of their ecological effects in freshwaters. Present results also emphasize the importance of complementing ecotoxicological data with molecular approaches such as proteomics, for a better interpretation of the mode of action of insecticides in aquatic invertebrates.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMonteiro, H. R., Pestana, J. L. T., Soares, A. M. V. M., Novais, S. C., Lemos, M. F. L., Devreese, B., … Ramos, F. (2019). Assessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responses. Aquatic Toxicology, 216. https://doi.org/10.1016/j.aquatox.2019.105292pt_PT
dc.identifier.doi10.1016/j.aquatox.2019.105292
dc.identifier.issn0166-445X
dc.identifier.slugcv-prod-760979
dc.identifier.urihttp://hdl.handle.net/10400.8/4213
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMARE - Marine and Environmental Sciences Centre
dc.relationCentre for Environmental and Marine Studies
dc.relationProTEoME - PROteomic Tools to assess Endocrine disruptiOn MEchanisms
dc.relationPROTEOME - PROTEOMIC TOOLS TO ASSESS ENDOCRINE DISRUPTION MECHANISTIC ECOTOXICITY
dc.relationAn integrative approach to assess the knock-on effects of psychiatric drugs in the freshwater environment
dc.relation.ispartofAquatic Toxicologyen_US
dc.subjectAquatic insectspt_PT
dc.subjectBiomarkerspt_PT
dc.subjectEnvironmental proteomicspt_PT
dc.subjectPhenylpyrazole insecticidespt_PT
dc.subjectSub-lethal toxicitypt_PT
dc.titleAssessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responsespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMARE - Marine and Environmental Sciences Centre
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleProTEoME - PROteomic Tools to assess Endocrine disruptiOn MEchanisms
oaire.awardTitlePROTEOME - PROTEOMIC TOOLS TO ASSESS ENDOCRINE DISRUPTION MECHANISTIC ECOTOXICITY
oaire.awardTitleAn integrative approach to assess the knock-on effects of psychiatric drugs in the freshwater environment
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMAR%2F04292%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAMB%2F50017%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FAAG-MAA%2F1302%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F80988%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F94500%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F01420%2F2015%2FCP1302%2FCT0016/PT
oaire.citation.startPage105292pt_PT
oaire.citation.titleAquatic Toxicologypt_PT
oaire.citation.volume216pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamSFRH
oaire.fundingStreamInvestigador FCT
person.familyNameMonteiro
person.familyNameNovais
person.familyNameLemos
person.givenNameHugo Ricardo
person.givenNameSara
person.givenNameMarco
person.identifier996337
person.identifier.ciencia-id3415-56EB-358E
person.identifier.ciencia-id2216-A2E0-A118
person.identifier.orcid0000-0003-4913-1194
person.identifier.orcid0000-0003-1306-3396
person.identifier.orcid0000-0001-9887-1864
person.identifier.ridC-7853-2012
person.identifier.ridA-9032-2012
person.identifier.ridF-7951-2011
person.identifier.scopus-author-id56487254800
person.identifier.scopus-author-id23025463300
person.identifier.scopus-author-id7006042884
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid971F-ACCA-C0D1 | Marco Lemos
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationd0845585-ad16-4565-adb0-63148ffb543c
relation.isAuthorOfPublicationde69a91d-24ba-4155-ad23-76367cca2232
relation.isAuthorOfPublicationf21e5540-df76-43e9-ad64-93edd70da1f1
relation.isAuthorOfPublication.latestForDiscoveryf21e5540-df76-43e9-ad64-93edd70da1f1
relation.isProjectOfPublicationa6f839e6-c0f3-4bd6-aca0-3db7b9e2c74c
relation.isProjectOfPublication371043d5-578c-4024-9ee4-18a711e78df6
relation.isProjectOfPublication4e0a9ed1-4c4e-4869-a92e-b9c3b9527c79
relation.isProjectOfPublication46c02362-bd97-45d0-86c9-6bb4cb18a9eb
relation.isProjectOfPublication181f3029-c366-48c4-afa6-0fe703e76db7
relation.isProjectOfPublication55e0ad86-600b-4ae8-bf7d-2d7143e3e172
relation.isProjectOfPublication.latestForDiscovery371043d5-578c-4024-9ee4-18a711e78df6

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Monteiro_et_al_(2019)_Aquatic_Tox.pdf
Size:
1.42 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.33 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

MARE - Artigos com peer review
ESTM - Artigos em revistas internacionais