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Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes

2020-08-22Journal article Restricted access
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dc.contributor.authorCampos, António
dc.contributor.authorCampos, Elisa J.
dc.contributor.authorMartins, João
dc.contributor.authorRodrigues, Flávia S.C.
dc.contributor.authorSilva, Rufino
dc.contributor.authorAmbrósio, António Francisco
dc.date.accessioned2022-02-11T14:51:49Z
dc.date.available2022-02-11T14:51:49Z
dc.date.issued2020-08-22
dc.description.abstractIncreasing evidence points to inflammation as a key factor in the pathogenesis of diabetic retinopathy (DR). Choroidal inflammatory changes in diabetes have been reported and in vivo choroidal thickness (CT) has been searched as a marker of retinopathy with contradictory results. We aimed to investigate the early stages in the retina and choroid in an animal model of Type 1 diabetes. Type 1 diabetes was induced in male Wistar rats via a single i.p. streptozotocin injection. At 8 weeks after disease onset, CT, choroidal vascular density, VEGF and VEGFR2 expression, microglial cell and pericyte distribution were evaluated. Diabetic rats showed no significant change in CT and choroidal vascular density. A widened pericyte-free gap between the retinal pigment epithelium and the choroid was observed in diabetic rats. The immunoreactivity of VEGFR2 was decreased in the retina of diabetic rats, despite no statistically significant difference in the immunoreactivity of VEGF. The density of microglial cells significantly increased in the choroid and retina of diabetic rats. Reactive microglial cells were found to be more abundant in the choroid of diabetic rats. Evidences of the interconnection between the superficial, intermediate, and deep plexuses of the retina were also observed. At early stages, Type 1 diabetes does not affect choroidal thickness and choroidal vascular density. Proliferation and reactivity of microglial cells occurs in the choroidal stroma and the retina. The expression of VEGFR2 decreases in the retina.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCampos, A., Campos, E. J., Martins, J., Rodrigues, F. S. C., Silva, R., & Ambrósio, A. F. (2020). Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes. Experimental Eye Research, 199, 108195. https://doi.org/10.1016/j.exer.2020.108195pt_PT
dc.identifier.doi10.1016/j.exer.2020.108195pt_PT
dc.identifier.issn0014-4835
dc.identifier.urihttp://hdl.handle.net/10400.8/6658
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCNC. IBILI
dc.relationCNC. IBILI
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S001448352030453X?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectChoroidpt_PT
dc.subjectRetinapt_PT
dc.subjectType 1 diabetespt_PT
dc.subjectChoroidal thicknesspt_PT
dc.subjectMicrogliapt_PT
dc.titleInflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCNC. IBILI
oaire.awardTitleCNC. IBILI
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FNEU%2F04539%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FNEU%2F04539%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FNEU%2F04539%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04539%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04539%2F2020/PT
oaire.citation.titleExperimental Eye Researchpt_PT
oaire.citation.volume199: 108195pt_PT
oaire.fundingStream5876
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCampos de Figueiredo
person.givenNameAntónio
person.identifier.ciencia-id4F1C-D822-7CFF
person.identifier.orcid0000-0003-0490-5889
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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