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Publication
Multifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration
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Advisor(s)
Abstract(s)
The similarities of electrospun fibers with the skin extracellular matrix (ECM) make them
promising structures for advanced wound dressings. Moreover, infection and resistance in wounds
are a major health concern that may be reduced with antibacterial wound dressings. In this work, a
multifunctional wound dressing was developed based on gelatin/chitosan hybrid fibers dopped with
phlorotannin-enrich extract from the seaweed Undaria pinnatifida. The intrinsic electrospun structure
properties combined with the antimicrobial and anti-inflammatory properties of phlorotannin-enrich
extract will enhance the wound healing process. Electrospun meshes were produced by incorporating
1 or 2 wt% of extract, and the structure without extract was used as a control. Physico-chemical,
mechanical, and biological properties were evaluated for all conditions. Results demonstrated that
all developed samples presented a homogenous fiber deposition with the average diameters closer
to the native ECM fibrils, and high porosities (~90%) that will be crucial to control the wound
moist environment. According to the tensile test assays, the incorporation of phlorotannin-enriched
extract enhances the elastic performance of the samples. Additionally, the extract incorporation
made the structure stable over time since its in vitro degradation rates decreased under enzymatic
medium. Extract release profile demonstrated a longstanding delivery (up to 160 days), reaching
a maximum value of ~98% over time. Moreover, the preliminary antimicrobial results confirm the
mesh’s antimicrobial activity against Pseudomonas aeruginosa and Staphylococcus aureus. In terms of
biological characterization, no condition presented cytotoxicity effects on hDNF cells, allowing their
adhesion and proliferation over 14 days, except the condition of 2 wt% after 7 days. Overall, the
electrospun structure comprising phlorotannins-enriched extract is a promising bioactive structure
with potential to be used as a drug delivery system for skin regeneration by reducing the bacterial
infection in the wound bed.
Description
Keywords
Chronic wounds Chitosan/gelatin/ phlorotannin-enriched extract Electrospun wound dressing Multifunctional structures Wound healing
Citation
Ferreira, C.A.M.; Januário, A.P.; Félix, R.; Alves, N.; Lemos, M.F.L.; Dias, J.R. Multifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration. Pharmaceutics 2021, 13, 2152. https://doi.org/10.3390/ pharmaceutics13122152
Publisher
MDPI